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SARS-CoV-2 (COVID-19) Spike S1抗体 [HL1]

Recombinant antibody
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1] used in ELISA (ELISA). GTX635656
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1] used in Western Blot (WB). GTX635656
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1] used in ELISA (ELISA). GTX635656
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1] used in ELISA (ELISA). GTX635656
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1] used in Western Blot (WB). GTX635656
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1] used in ELISA (ELISA). GTX635656
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1] used in ELISA (ELISA). GTX635656
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635656

目录号 GTX635656

文献引用 5

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL1

同种型

 IgG

实验应用

 WB, ICC/IF, IP, ELISA, Sandwich ELISA

种属反应

 SARS Coronavirus 2
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 SARS-CoV-2 (COVID-19) Spike 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IP Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX632604, Detection: GTX635656
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

产品说明

This antibody detects SARS-CoV-2 Spike protein, but does not cross-react with SARS-CoV or MERS-CoV spike proteins based on our internal testing.

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

SARS Coronavirus 2

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminal region of SARS-CoV-2 (COVID-19) spike (SARS-CoV-2 (strain Wuhan-Hu-1)). The exact sequence is proprietary.

纯化方式

Affinity purified by protein A.

偶联

Unconjugated

RRID

AB_2888549

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

2019-nCoV Spike , SARS-CoV-2 Spike , COVID-19 Spike

研究领域

產品图片

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1] used in ELISA (ELISA). GTX635656

GTX635656 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein or recombinant SARS-CoV spike S1 subunit protein (62.5-4000 ng/mL). Coated protein probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1] (GTX635656) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1] used in Western Blot (WB). GTX635656

GTX635656 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1] (GTX635656) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1] used in ELISA (ELISA). GTX635656

GTX635656 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) protein (GTX135817-pro) (4000-62.5 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1] (GTX635656) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1] used in ELISA (ELISA). GTX635656

GTX635656 ELISA Image

Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein at concentrations ranging from 0.0625 μg/mL to 4 μg/mL. The coated protein is detected with (GTX635656) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1] used in Western Blot (WB). GTX635656

GTX635656 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1] (GTX635656) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1] used in ELISA (ELISA). GTX635656

GTX635656 ELISA Image

Sandwich ELISA detection of non-transfected and SARS-CoV-2 spike (full length) transfected 293T whole cell extracts (GTX535664) using GTX632604 as capture antibody at concentration of 5 μg/mL and GTX635656 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1] used in ELISA (ELISA). GTX635656

GTX635656 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1] (GTX635656) (2.4*10⁻⁶ ~ 20 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.
EC50 : 0.117 nM

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635656

GTX635656 ICC/IF Image

SARS-CoV-2 (COVID-19) spike antibody [HL1] detects SARS-CoV-2 (COVID-19) spike protein by immunofluorescent analysis.
Sample: Mock and SARS-CoV-2 Spike-transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) spike stained by SARS-CoV-2 (COVID-19) spike antibody [HL1] (GTX635656) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

文献引用

Application Reference

Y Maeda et al. Int J Mol Sci 2024; 25 (2) : 1353 Differential Ability of Spike Protein of SARS-CoV-2 Variants to Downregulate ACE2
实验应用 : WB, IP
Y Maeda et al. Epidemiol Infect 2023; Differential Ability of S Protein of SARSCoV-2 Variants to Downregulate ACE2
实验应用 : WB, IP
Petros BA et al. Med (N Y) 2022; Multimodal surveillance of SARS-CoV-2 at a university enables development of a robust outbreak response framework.
Yen-Chung Lai et al. Front Immunol 2022; 13 : 868724 Antigenic Cross-Reactivity Between SARS-CoV-2 S1-RBD and Its Receptor ACE2.
实验应用 : WB
Leonid Yurkovetskiy et al. Cell 2020; S0092-8674 (20) : 31229-0 Structural and Functional Analysis of the D614G SARS-CoV-2 Spike Protein Variant.
实验应用 : WB
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¥ 1700
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