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SARS-CoV-2 (COVID-19) Spike S1抗体 [HL134]

Recombinant antibody
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL134] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635671
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL134] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635671
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL134] used in ELISA (ELISA). GTX635671
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL134] used in Western Blot (WB). GTX635671
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL134] used in ELISA (ELISA). GTX635671
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL134] used in Western Blot (WB). GTX635671
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL134] used in ELISA (ELISA). GTX635671

目录号 GTX635671

文献引用 2

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL134

同种型

 IgG

实验应用

 WB, ICC/IF, ELISA, IHC-P (cell pellet)

种属反应

 SARS Coronavirus 2
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 SARS-CoV-2 (COVID-19) Spike 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
ELISA Assay dependent
IHC-P (cell pellet) Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

产品说明

This antibody detects SARS-CoV-2 Spike protein, but does not cross-react with SARS-CoV or MERS-CoV spike proteins based on our internal testing.

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

SARS Coronavirus 2

免疫原

Recombinant protein encompassing a sequence within the N-terminus region of SARS-CoV-2 (COVID-19) spike (S1) (SARS-CoV-2 (strain Wuhan-Hu-1)). The exact sequence is proprietary.

纯化方式

Affinity purified by protein A.

偶联

Unconjugated

RRID

AB_2888550

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

2019-nCoV Spike , SARS-CoV-2 Spike , COVID-19 Spike

研究领域

產品图片

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL134] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635671

GTX635671 ICC/IF Image

SARS-CoV-2 (COVID-19) Spike S1 antibody [HL134] detects SARS-CoV-2 (COVID-19) Spike protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) Spike stained by SARS-CoV-2 (COVID-19) Spike S1 antibody [HL134] (GTX635671) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL134] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635671

GTX635671 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) Spike S1 antibody [HL134] detects SARS-CoV-2 (COVID-19) Spike S1 protein by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Spike transfected 293T cell FFPE Cell Pellet Block (GTX435640).
Green: SARS-CoV-2 (COVID-19) Spike S1 stained by SARS-CoV-2 (COVID-19) Spike S1 antibody [HL134] (GTX635671) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL134] used in ELISA (ELISA). GTX635671

GTX635671 ELISA Image

Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein, and SARS-CoV spike S1 subunit protein at concentrations ranging from 0.0625 μg/mL to 4 μg/mL. The coated protein is detected with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL134] (GTX635671) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL134] used in Western Blot (WB). GTX635671

GTX635671 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL134] (GTX635671) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL134] used in ELISA (ELISA). GTX635671

GTX635671 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL134] (GTX635671) (2.4*10⁻⁶ ~ 20 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.
EC50 : 0.552 nM

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL134] used in Western Blot (WB). GTX635671

GTX635671 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL134] (GTX635671) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL134] used in ELISA (ELISA). GTX635671

GTX635671 ELISA Image

Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein, and SARS-CoV spike S1 subunit protein at concentrations ranging from 0.0625 μg/mL to 4 μg/mL. The coated protein is detected with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL134] (GTX635671) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

文献引用

Application Reference

Hussain H et al. Microorganisms 2024; 12 (2) Dermatologic Changes in Experimental Model of Long COVID.
Paidas MJ et al. Front Pharmacol 2022; 13 : 864798 Mechanism of Multi-Organ Injury in Experimental COVID-19 and Its Inhibition by a Small Molecule Peptide.
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产品包装 报价 (¥)
¥ 4000
¥ 1700
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