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SARS-CoV-2 (COVID-19) Spike S1抗体 [HL6]

Recombinant antibody
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in ELISA (ELISA). GTX635654
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in ELISA (ELISA). GTX635654
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in IHC (Paraffin sections) (IHC-P). GTX635654
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in ELISA (ELISA). GTX635654
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in Western Blot (WB). GTX635654
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in ELISA (ELISA). GTX635654
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in ELISA (ELISA). GTX635654
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in ELISA (ELISA). GTX635654
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635654
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635654
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in Flow cytometry (FACS). GTX635654
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635654
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in IHC (Paraffin sections) (IHC-P). GTX635654
Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in Western Blot (WB). GTX635654

目录号 GTX635654

文献引用 20

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL6

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, FACS, ELISA, Sandwich ELISA, IHC-P (cell pellet)

种属反应

 SARS Coronavirus 2
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 SARS-CoV-2 (COVID-19) Spike 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:500
FACS Assay dependent
ELISA Assay dependent
Sandwich ELISA Assay dependent
IHC-P (cell pellet) Assay dependent

Note :

IHC-P
Recommended heat-Induced Epitope Retrieval pH 6.0 for 20 minutes , background Eraser (BGE) blocking step, and DAB Enhancer.

Sandwich ELISA
Capture : GTX635807, Detection : GTX636042 or Capture : GTX636042, Detection : GTX635807 / GTX635693 / GTX635713 / GTX635654.
Please notice that the capture antibody needs to be conjugated to HRP to function as the detection antibody when paired with the detected antibody. Please contact us for custom HRP-conjugated antibody.
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

产品说明

This antibody detects SARS-CoV-2 Spike protein, but does not cross-react with SARS-CoV or MERS-CoV spike proteins based on our internal testing.

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

SARS Coronavirus 2

免疫原

Recombinant protein encompassing a sequence within the N-terminus region of SARS-CoV-2 (COVID-19) spike (S1) (SARS-CoV-2 (strain Wuhan-Hu-1)). The exact sequence is proprietary.

纯化方式

Affinity purified by protein A.

偶联

Unconjugated

RRID

AB_2888548

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

2019-nCoV Spike , SARS-CoV-2 Spike , COVID-19 Spike

研究领域

產品图片

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in ELISA (ELISA). GTX635654

GTX635654 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein or recombinant SARS-CoV spike S1 subunit protein (62.5-4000 ng/mL). Coated protein probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in ELISA (ELISA). GTX635654

GTX635654 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX135817-pro) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) Spike RBD antibody [GT5449] (GTX636042) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) (1 μg/mL)

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in IHC (Paraffin sections) (IHC-P). GTX635654

GTX635654 IHC-P Image

SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) detects SARS-CoV-2 (COVID-19) Spike S1 protein by immunohistochemical analysis of SARS-CoV-2 infected human placenta (left) and normal human placenta (right).

The IHC-P was performed by HISTOWIZ.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in ELISA (ELISA). GTX635654

GTX635654 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 spike (trimer) protein using antibodies as below.

Capture: SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) (5 μg/mL)
Detection: SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) (1 μg/mL)

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in Western Blot (WB). GTX635654

GTX635654 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in ELISA (ELISA). GTX635654

GTX635654 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) (2.4*10⁻⁶ ~ 20 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.
EC50 : 0.251 nM

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in ELISA (ELISA). GTX635654

GTX635654 ELISA Image

Indirect ELISA analysis was performed by coating plate with 50 μL of recombinant SARS-CoV-2 (COVID-19) spike S1 subunit protein at concentrations ranging from 0.0625 μg/mL to 4 μg/mL. The coated protein is detected with (GTX635654) at 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in ELISA (ELISA). GTX635654

GTX635654 ELISA Image

Sandwich ELISA detection of non-transfected and SARS-CoV-2 spike (full length) transfected 293T whole cell extracts (GTX535664) using GTX632604 as capture antibody at concentration of 5 μg/mL and GTX635654 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635654

GTX635654 ICC/IF Image

SARS-CoV-2 (COVID-19) spike antibody [HL6] detects SARS-CoV-2 (COVID-19) spike protein by immunofluorescent analysis.
Sample: Mock and SARS-CoV-2 Spike-transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) spike stained by SARS-CoV-2 (COVID-19) spike antibody [HL6] (GTX635654) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635654

GTX635654 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] detects SARS-CoV-2 (COVID-19) Spike S1 protein by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Spike transfected 293T cell FFPE Cell Pellet Block (GTX435640).
Green: SARS-CoV-2 (COVID-19) Spike S1 stained by SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in Flow cytometry (FACS). GTX635654

GTX635654 FACS Image

SARS-CoV-2 (COVID-19) spike antibody [HL6] (GTX635654) detects SARS-CoV-2 (COVID-19) spike protein by flow cytometry analysis.
Sample: 293T cells transfected SARS-CoV-2 (2019-nCoV) Spike.
Blue: Unlabelled sample was used as a control.
Red: SARS-CoV-2 (COVID-19) spike antibody [HL6] (GTX635654) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635654

GTX635654 ICC/IF Image

SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] detects SARS-CoV-2 (COVID-19) Spike S1 protein at cytoplasm by immunofluorescent analysis.
Sample: Mock and transfected transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) Spike S1 stained by SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) diluted at 1:2000.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in IHC (Paraffin sections) (IHC-P). GTX635654

GTX635654 IHC-P Image

SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) detects SARS-CoV-2 (COVID-19) Spike S1 protein by immunohistochemical analysis of SARS-CoV-2 infected human lung.

The IHC-P was performed by HISTOWIZ.

Anti-SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] used in Western Blot (WB). GTX635654

GTX635654 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike S1 antibody [HL6] (GTX635654) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

文献引用

Application Reference

T Iida et al. Research Square 2024; High-throughput Light-induced Immunoassay under One-minute Antibody-coating with Energy Saving Nanoparticle-imprinted Substrate
K Miura et al. J Virol 2023; Distinct motifs in the E protein are required for SARS-CoV-2 virus particle formation and lysosomal deacidification in host cells
实验应用 : WB
C Di Primio et al. PNAS Nexus 2023; 2 (9) : pgad282 Severe acute respiratory syndrome coronavirus 2 infection leads to Tau pathological signature in neurons
实验应用 : WB
Liu JF et al. EBioMedicine 2023; 90 : 104518 Proteomic and phosphoproteomic characteristics of the cortex, hippocampus, thalamus, lung, and kidney in COVID-19-infected female K18-hACE2 mice.
Piranej S et al. bioRxiv 2023; Rolosense: Mechanical detection of SARS-CoV-2 using a DNA-based motor.
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Application Reference
T Iida et al. Research Square 2024; High-throughput Light-induced Immunoassay under One-minute Antibody-coating with Energy Saving Nanoparticle-imprinted Substrate
K Miura et al. J Virol 2023; Distinct motifs in the E protein are required for SARS-CoV-2 virus particle formation and lysosomal deacidification in host cells
实验应用 : WB
C Di Primio et al. PNAS Nexus 2023; 2 (9):pgad282 Severe acute respiratory syndrome coronavirus 2 infection leads to Tau pathological signature in neurons
实验应用 : WB
Liu JF et al. EBioMedicine 2023; 90:104518 Proteomic and phosphoproteomic characteristics of the cortex, hippocampus, thalamus, lung, and kidney in COVID-19-infected female K18-hACE2 mice.
Piranej S et al. bioRxiv 2023; Rolosense: Mechanical detection of SARS-CoV-2 using a DNA-based motor.
Reyes S et al. Sens Diagn 2022; 1 (6):1198-1208 Metal organic framework encapsulated tamavidin-Gluc reporter: application in COVID-19 spike antigen bioluminescent immunoassay.
实验应用 : Sandwich ELISA
Padula L et al. Vaccine X 2022; 12:100202 Secreted heat shock protein gp96-Ig and OX40L-Fc combination vaccine enhances SARS-CoV-2 Spike (S) protein-specific B and T cell immune responses.
实验应用 : ICC/IF
Chuang HC et al. EMBO Mol Med 2022; 14 (9):e15904 SARS-CoV-2 spike protein enhances MAP4K3/GLK-induced ACE2 stability in COVID-19.
Nasser H et al. STAR Protoc 2022; 3 (4):101773 Monitoring fusion kinetics of viral and target cell membranes in living cells using a SARS-CoV-2 spike-protein-mediated membrane fusion assay.
Yuchao Chen et al. Sci Adv 2022; 8 (21):eabn3481 Quantitative and ultrasensitive in situ immunoassay technology for SARS-CoV-2 detection in saliva.
Wei Deng et al. Signal Transduct Target Ther 2022; 7 (1):124 Sequential immunizations confer cross-protection against variants of SARS-CoV-2, including Omicron in Rhesus macaques.
实验应用 : IHC-P
Sarah Reagan-Steiner et al. Emerg Infect Dis 2022; 28 (3):510-517 Detection of SARS-CoV-2 in Neonatal Autopsy Tissues and Placenta.
实验应用 : IHC-P
Kim HK et al. Biomaterials 2022; 283:121460 Generation of human tonsil epithelial organoids as an ex vivo model for SARS-CoV-2 infection.
实验应用 : WB
Song Z et al. Signal Transduct Target Ther 2022; 7 (1):29 Integrated histopathological, lipidomic, and metabolomic profiles reveal mink is a useful animal model to mimic the pathogenicity of severe COVID-19 patients.
实验应用 : IHC-P
Park SB et al. mBio 2022;:e0323821 Targeting the Fusion Process of SARS-CoV-2 Infection by Small Molecule Inhibitors.
Luke Lee et al. Res Sq 2021; Quantitative and Ultrasensitive In-situ Immunoassay Technology for SARS-CoV-2 Detection in Saliva.
Saito A et al. Nature 2021; Enhanced fusogenicity and pathogenicity of SARS-CoV-2 Delta P681R mutation.
实验应用 : ICC/IF, FACS
Danics K et al. Geroscience 2021; (Epub) A COVID-19-association-dependent categorization of death causes in 100 autopsy cases.
实验应用 : IHC-P
Man?ek-Keber M et al. FASEB J 2021; 35 (6):e21651 Disruption of disulfides within RBD of SARS-CoV-2 spike protein prevents fusion and represents a target for viral entry inhibition by registered drugs.
实验应用 : FACS
Motozono C. et al. Cell Host Microbe . 2021; 29 (7):1124-1136 SARS-CoV-2 spike L452R variant evades cellular immunity and increases infectivity.
实验应用 : FACS
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产品包装 报价 (¥)
¥ 4000
¥ 1700
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