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SARS-CoV-2 (COVID-19) Spike S1 蛋白, His tag (active)

SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active). GTX01554-pro
SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active). GTX01554-pro
SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active). GTX01554-pro
SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active). GTX01554-pro

目录号 GTX01554-pro

实验应用

 ELISA, Functional Assay

Speciess

 SARS Coronavirus 2
产品包装
100 μg (¥6700)
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产品应用

应用说明

Activity was measured by its binding ability in a functional ELISA. Immobilized Recombinant Human ACE2 at 2 μg/ml (100 μl/well ) can bind Recombinant nCoV-S1. The EC₅₀ of SARS-CoV-2 (COVID-19) Spike S1 protein is 0.25-0.45 μg/ml.

产品属性

形式

Lyophilized powder

存储溶液

Reconstitute with distilled water to 0.1-0.5mg/ml. Lyophilized from 0.22μm-filtered PBS (pH7.4).

保存剂

No preservative

存放说明

Store at -20ºC or below. After reconstitution, the protein solution is stable at -20 ºC for 1 month, at 4ºC for up to 1 week. Avoid freeze-thaw cycles.

Region/Sequence

Val16-Arg685 of SARS-CoV-2 (COVID-19) Spike S1 protein (Accession #YP_009724390.1) fused with a 6×His tag at the C-terminus.

表达系统

HEK293 cells

纯度

> 90% by SDS-PAGE

内毒素

< 1.0 EU/μg (determined by the LAL method)

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

SARS-CoV-2 Spike , COVID-19 Spike

数据库

研究领域

產品图片

SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active). GTX01554-pro

GTX01554-pro ELISA Image

Inhibition analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX01554-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein, mouse IgG Fc tag (GTX135683-pro) (1000 ng/mL). ACE2 binding was inhibited by increasing concentrations of SARS-CoV-2 (COVID-19) Spike RBD antibody (GTX135709) (14-10000 ng/mL). Bound ACE2 protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active). GTX01554-pro

GTX01554-pro ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) protein (GTX01554-pro) (4000-62.5 ng/mL). Coated protein probed with SARS-CoV-2 (COVID-19) Spike S1 antibody (GTX135384) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.

SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active). GTX01554-pro

GTX01554-pro Functional Assay Image

Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active) (GTX01554-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag (GTX135683-pro) (14-10000 ng/mL). Bound protein was detected by Goat Anti-Mouse IgG antibody (HRP) (GTX213111-01) (1:10000).

SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active). GTX01554-pro

GTX01554-pro Image

SDS-PAGE of GTX01554-pro SARS-CoV-2 (COVID-19) Spike S1 protein, His tag (active).

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SDS
PBS.pdf
产品包装 报价 (¥)
¥ 6700
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