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SARS-CoV-2 (COVID-19) Spike overexpression 293T whole 细胞裂解物

SARS-CoV-2 (COVID-19) Spike overexpression 293T whole cell lysate. GTX535664
SARS-CoV-2 (COVID-19) Spike overexpression 293T whole cell lysate. GTX535664
SARS-CoV-2 (COVID-19) Spike overexpression 293T whole cell lysate. GTX535664

目录号 GTX535664

实验应用

WB, ELISA

Speciess

SARS Coronavirus 2
产品包装
200 μg (¥3500)
产品说明

摘要

Whole-cell lysate of 293T cells overexpressing SARS-CoV-2 spike protein (full-length, no tag). The translated amino acid sequence is based on QHD43416.1. Lysate prepared in RIPA buffer with no reducing agents and is unboiled. Please see matched control 293T lysate (GTX535673)

产品属性

形式

Liquid

存储溶液

50 mM Tris-HCl (pH8.0), 150 mM NaCl, 0.5% sodium deoxycholate, 1% Triton X-100, 0.25% SDS, 1 mM PMSF, 5 μg/ml Leupeptin, 2 μg/ml Aprotinin, 1 μg/ml Pepstatin A

保存剂

0.025% ProClin 300

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

2019-nCoV Spike , SARS-CoV-2 Spike , COVID-19 Spike

研究领域

產品图片

SARS-CoV-2 (COVID-19) Spike overexpression 293T whole cell lysate. GTX535664

GTX535664 WB Image

Non-transfected and transfected 293T whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) Spike antibody (GTX135356) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SARS-CoV-2 (COVID-19) Spike overexpression 293T whole cell lysate. GTX535664

GTX535664 WB Image

SARS-CoV-2 (COVID-19) spike 293T overexpression cell lysates (GTX535664) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) spike antibody [1A9] (GTX632604) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

SARS-CoV-2 (COVID-19) Spike overexpression 293T whole cell lysate. GTX535664

GTX535664 ELISA Image

Sandwich ELISA detection of non-transfected (GTX535673) and SARS-CoV-2 spike (full length) transfected (GTX535664) 293T whole cell extracts using SARS-CoV-2 (COVID-19) Spike S1 antibody [GT263] (GTX635708) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) Spike S1 antibody [HL263] (GTX635672) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

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SDS
Proclin.pdf
产品包装 报价 (¥)
¥ 3500
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