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SARS-CoV-2 (COVID-19) Nucleocapsid抗体 [HL146]

Recombinant antibody
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL146] used in ELISA (ELISA). GTX635680
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL146] used in ELISA (ELISA). GTX635680
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL146] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635680
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL146] used in Western Blot (WB). GTX635680
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL146] used in ELISA (ELISA). GTX635680
Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL146] used in Western Blot (WB). GTX635680

目录号 GTX635680

文献引用 1

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL146

同种型

 IgG

实验应用

 WB, ICC/IF, ELISA, Sandwich ELISA

种属反应

 SARS Coronavirus, SARS Coronavirus 2
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 SARS-CoV-2 (COVID-19) nucleocapsid 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
ELISA Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX635680, Detection : GTX635678 / GTX635688.
Please notice that GTX635688 / GTX635678 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635680. Please contact us for custom HRP-conjugated antibody.
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

产品说明

This antibody detects both SARS-CoV nucleocapsid and SARS-CoV-2 nucleocapsid proteins. Our internal testing indicates no cross-reactivity with MERS-CoV nucleocapsid protein.

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

SARS Coronavirus 2

免疫原

Full length SARS-CoV-2 (COVID-19) nucleocapsid Recombinant protein. (SARS-CoV-2 (strain Wuhan-Hu-1))

纯化方式

Affinity purified by protein A.

偶联

Unconjugated

RRID

AB_2888554

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

2019-nCoV Nucleocapsid , SARS-CoV-2 Nucleocapsid , COVID-19 Nucleocapsid , 2019-nCoV NP , SARS-CoV-2 NP , COVID-19 NP

研究领域

產品图片

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL146] used in ELISA (ELISA). GTX635680

GTX635680 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL146] (GTX635680) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL249] (GTX635678) as detection antibody at concentration of 1 μg/mL.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL146] used in ELISA (ELISA). GTX635680

GTX635680 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL146] (GTX635680) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) as detection antibody at concentration of 1 μg/mL.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL146] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635680

GTX635680 ICC/IF Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL146] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL146] (GTX635680) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL146] used in Western Blot (WB). GTX635680

GTX635680 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL146] (GTX635680) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL146] used in ELISA (ELISA). GTX635680

GTX635680 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro) (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL146] (GTX635680) (2.4*10⁻⁶ ~ 20 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.
EC50 : 0.05 nM

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL146] used in Western Blot (WB). GTX635680

GTX635680 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL146] (GTX635680) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

文献引用

Application Reference

Niemeyer D et al. PLoS Biol 2022; 20 (11) : e3001871 SARS-CoV-2 variant Alpha has a spike-dependent replication advantage over the ancestral B.1 strain in human cells with low ACE2 expression.
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产品包装 报价 (¥)
¥ 4000
¥ 1700
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