SARS-CoV-2 (COVID-19) Nucleocapsid抗体 [HL448]

Name: SARS-CoV-2 (COVID-19) Nucleocapsid抗体 [HL448]
Brand: GeneTex
SKU: GTX635686
Price: 4000.00 RMB

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL448] used in ELISA (ELISA). GTX635686

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL448] used in Western Blot (WB). GTX635686

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL448] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635686

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL448] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX635686

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL448] used in IHC (Paraffin sections) (IHC-P). GTX635686

Anti-SARS-CoV-2 (COVID-19) Nucleocapsid antibody [HL448] used in Lateral Flow (Lateral Flow). GTX635686

目录号 GTX635686

文献引用 9

宿主	Rabbit
克隆	Monoclonal
克隆编号	HL448
同种型	IgG
实验应用	WB, ICC/IF, IHC-P, ELISA, Lateral Flow, Sandwich ELISA, IHC-P (cell pellet)
种属反应	SARS Coronavirus, SARS Coronavirus 2
产品包装	100 μl (¥4000), 25 μl (¥1700)

查看所有 SARS-CoV-2 (COVID-19) nucleocapsid 的产品

View product citations for antibody GTX635686 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:1000-1:10000
ICC/IF	1:100-1:1000
IHC-P	1:5000-1:10000
ELISA	Assay dependent
Lateral Flow	Assay dependent
Sandwich ELISA	Assay dependent
IHC-P (cell pellet)	Assay dependent
Note : IHC-P Recommended heat-Induced Epitope Retrieval pH 6.0 for 20 minutes and background Eraser (BGE) blocking step. Lateral Flow Capture : GTX635688, Detection : GTX635686 Please notice that the detection antibodies need to be conjugated to Gold to function when paired with capture antibodies. Please contact us for custom Gold-conjugated antibody. Sandwich ELISA Capture : GTX635686 , Detection: GTX635678 / GTX635688. or Capture : GTX635685 / GTX635689 / GTX635679 , Detection: GTX635686-01. Please notice that detection antibodies need to be conjugated to HRP to function when paired with capture antibodies. Please contact us for custom HRP-conjugated antibody.

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

产品说明

This antibody detects both SARS-CoV nucleocapsid and SARS-CoV-2 nucleocapsid proteins. Our internal testing indicates no cross-reactivity with MERS-CoV nucleocapsid protein.Gold conjugation version is available upon request.This antibody is able to detect multiple SARS-CoV-2 VOCs, including Omicron variant.

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

SARS Coronavirus 2

免疫原

Full length SARS-CoV-2 (COVID-19) nucleocapsid Recombinant protein. (SARS-CoV-2 (strain Wuhan-Hu-1))

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

RRID

AB_2888557

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

2019-nCoV Nucleocapsid , SARS-CoV-2 Nucleocapsid , COVID-19 Nucleocapsid , 2019-nCoV NP , SARS-CoV-2 NP , COVID-19 NP

研究领域

產品图片

GTX635686 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag protein (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL249] (GTX635678) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635678 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635686. Please contact us for custom HRP-conjugated antibody.

GTX635686 WB Image

SARS-CoV-2 (COVID-19) nucleocapsid protein, B.1.1.529 / omicron variant, His tag (1 μg, GTX136779-pro) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX635686 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX635686 ICC/IF Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

GTX635686 WB Image

Non-transfected (–) and transfected (+) Vero E6 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX635686 ELISA Image

Sandwich ELISA detection of recombinant full-length Nucleocapsid proteins derived from different strains of SARS-CoV-2 (COVID-19) (Wild type or B.1.617.2 delta variant) using antibodies as below.
Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) (1 μg/mL).

Please notice that GTX635686 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635689. Please contact us for custom HRP-conjugated antibody.

GTX635686 WB Image

SARS-CoV-2 (COVID-19) nucleocapsid protein, B.1.1.529 / Omicron variant, His tag (0.5 μg), expressed by HEK293 cells (GTX136779-pro) or E. coli (GTX03400-pro), were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX635686 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro) (50 ng). Coated protein probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) (4.8*10⁻⁶ ~ 20 nM). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.
EC50 : 0.011 nM

GTX635686 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 (COVID-19) nucleocapsid protein, B.1.1.529 / Omicron variant, His tag (GTX136779-pro) (21.91-0.34 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686), SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689), SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685), SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688), and SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL344] (GTX635679) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

GTX635686 ELISA Image

Sandwich ELISA detection of recombinant nucleocapsid protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; B.1.1.529 Omicron variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) (1 μg/mL)
Please notice that GTX635686 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635689. Please contact us for custom HRP-conjugated antibody.

GTX635686 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635686 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635689. Please contact us for custom HRP-conjugated antibody.

GTX635686 IHC-P (cell pellet) Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block (GTX435641).
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX635686 WB Image

GTX635686 IHC-P Image

SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis of SARS-CoV-2 infected human lung.

The IHC-P was performed by HISTOWIZ.

GTX635686 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant SARS-CoV-2 Nucleocapsid protein (D63G, R203M, D377Y, R385K), His Tag (delta variant) and SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (wild type) (GTX135592-pro) (21.91-0.34 nM). Coated protein was probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) (1 μg/mL). Goat anti-rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

GTX635686 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 Nucleocapsid protein (D63G, R203M, D377Y, R385K), His Tag (delta variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) (1 μg/mL)
Please notice that GTX635688 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635686. Please contact us for custom HRP-conjugated antibody.

GTX635686 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (GTX135592-pro) using SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5410] (GTX635685) as capture antibody at concentration of 5 μg/mL and HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) as detection antibody at concentration of 1 μg/mL.
Please notice that GTX635686 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635685. Please contact us for custom HRP-conjugated antibody.

GTX635686 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (GTX135592-pro) (1000-15.625 ng/mL). Coated protein was probed with SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) (1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) was used to detect bound primary antibody.

GTX635686 ELISA Image

Sandwich ELISA detection of recombinant SARS-CoV-2 (COVID-19) nucleocapsid protein, His tag (GTX135592-pro) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL455] (GTX635688) (1 μg/mL)
Please notice that GTX635688 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635686. Please contact us for custom HRP-conjugated antibody.

GTX635686 IHC-P Image

GTX635686 Lateral Flow Image

Detection of nucleoproteins (NP) derived from SARS-CoV-2 (S), Influenza A virus (A), and Influenza B virus (B) by lateral flow assay using the monoclonal antibody pair. “C” stands for control.

Capture:
S. SARS-CoV-2 (COVID-19) Nucleocapsid antibody (GTX635688 [HL455])
A. Influenza A virus Nucleoprotein antibody (GTX636199 [HL1078])
B. Influenza B virus Nucleoprotein antibody (GTX636194 [HL1073])
Detection:
S. SARS-CoV-2 (COVID-19) Nucleocapsid antibody (GTX635686 [HL448])
A. Influenza A virus Nucleoprotein antibody (GTX637790 [HL1953])
B. Influenza B virus Nucleoprotein antibody (GTX636099 [HL1068])

Samples (40 ng) :
1. Lysis buffer
2. SARS-CoV-2 (COVID-19) Nucleocapsid protein, His tag (GTX135592-pro)
3. Influenza A virus Nucleoprotein (A/California/2009 (H1N1)) (GTX135904-pro)
4. Influenza A virus Nucleoprotein (A/Hong Kong/2671/2019 (H3N2)) (GTX136317-pro)
5. Influenza B virus Nucleoprotein (B/Sydney/3/2004), DDDDK Tag (GTX135867-pro)
6. Influenza B virus Nucleoprotein (B/Victoria/02/1987), DDDDK Tag (GTX136277-pro)
7. Mixture of SARS-CoV-2 (GTX135592-pro), Flu A-H1N1 (GTX135904-pro), and Flu B Nucleoprotein (GTX135867-pro)

GTX635686 ELISA Image

Sandwich ELISA detection of recombinant nucleocapsid protein(s) derived from different strains of SARS-CoV-2 virus (ie., Wild type; BF.7 Omicron variant; BQ.1 Omicron variant) using antibodies as below.

Capture: SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL5511] (GTX635689) (5 μg/mL)
Detection: HRP-conjugated SARS-CoV-2 (COVID-19) nucleocapsid antibody [HL448] (GTX635686) (1 μg/mL)
Please notice that GTX635686 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635689. Please contact us for custom HRP-conjugated antibody.

文献引用

Application Reference

Ardanuy J et al. mBio 2023; 14 (5) : e0158723 Pyronaridine tetraphosphate is an efficacious antiviral and anti-inflammatory active against multiple highly pathogenic coronaviruses.

Arostegui D et al. JPGN Rep 2022; 3 (1) : e152 Persistent SARS-CoV-2 Nucleocapsid Protein Presence in the Intestinal Epithelium of a Pediatric Patient 3 Months After Acute Infection.

Zhu C et al. Nat Commun 2022; 13 (1) : 4503 An intranasal ASO therapeutic targeting SARS-CoV-2.

实验应用 : IHC-P

Bullock HA et al. Front Med (Lausanne) 2022; 9 : 1099408 Probable vertical transmission of Alpha variant of concern (B.1.1.7) with evidence of SARS-CoV-2 infection in the syncytiotrophoblast, a case report.

实验应用 : IHC-P

Sarah Reagan-Steiner et al. Emerg Infect Dis 2022; 28 (3) : 510-517 Detection of SARS-CoV-2 in Neonatal Autopsy Tissues and Placenta.

实验应用 : IHC-P

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Application Reference

Ardanuy J et al. mBio 2023; 14 (5):e0158723 Pyronaridine tetraphosphate is an efficacious antiviral and anti-inflammatory active against multiple highly pathogenic coronaviruses.

Arostegui D et al. JPGN Rep 2022; 3 (1):e152 Persistent SARS-CoV-2 Nucleocapsid Protein Presence in the Intestinal Epithelium of a Pediatric Patient 3 Months After Acute Infection.

Zhu C et al. Nat Commun 2022; 13 (1):4503 An intranasal ASO therapeutic targeting SARS-CoV-2.

实验应用 : IHC-P

Bullock HA et al. Front Med (Lausanne) 2022; 9:1099408 Probable vertical transmission of Alpha variant of concern (B.1.1.7) with evidence of SARS-CoV-2 infection in the syncytiotrophoblast, a case report.

实验应用 : IHC-P

Rajendra Karki et al. Sci Immunol 2022;:eabo6294 ZBP1-dependent inflammatory cell death, PANoptosis, and cytokine storm disrupt IFN therapeutic efficacy during coronavirus infection.

实验应用 : IHC-P

Mostafa M Eltobgy et al. Proc Natl Acad Sci U S A 2022; 119 (21):e2202012119 Caspase-4/11 exacerbates disease severity in SARS-CoV-2 infection by promoting inflammation and immunothrombosis.

实验应用 : IHC-P

Sarah Reagan-Steiner et al. Emerg Infect Dis 2022; 28 (3):510-517 Detection of SARS-CoV-2 in Neonatal Autopsy Tissues and Placenta.

实验应用 : IHC-P

Jiran Li et al. ACS Sens 2021; 6 (3):1270-1278 Microfluidic Magneto Immunosensor for Rapid, High Sensitivity Measurements of SARS-CoV-2 Nucleocapsid Protein in Serum.

Vazquez C et al. PLoS One 2021; 16 (6):e0253089 SARS-CoV-2 viral proteins NSP1 and NSP13 inhibit interferon activation through distinct mechanisms.

实验应用 : WB, ICC/IF

SDS
PBS.pdf

产品包装	报价 (¥)
100 μl	¥ 4000
25 μl	¥ 1700
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SARS-CoV-2 (COVID-19) Nucleocapsid抗体 [HL448]

目录号 GTX635686

宿主

克隆

克隆编号

同种型

实验应用

种属反应

产品应用

应用说明

产品说明

产品属性

形式

存储溶液

存放说明

浓度

免疫原种属

免疫原

纯化方式

偶联

RRID

注意事项

相关讯息

别名

研究领域

產品图片

GTX635686 ELISA Image

GTX635686 WB Image

GTX635686 WB Image

GTX635686 ICC/IF Image

GTX635686 WB Image

GTX635686 ELISA Image

GTX635686 WB Image

GTX635686 ELISA Image

GTX635686 ELISA Image

GTX635686 ELISA Image

GTX635686 ELISA Image

GTX635686 IHC-P (cell pellet) Image

GTX635686 WB Image

GTX635686 IHC-P Image

GTX635686 ELISA Image

GTX635686 ELISA Image

GTX635686 ELISA Image

GTX635686 ELISA Image

GTX635686 ELISA Image

GTX635686 IHC-P Image

GTX635686 Lateral Flow Image

GTX635686 ELISA Image

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