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SARS-CoV / SARS-CoV-2 (COVID-19) nsp8抗体 [5A10]

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nsp8 antibody [5A10] used in Western Blot (WB). GTX632696
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nsp8 antibody [5A10] used in Flow cytometry (FACS). GTX632696
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nsp8 antibody [5A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632696
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nsp8 antibody [5A10] used in Western Blot (WB). GTX632696
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nsp8 antibody [5A10] used in Western Blot (WB). GTX632696

目录号 GTX632696

文献引用 9
2

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 5A10

同种型

 IgG1

实验应用

 WB, ICC/IF, IHC-Fr, FACS

种属反应

 SARS Coronavirus, SARS Coronavirus 2
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 SARS-CoV-2 (COVID-19) nsp8 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-Fr Assay dependent
FACS Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

阳性对照

NSP8-transfected 293T

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.67 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

SARS Coronavirus

免疫原

The immunogen used to generate this antibody corresponds to SARS virus nsp8.

纯化方式

Affinity purified by Protein G.

偶联

Unconjugated

RRID

AB_2888329

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

2019-nCoV nsp8 , SARS-coV-2 nsp8 , COVID-19 nsp8

研究领域

產品图片

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nsp8 antibody [5A10] used in Western Blot (WB). GTX632696

GTX632696 WB Image

Non-infected (–) and infected (+, 48h pl MOI 0.01) whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (GTX632696) diluted at 1:1000.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nsp8 antibody [5A10] used in Flow cytometry (FACS). GTX632696

GTX632696 FACS Image

SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (GTX632696) detects SARS-CoV-2 (COVID-19) NSP8 by flow cytometry analysis.
Sample: Vero E6 cells infected with SARS-CoV-2.
Black: Uninfected Vero E6 cells was used as a control.
Red: SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (GTX632696) dilution: 1:100.
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nsp8 antibody [5A10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632696

GTX632696 ICC/IF Image

SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] detects SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 stained by SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (GTX632696) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nsp8 antibody [5A10] used in Western Blot (WB). GTX632696

GTX632696 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) NSP8 antibody [5A10] (GTX632696) diluted at 1:5000.

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nsp8 antibody [5A10] used in Western Blot (WB). GTX632696

GTX632696 WB Image

The data was published in the 2020 in Cell Death Dis. PMID: 32814759

文献引用

Application Reference

Zhang X et al. PLoS Pathog 2023; 19 (11) : e1011792 SARS-CoV-2 Nsp8 suppresses MDA5 antiviral immune responses by impairing TRIM4-mediated K63-linked polyubiquitination
实验应用 : WB
A Guichard et al. Cell Rep 2023; 42 (8) : 112842 A comprehensive Drosophila resource to identify key functional interactions between SARS-CoV-2 factors and host proteins
实验应用 : WB
Hirano M et al. Nat Biotechnol 2022; 40 (7) : 1132-1142 A highly photostable and bright green fluorescent protein.
Patrick Madden et al. Res Sq 2022; An immunoPET probe to SARS-CoV-2 reveals early infection of the male genital tract in rhesus macaques.
实验应用 : IHC-Fr
Madden PJ et al. bioRxiv 2022; An immunoPET probe to SARS-CoV-2 reveals early infection of the male genital tract in rhesus macaques.
实验应用 : IHC-Fr
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产品评论

SARS-CoV / SARS-CoV-2 (COVID-19) nsp8 antibody [5A10] 目录号 GTX632696

Rating
( Average 5 based on 2 users reviews)
实验应用
Western Blot(WB)
( Average 5 based on 1 users reviews)
Flow cytometry(FACS)
( Average 5 based on 1 users reviews)
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nsp8 antibody [5A10] used in Flow cytometry (FACS). GTX632696
Date : Anonymous submitted on 14-Jul-2020
Score :
Application Tested : FACS
Sample Species : SARS-CoV-2
Sample : Vero E6 cells infected with SARS-CoV-2 USA-WA1/2020
Fixation : 4% formaldehyde in PBS
Permeabilization /Antigen retrieval : BD Cytofix/Cytoperm
Primary Antibody : 1:100, 25°C, 0.5Hr
Anti-SARS-CoV / SARS-CoV-2 (COVID-19) nsp8 antibody [5A10] used in Western Blot (WB). GTX632696
Date : Anonymous submitted on 12-Mar-2020
Score :
Application Tested : WB
Sample Species : SARS-CoV, SARS-CoV-2
Primary Antibody : 1:1000, 4°C, 16Hr
我要评论产品
SDS
PBS.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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