SARS-CoV / SARS-CoV-2 (COVID-19) Nucleocapsid抗体 [6H3]

Name: SARS-CoV / SARS-CoV-2 (COVID-19) Nucleocapsid抗体 [6H3]
Brand: GeneTex
SKU: GTX632269
Price: 4000.00 RMB
Rating: 5 (1 reviews)

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) Nucleocapsid antibody [6H3] used in ELISA (ELISA). GTX632269

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) Nucleocapsid antibody [6H3] used in Western Blot (WB). GTX632269

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) Nucleocapsid antibody [6H3] used in Immunoprecipitation (IP). GTX632269

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) Nucleocapsid antibody [6H3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX632269

Anti-SARS-CoV / SARS-CoV-2 (COVID-19) Nucleocapsid antibody [6H3] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX632269

目录号 GTX632269

文献引用 43

宿主	Mouse
克隆	Monoclonal
克隆编号	6H3
同种型	IgG1
实验应用	WB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA, Sandwich ELISA, IHC-P (cell pellet)
种属反应	SARS Coronavirus, SARS Coronavirus 2
产品包装	100 μl (¥4000), 25 μl (¥1700)

查看所有 SARS-CoV-2 (COVID-19) nucleocapsid 的产品

View product citations for antibody GTX632269 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:1000-1:10000
ICC/IF	Assay dependent
IHC-P	Assay dependent
IHC-Fr	Assay dependent
IP	Assay dependent
ELISA	Assay dependent
Sandwich ELISA	Assay dependent
IHC-P (cell pellet)	Assay dependent
Note : Sandwich ELISA Capture : GTX632269 / GTX135361 / GTX135357, Detection : GTX135357 / GTX632269

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

产品说明

This antibody detects both SARS-CoV nucleocapsid and SARS-CoV-2 nucleocapsid proteins. Our internal testing indicates no cross-reactivity with MERS-CoV nucleocapsid protein.

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.5 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

SARS Coronavirus

免疫原

The immunogen used to generate this antibody corresponds to SARS-CoV Nucleocapsid (121-422 a.a.).

纯化方式

Affinity purified by Protein G.

偶联

Unconjugated

RRID

AB_2888304

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

2019-nCoV Nucleocapsid , SARS-CoV-2 Nucleocapsid , COVID-19 Nucleocapsid , 2019-nCoV NP , SARS-CoV-2 NP , COVID-19 NP

研究领域

產品图片

GTX632269 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135357-pro) using GTX135357 as capture antibody at concentration of 5 μg/mL and GTX632269 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX632269 WB Image

Non-infected (–) and infected (+, 48h pl MOI 0.01) Caco2 whole cell extracts were separated by SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:1000.

GTX632269 IP Image

Immunoprecipitation of SARS-CoV-2 NP transfected 293T whole cell extracts using 2 μg of SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269).
Western blot analysis was performed using SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269).
EasyBlot HRP-conjugated anti mouse IgG antibody (GTX221667-01) was used to detect the primary antibody.

GTX632269 WB Image

SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135592-pro, 0.5 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:5000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX632269 ELISA Image

Sandwich ELISA detection of non-transfected (GTX535673) and SARS-CoV-2 nucleocapsid (full length) transfected (GTX535665) 293T whole cell extracts using SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) as capture antibody at concentration of 5 μg/mL and SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX632269 ELISA Image

Sandwich ELISA detection of recombinant full-length SARS-CoV-2 (COVID-19) nucleocapsid protein (GTX135357-pro) using GTX135361 as capture antibody at concentration of 5 μg/mL and GTX632269 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

GTX632269 ICC/IF Image

ICC/IF analysis of VeroE6 cells over-expressing SARS-CoV Nucleocapsid protein using GTX632269 SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3].

GTX632269 IHC-P (cell pellet) Image

SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] detects SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis.
Sample: Mock (GTX435670) and SARS-CoV-2 (COVID-19) Nucleocapsid transfected 293T cell FFPE Cell Pellet Block (GTX435641).
Red: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX632269 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

GTX632269 ICC/IF Image

SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] detects SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

GTX632269 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:5000. The HRP-conjugated anti-mouset IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX632269 IHC-P (cell pellet) Image

SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] detects SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid protein by immunohistochemical analysis.
Sample: Paraffin-embedded SARS-CoV-2 (COVID-19) Nucleocapsid FFPE Cell Pellet Block.
Red: SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:1000.
Green: SARS-CoV-2 (COVID-19) nucleocapsid stained by SARS-CoV-2 (COVID-19) nucleocapsid antibody (GTX135357) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX632269 WB Image

WB analysis of SARS-CoV infected Vero E6 cells harvested at different time-points post infection (T = 0, 8, 16 and 24 h) using GTX632269 SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3].

GTX632269 WB Image

Non-transfected (–) and transfected (+) Vero E6 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SARS-CoV / SARS-CoV-2 (COVID-19) nucleocapsid antibody [6H3] (GTX632269) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

文献引用

Application Reference

W-F Tang et al. Antimicrob Agents Chemother 2024; : e0095623 BPR3P0128, a non-nucleoside RNA-dependent RNA polymerase inhibitor, inhibits SARS-CoV-2 variants of concern and exerts synergistic antiviral activity in combination with remdesivir

实验应用 : WB

Asano R et al. Cureus 2023; 15 (12) : e51049 Three Types of Demyelination, Perivenous, Confluent, and Perineuronal Nets-Rich in a COVID-19 Patient With Meningoencephalomyelitis.

R Asano et al. Cureus 2023; Three Types of Demyelination, Perivenous, Confluent, and Perineuronal Nets-Rich in a COVID-19 Patient With Meningoencephalomyelitis

实验应用 : IHC-P

CF Yang et al. J Biomed Sci 2023; Human ACE2 protein is a molecular switch controlling the mode of SARS-CoV-2 transmission

Ma J et al. Pharm Biol 2023; 61 (1) : 1446-1453 Extracts of Thesium chinense inhibit SARS-CoV-2 and inflammation in?vitro.

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产品评论

SARS-CoV / SARS-CoV-2 (COVID-19) Nucleocapsid antibody [6H3] 目录号 GTX632269

Rating

( Average 5 based on 1 users reviews)

实验应用

Western Blot(WB)

( Average 5 based on 1 users reviews)

Date : Anonymous submitted on 05-Mar-2020

Score :

Application Tested : WB

Sample Species : SARS-CoV

Sample : Caco2 cells

Primary Antibody : 1:1000, 4°C, 16Hr

我要评论产品

Application Reference

W-F Tang et al. Antimicrob Agents Chemother 2024;:e0095623 BPR3P0128, a non-nucleoside RNA-dependent RNA polymerase inhibitor, inhibits SARS-CoV-2 variants of concern and exerts synergistic antiviral activity in combination with remdesivir

实验应用 : WB

Asano R et al. Cureus 2023; 15 (12):e51049 Three Types of Demyelination, Perivenous, Confluent, and Perineuronal Nets-Rich in a COVID-19 Patient With Meningoencephalomyelitis.

R Asano et al. Cureus 2023; Three Types of Demyelination, Perivenous, Confluent, and Perineuronal Nets-Rich in a COVID-19 Patient With Meningoencephalomyelitis

实验应用 : IHC-P

CF Yang et al. J Biomed Sci 2023; Human ACE2 protein is a molecular switch controlling the mode of SARS-CoV-2 transmission

Ma J et al. Pharm Biol 2023; 61 (1):1446-1453 Extracts of Thesium chinense inhibit SARS-CoV-2 and inflammation in?vitro.

Swain J et al. iScience 2023; 26 (8):107384 F-actin nanostructures rearrangements and regulation are essential for SARS-CoV-2 particle production in host pulmonary cells.

McMahon A et al. PLoS Pathog 2023; 19 (6):e1011484 High-throughput super-resolution analysis of influenza virus pleomorphism reveals insights into viral spatial organization.

Carrell C et al. Anal Methods 2023; 15 (22):2721-2728 Capillary driven microfluidic sequential flow device for point-of-need ELISA: COVID-19 serology testing.

Williams DM et al. iScience 2023; 26 (7):107056 Establishing SARS-CoV-2 membrane protein-specific antibodies as a valuable serological target via high-content microscopy.

Hou P et al. Autophagy 2023; 19 (2):551-569 The ORF7a protein of SARS-CoV-2 initiates autophagy and limits autophagosome-lysosome fusion via degradation of SNAP29 to promote virus replication.

Tamai K et al. Cell Rep Methods 2022; 2 (10):100314 iPSC-derived mesenchymal cells that support alveolar organoid development.

实验应用 : ICC/IF

Kumar CS et al. ACS Infect Dis 2022; 8 (10):2119-2132 Virus-Like Particles of SARS-CoV-2 as Virus Surrogates: Morphology, Immunogenicity, and Internalization in Neuronal Cells.

Yeh CT et al. Biomedicines 2022; 10 (11) Immunoglobulin Y Specific for SARS-CoV-2 Spike Protein Subunits Effectively Neutralizes SARS-CoV-2 Infectivity and Ameliorates Disease Manifestations In Vivo.

Pin-Hsuan Chen et al. Sens Actuators B Chem 2022; 357:131415 Saliva-based COVID-19 detection: A rapid antigen test of SARS-CoV-2 nucleocapsid protein using an electrical-double-layer gated field-effect transistor-based biosensing system.

Yasuo Ariumi et al. J Virol 2022; 96 (6):e0000222 Host Cellular RNA Helicases Regulate SARS-CoV-2 Infection.

实验应用 : WB, ICC/IF, IP

Patrick Madden et al. Res Sq 2022; An immunoPET probe to SARS-CoV-2 reveals early infection of the male genital tract in rhesus macaques.

实验应用 : IHC-Fr

Madden PJ et al. bioRxiv 2022; An immunoPET probe to SARS-CoV-2 reveals early infection of the male genital tract in rhesus macaques.

实验应用 : IHC-Fr

Magalh?es AC et al. Pathogens 2022; 11 (3) InfectionCMA: A Cell MicroArray Approach for Efficient Biomarker Screening in In Vitro Infection Assays.

实验应用 : IHC-P

Martin MDGM et al. Insights Imaging 2022; 13 (1):7 Postmortem brain 7T MRI with minimally invasive pathological correlation in deceased COVID-19 subjects.

Wen-Fang Tang et al. Biomed J 2021; 44 (3):293-303 Perilla (Perilla frutescens) leaf extract inhibits SARS-CoV-2 via direct virus inactivation.

实验应用 : WB

S?bastien Lyonnais et al. Sci Rep 2021; 11 (1):11885 Atomic force microscopy analysis of native infectious and inactivated SARS-CoV-2 virions.

实验应用 : WB

Keiji Tanimoto et al. Sci Rep 2021; 11 (1):16629 Inhibiting SARS-CoV-2 infection in vitro by suppressing its receptor, angiotensin-converting enzyme 2, via aryl-hydrocarbon receptor signal.

实验应用 : WB

Takeshi Yamamotoya et al. Sci Rep 2021; 11 (1):18581 Prolyl isomerase Pin1 plays an essential role in SARS-CoV-2 proliferation, indicating its possibility as a novel therapeutic target.

实验应用 : WB

Duarte-Neto AN et al. Clin Infect Dis 2021; 73 (Suppl_5):S442-S453 Ultrasound-Guided Minimally Invasive Tissue Sampling: A Minimally Invasive Autopsy Strategy During the COVID-19 Pandemic in Brazil, 2020.

Serra AM et al. Braz J Infect Dis 2021; 25 (6):101651 SARS-CoV-2 identification in an acute appendicitis case: Acute abdomen as manifestation of Multisystem Inflammatory Syndrome in a child with COVID-19.

实验应用 : IHC-P

Li X et al. Cell Mol Immunol 2021; SARS-CoV-2 ORF10 suppresses the antiviral innate immune response by degrading MAVS through mitophagy

实验应用 : WB

Samper IC et al. ACS Sens 2021; 6 (11):4067-4075 Electrochemical Capillary-Flow Immunoassay for Detecting Anti-SARS-CoV-2 Nucleocapsid Protein Antibodies at the Point of Care.

Kim DH et al. Sci Rep 2021; 11 (1):21462 Hemin as a novel candidate for treating COVID-19 via heme oxygenase-1 induction.

实验应用 : WB

Duarte-Neto AN et al. Andrology 2021; (Epub) Testicular pathology in fatal COVID-19: A descriptive autopsy study.

实验应用 : IHC-P

Gopal V et al. ACS Appl Mater Interfaces 2021; 13 (26):30317-30325 Zinc-Embedded Polyamide Fabrics Inactivate SARS-CoV-2 and Influenza A Virus.

实验应用 : WB

Sasaki M. et al. PLoS Pathog. 2021; 17 (1):e1009233 SARS-CoV-2 variants with mutations at the S1/S2 cleavage site are generated in vitro during propagation in TMPRSS2-deficient cells.

实验应用 : WB

Matsuura R et al. Viruses 2021; 13 (5) SARS-CoV-2 Disinfection of Air and Surface Contamination by TiO₂ Photocatalyst-Mediated Damage to Viral Morphology, RNA, and Protein.

实验应用 : WB

Ciccosanti F et al. Antiviral Res 2021; 190:105064 Proteomic analysis identifies the RNA helicase DDX3X as a host target against SARS-CoV-2 infection.

实验应用 : WB, ICC/IF

Mou H et al. PLoS Pathog 2021; 17 (4):e1009501 Mutations derived from horseshoe bat ACE2 orthologs enhance ACE2-Fc neutralization of SARS-CoV-2.

实验应用 : ICC/IF

Di Domenico M et al. Diagnostics (Basel) 2021; 11 (4) Detection of SARS-COV-2 Proteins Using an ELISA Test.

Duarte-Neto AN et al. EClinicalMedicine 2021; 35:100850 An autopsy study of the spectrum of severe COVID-19 in children: From SARS to different phenotypes of MIS-C.

实验应用 : IHC-P

Tang W.F. et al. Biomedical Journal 2021; (Epub) Perilla (Perilla frutescens) leaf extract inhibits SARS-CoV-2 via direct virus inactivation.

实验应用 : WB

Li J et al. ACS Sens 2021; 6 (3):1270-1278 Microfluidic Magneto Immunosensor for Rapid, High Sensitivity Measurements of SARS-CoV-2 Nucleocapsid Protein in Serum.

Tanimoto K. et al. bioRxiv 2021; (Epub) Inhibiting SARS-CoV-2 infection in vitro by suppressing its receptor, angiotensin-converting enzyme 2, via aryl-hydrocarbon receptor signal.

实验应用 : WB

Moustaqil M et al. Emerg Microbes Infect 2021; 10 (1):178-195 SARS-CoV-2 proteases PLpro and 3CLpro cleave IRF3 and critical modulators of inflammatory pathways (NLRP12 and TAB1): implications for disease presentation across species.

实验应用 : WB

Kotaki T et al. Sci Rep 2021; 11 (1):2229 A PCR amplicon-based SARS-CoV-2 replicon for antiviral evaluation.

实验应用 : ICC/IF

Gopal V et al. bioRxiv 2020; (Epub) Zinc-embedded fabrics inactivate SARS-CoV-2 and influenza A virus.

实验应用 : WB

Zhang Y et al. Antiviral Res 2020; 185:104974 A bacterial artificial chromosome (BAC)-vectored noninfectious replicon of SARS-CoV-2.

实验应用 : WB

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产品包装	报价 (¥)
100 μl	¥ 4000
25 μl	¥ 1700
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SARS-CoV / SARS-CoV-2 (COVID-19) Nucleocapsid抗体 [6H3]

目录号 GTX632269

宿主

克隆

克隆编号

同种型

实验应用

种属反应

产品应用

应用说明

产品说明

产品属性

形式

存储溶液

存放说明

浓度

免疫原种属

免疫原

纯化方式

偶联

RRID

注意事项

相关讯息

别名

研究领域

產品图片

GTX632269 ELISA Image

GTX632269 WB Image

GTX632269 IP Image

GTX632269 WB Image

GTX632269 ELISA Image

GTX632269 ELISA Image

GTX632269 ICC/IF Image

GTX632269 IHC-P (cell pellet) Image

GTX632269 WB Image

GTX632269 ICC/IF Image

GTX632269 WB Image

GTX632269 IHC-P (cell pellet) Image

GTX632269 WB Image

GTX632269 WB Image

文献引用

Application Reference

产品评论

SARS-CoV / SARS-CoV-2 (COVID-19) Nucleocapsid antibody [6H3] 目录号 GTX632269

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