GeneTex中国官方网站
  • China

SCGN抗体 [N2C3]

Anti-SCGN antibody [N2C3] used in IHC (Frozen sections) (IHC-Fr). GTX115443
Anti-SCGN antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115443
Anti-SCGN antibody [N2C3] used in Western Blot (WB). GTX115443
Anti-SCGN antibody [N2C3] used in IHC (Frozen sections) (IHC-Fr). GTX115443
Anti-SCGN antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX115443
Anti-SCGN antibody [N2C3] used in Western Blot (WB). GTX115443
Anti-SCGN antibody [N2C3] used in IHC (Frozen sections) (IHC-Fr). GTX115443

目录号 GTX115443

1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC-Fr

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 SCGN 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

32 kDa. ( Note )

阳性对照

HeLa , mouse brain , rat brain

预测种属反应

Zebrafish, Bovine, Pig, Xenopus tropicalis(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human SCGN. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_10719884

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

secretagogin, EF-hand calcium binding protein , CALBL , DJ501N12.8 , SECRET , SEGN , setagin

细胞定位

Cytoplasm , Secreted , Cytoplasmic vesicle , secretory vesicle membrane; Peripheral membrane protein; Cytoplasmic side

功能与背景

The encoded protein is a secreted calcium-binding protein which is found in the cytoplasm. It is related to calbindin D-28K and calretinin. This protein is thought to be involved in KCL-stimulated calcium flux and cell proliferation. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-SCGN antibody [N2C3] used in IHC (Frozen sections) (IHC-Fr). GTX115443

GTX115443 IHC-Fr Image

SCGN antibody [N2C3] detects SCGN protein expression by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green:SCGN protein stained by SCGN antibody [N2C3] (GTX115443) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-SCGN antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115443

GTX115443 ICC/IF Image

SCGN antibody [N2C3] detects SCGN protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SCGN stained by SCGN antibody [N2C3] (GTX115443) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SCGN antibody [N2C3] used in Western Blot (WB). GTX115443

GTX115443 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SCGN antibody [N2C3] (GTX115443) diluted at 1:500.

Anti-SCGN antibody [N2C3] used in IHC (Frozen sections) (IHC-Fr). GTX115443

GTX115443 IHC-Fr Image

SCGN antibody [N2C3] detects SCGN protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: SCGN protein stained by SCGN antibody [N2C3] (GTX115443) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-SCGN antibody [N2C3] used in IHC (Paraffin sections) (IHC-P). GTX115443

GTX115443 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using SCGN(GTX115443) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SCGN antibody [N2C3] used in Western Blot (WB). GTX115443

GTX115443 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
12% SDS PAGE
GTX115443 diluted at 1:1000

Anti-SCGN antibody [N2C3] used in IHC (Frozen sections) (IHC-Fr). GTX115443

GTX115443 IHC-Fr Image

SCGN antibody [N2C3] detects SCGN Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: SCGN stained by SCGN antibody [N2C3] (GTX115443) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

文献引用

目前没有使用 SCGN antibody [N2C3] (GTX115443) 的文献被收录。快来回报我们第一篇参考文献吧！
我要回报文献

产品评论

SCGN antibody [N2C3] 目录号 GTX115443

Rating
( Average 5 based on 1 users reviews)
实验应用
IHC (Frozen sections)(IHC-Fr)
( Average 5 based on 1 users reviews)
Anti-SCGN antibody [N2C3] used in IHC (Frozen sections) (IHC-Fr). GTX115443
Date : Anonymous submitted on 15-Dec-2020
Score :
Application Tested : IHC-Fr
Sample Species : Ms
Sample : Olfactory bulb, free floating section
Blocking : 1% BSA/PBS, 25°C, 0.5Hr
Fixation : 4% PFA/PBS
Permeabilization /Antigen retrieval : 0.3% Triton X-100
Primary Antibody : 1:500, 4°C, 16Hr
我要评论产品
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
联系经销商
欣博盛生物科技有限公司
欣博盛生物科技有限公司
(全国热线) 4006-800-892 / (深圳) 0755-26755892 / (北京) 010-88594029 / (上海) 021-34613729 / (广州) 18024516375 / (香港) +852-69410778
http://www.neobioscience.com
genetex@neobioscience.com