产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
293T
预测种属反应
Zebrafish, Drosophila, Bovine, Chicken, Xenopus tropicalis(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the N-terminus region of human SDHA. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein G.
偶联
Unconjugated
RRID
AB_2888322
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
succinate dehydrogenase complex flavoprotein subunit A , CMD1GG , FP , PGL5 , SDH1 , SDH2 , SDHF
细胞定位
Mitochondrion inner membrane
功能与背景
This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29. [provided by RefSeq]
数据库
研究领域
產品图片
|
GTX632636 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (GTX632636) diluted at 1:1000.
|
|
GTX632636 WB Image
HepG2 and mitochondria extracts (30 μg) were separated by SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (GTX632636) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
|
|
GTX632636 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (GTX632636) diluted at 1:3000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
|
|
GTX632636 IHC-P Image
SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.SDHA stained by SDHA antibody [GT20710] (GTX632636) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX632636 IHC-P Image
SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded human esophageal carcinoma.SDHA stained by SDHA antibody [GT20710] (GTX632636) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX632636 IHC-P Image
SDHA antibody [GT20710] detects SDHA protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.SDHA stained by SDHA antibody [GT20710] (GTX632636) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX632636 ICC/IF Image
SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunofluorescent analysis.Sample: Mock and treated HeLa cells were fixed in ice-cold MeOH for 5 min.Green: SDHA stained by SDHA antibody [GT20710] (GTX632636) diluted at 1:500.Red: MitoTracker® Red CMXRos, a mitochondria marker.Blue: Fluoroshield with DAPI (GTX30920).
|
|
GTX632636 IHC-P Image
SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.SDHA stained by SDHA antibody [GT20710] (GTX632636) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX632636 IHC-P Image
SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded mouse liver.SDHA stained by SDHA antibody [GT20710] (GTX632636) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
GTX632636 ICC/IF Image
SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: SDHA protein stained by SDHA antibody [GT20710] (GTX632636) diluted at 1:500. Red: Phalloidin, a cytoskeleton marker, diluted at 1:100. Blue: Hoechst 33342 staining.
|
|
GTX632636 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (GTX632636) diluted at 1:3000.
|
|
GTX632636 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SDHA antibody [GT20710] (GTX632636) diluted at 1:3000.
|
|
GTX632636 IHC-P Image
SDHA antibody [GT20710] detects SDHA protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded rat colon.SDHA stained by SDHA antibody [GT20710] (GTX632636) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
文献引用
产品评论
目前没有使用 SDHA antibody [GT20710] (GTX632636) 的产品评论被收录。快发表第一篇产品评论吧!