产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fragment of human SDHB.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
succinate dehydrogenase complex iron sulfur subunit B , CWS2 , IP , PGL4 , SDH , SDH1 , SDH2 , SDHIP
功能与背景
Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
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GTX638300 IHC-P Image
SDHB antibody [HL2251] detects SDHB protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.SDHB stained by SDHB antibody [HL2251] (GTX638300) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638300 IHC-P Image
SDHB antibody [HL2251] detects SDHB protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.SDHB stained by SDHB antibody [HL2251] (GTX638300) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638300 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SDHB antibody [HL2251] (GTX638300) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638300 IHC-P Image
SDHB antibody [HL2251] detects SDHB protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded dog brain.SDHB stained by SDHB antibody [HL2251] (GTX638300) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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