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SDHB抗体

Anti-SDHB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113833
Anti-SDHB antibody used in Western Blot (WB). GTX113833
Anti-SDHB antibody used in IHC (Paraffin sections) (IHC-P). GTX113833
Anti-SDHB antibody used in IHC (Paraffin sections) (IHC-P). GTX113833
Anti-SDHB antibody used in IHC (Paraffin sections) (IHC-P). GTX113833
Anti-SDHB antibody used in IHC (Paraffin sections) (IHC-P). GTX113833
Anti-SDHB antibody used in IHC (Paraffin sections) (IHC-P). GTX113833
Anti-SDHB antibody used in IHC (Paraffin sections) (IHC-P). GTX113833
Anti-SDHB antibody used in Western Blot (WB). GTX113833

目录号 GTX113833

文献引用 8

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 SDHB 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

32 kDa. ( Note )

阳性对照

Mouse brain

预测种属反应

Zebrafish, Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.42 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human SDHB. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2037951

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

succinate dehydrogenase complex iron sulfur subunit B , CWS2 , IP , PGL4 , SDH , SDH1 , SDH2 , SDHIP

细胞定位

Mitochondrion inner membrane

功能与背景

Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-SDHB antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113833

GTX113833 ICC/IF Image

SDHB antibody detects SDHB protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in MeOH for 5 min.
Green: SDHB protein stained by SDHB antibody (GTX113833) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-SDHB antibody used in Western Blot (WB). GTX113833

GTX113833 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SDHB antibody (GTX113833) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SDHB antibody used in IHC (Paraffin sections) (IHC-P). GTX113833

GTX113833 IHC-P Image

SDHB antibody detects SDHB protein at mitochondria on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
SDHB antibody (GTX113833) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SDHB antibody used in IHC (Paraffin sections) (IHC-P). GTX113833

GTX113833 IHC-P Image

SDHB antibody detects SDHB protein at mitochondria on mouse intestine by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
SDHB antibody (GTX113833) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SDHB antibody used in IHC (Paraffin sections) (IHC-P). GTX113833

GTX113833 IHC-P Image

SDHB antibody detects SDHB protein at mitochondria on human ovarian carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian carcinoma.
SDHB antibody (GTX113833) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SDHB antibody used in IHC (Paraffin sections) (IHC-P). GTX113833

GTX113833 IHC-P Image

SDHB antibody detects SDHB protein at mitochondria on mouse stomach by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
SDHB antibody (GTX113833) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SDHB antibody used in IHC (Paraffin sections) (IHC-P). GTX113833

GTX113833 IHC-P Image

SDHB antibody detects SDHB protein at mitochondria on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
SDHB antibody (GTX113833) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SDHB antibody used in IHC (Paraffin sections) (IHC-P). GTX113833

GTX113833 IHC-P Image

SDHB antibody detects SDHB protein at mitochondria on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
SDHB antibody (GTX113833) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SDHB antibody used in Western Blot (WB). GTX113833

GTX113833 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SDHB antibody (GTX113833) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

文献引用

Application Reference

Lee H et al. Nat Commun 2023; 14 (1) : 6721 The mitochondrial fusion protein OPA1 is dispensable in the liver and its absence induces mitohormesis to protect liver from drug-induced injury.
Mingming Zhao et al. Nat Commun 2022; 13 (1) : 1757 Gut microbiota production of trimethyl-5-aminovaleric acid reduces fatty acid oxidation and accelerates cardiac hypertrophy.
实验应用 : WB
种属反应 : Mouse
Lee H et al. J Biol Chem 2020; 295 (19) : 6543-6560 The short variant of optic atrophy 1 (OPA1) improves cell survival under oxidative stress.
Siew JJ et al. Nat Commun 2019; 10 (1) : 3473 Galectin-3 is required for the microglia-mediated brain inflammation in a model of Huntington's disease.
实验应用 : ICC/IF
种属反应 : Mouse
Min HY et al. Cell Death Dis 2019; 10 (11) : 810 The natural compound gracillin exerts potent antitumor activity by targeting mitochondrial complex II.
实验应用 : WB
种属反应 : Human
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Application Reference
Lee H et al. Nat Commun 2023; 14 (1):6721 The mitochondrial fusion protein OPA1 is dispensable in the liver and its absence induces mitohormesis to protect liver from drug-induced injury.
Mingming Zhao et al. Nat Commun 2022; 13 (1):1757 Gut microbiota production of trimethyl-5-aminovaleric acid reduces fatty acid oxidation and accelerates cardiac hypertrophy.
实验应用 : WB
种属反应 : Mouse
Lee H et al. J Biol Chem 2020; 295 (19):6543-6560 The short variant of optic atrophy 1 (OPA1) improves cell survival under oxidative stress.
Siew JJ et al. Nat Commun 2019; 10 (1):3473 Galectin-3 is required for the microglia-mediated brain inflammation in a model of Huntington's disease.
实验应用 : ICC/IF
种属反应 : Mouse
Min HY et al. Cell Death Dis 2019; 10 (11):810 The natural compound gracillin exerts potent antitumor activity by targeting mitochondrial complex II.
实验应用 : WB
种属反应 : Human
Tseng CW et al. Cancer Res 2018; (Epub) Transketolase regulates the metabolic switch to control breast cancer cell metastasis via the alpha-ketoglutarate signaling pathway.
实验应用 : WB
种属反应 : Human
Ishiyama A et al. Neurol Genet 2017; 3 (5):e184 IBA57 mutations abrogate iron-sulfur cluster assembly leading to cavitating leukoencephalopathy.
实验应用 : WB
种属反应 : Human
Lee H et al. J Biol Chem 2017; (Epub) The Short Variant of the Mitochondrial Dynamin OPA1 Maintains Mitochondrial Energetics and Cristae Structure.
实验应用 : WB
种属反应 : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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