产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human SERCA2 ATPase. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 2 , ATP2B , DAR , DD , SERCA2
细胞定位
Endoplasmic reticulum membrane
功能与背景
This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol into the sarcoplasmic reticulum lumen, and is involved in regulation of the contraction/relaxation cycle. Mutations in this gene cause Darier-White disease, also known as keratosis follicularis, an autosomal dominant skin disorder characterized by loss of adhesion between epidermal cells and abnormal keratinization. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2008]
数据库
研究领域
產品图片
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GTX638555 WB Image
Non-transfected (–) and transfected (+) A549 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SERCA2 ATPase antibody [HL2352] (GTX638555) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638555 IHC-P Image
SERCA2 ATPase antibody [HL2352] detects SERCA2 ATPase protein by immunohistochemical analysis.Sample: Paraffin-embedded cat brain.SERCA2 ATPase stained by SERCA2 ATPase antibody [HL2352] (GTX638555) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638555 IHC-P Image
SERCA2 ATPase antibody [HL2352] detects SERCA2 ATPase protein by immunohistochemical analysis.Sample: Paraffin-embedded dog tissues.SERCA2 ATPase stained by SERCA2 ATPase antibody [HL2352] (GTX638555) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638555 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SERCA2 ATPase antibody [HL2352] (GTX638555) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX638555 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SERCA2 ATPase antibody [HL2352] (GTX638555) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX638555 IHC-P Image
SERCA2 ATPase antibody [HL2352] detects SERCA2 ATPase protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse liver.SERCA2 ATPase stained by SERCA2 ATPase antibody [HL2352] (GTX638555) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638555 IHC-P Image
SERCA2 ATPase antibody [HL2352] detects SERCA2 ATPase protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human hepatocellular carcinoma.SERCA2 ATPase stained by SERCA2 ATPase antibody [HL2352] (GTX638555) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638555 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SERCA2 ATPase antibody [HL2352] (GTX638555) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638555 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SERCA2 ATPase antibody [HL2352] (GTX638555) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX638555 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SERCA2 ATPase antibody [HL2352] (GTX638555) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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