产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:1000-1:10000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
BCL-1
预测种属反应
Rat, Bovine, Chicken, Xenopus tropicalis(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1.5 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the N-terminus region of human SERCA3. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_11166236
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
ATPase sarcoplasmic/endoplasmic reticulum Ca2+ transporting 3 , SERCA3
细胞定位
Nucleus membrane , Endoplasmic reticulum membrane , Sarcoplasmic reticulum membrane
功能与背景
This gene encodes one of the SERCA Ca(2+)-ATPases, which are intracellular pumps located in the sarcoplasmic or endoplasmic reticula of muscle cells. This enzyme catalyzes the hydrolysis of ATP coupled with the translocation of calcium from the cytosol to the sarcoplasmic reticulum lumen, and is involved in calcium sequestration associated with muscular excitation and contraction. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq]
数据库
研究领域
產品图片
![Anti-SERCA3 antibody [N1N2], N-term used in Western Blot (WB). GTX102381](/templates/default/js/lightbox/images/loading.gif "Anti-SERCA3 antibody [N1N2], N-term used in Western Blot (WB). GTX102381")
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GTX102381 WB Image
Sample (30 ug of whole cell lysate)
A: BCL-1
5% SDS PAGE
GTX102381 diluted at 1:5000
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GTX102381 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using SERCA3(GTX102381) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX102381 WB Image
Jurkat whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SERCA3 antibody [N1N2], N-term (GTX102381) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献引用
产品评论
目前没有使用 SERCA3 antibody [N1N2], N-term (GTX102381) 的产品评论被收录。快发表第一篇产品评论吧！
![Anti-SERCA3 antibody [N1N2], N-term used in Western Blot (WB). GTX102381](/upload/website/prouct_img/small/GTX102381/GTX102381_40758_WB_M_w_23060100_690.webp "Anti-SERCA3 antibody [N1N2], N-term used in Western Blot (WB). GTX102381")
![Anti-SERCA3 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102381](/upload/website/prouct_img/small/GTX102381/GTX102381_40758_IHC_w_23060100_134.webp "Anti-SERCA3 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102381")
![Anti-SERCA3 antibody [N1N2], N-term used in Western Blot (WB). GTX102381](/upload/website/prouct_img/small/GTX102381/GTX102381_44125_20210723_WB_Fraction_w_23060100_696.webp "Anti-SERCA3 antibody [N1N2], N-term used in Western Blot (WB). GTX102381")
![Anti-SERCA3 antibody [N1N2], N-term used in Western Blot (WB). GTX102381](/upload/website/prouct_img/normal/GTX102381/GTX102381_40758_WB_M_w_23060100_690.webp "Anti-SERCA3 antibody [N1N2], N-term used in Western Blot (WB). GTX102381")
![Anti-SERCA3 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102381](/upload/website/prouct_img/normal/GTX102381/GTX102381_40758_IHC_w_23060100_134.webp "Anti-SERCA3 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX102381")
![Anti-SERCA3 antibody [N1N2], N-term used in Western Blot (WB). GTX102381](/upload/website/prouct_img/normal/GTX102381/GTX102381_44125_20210723_WB_Fraction_w_23060100_696.webp "Anti-SERCA3 antibody [N1N2], N-term used in Western Blot (WB). GTX102381")
