产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:1000-1:10000 |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
预测种属反应
Bovine(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human SETDB1 The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
SET domain bifurcated histone lysine methyltransferase 1 , ESET , H3-K9-HMTase4 , KG1T , KMT1E , TDRD21
细胞定位
Nucleus,Cytoplasm
功能与背景
This gene encodes a histone methyltransferase which regulates histone methylation, gene silencing, and transcriptional repression. This gene has been identified as a target for treatment in Huntington Disease, given that gene silencing and transcription dysfunction likely play a role in the disease pathogenesis. Alternatively spliced transcript variants of this gene have been described.[provided by RefSeq, Jun 2011]
数据库
研究领域
產品图片
![Anti-SETDB1 antibody [HL1645] used in Western Blot (WB). GTX637226](/templates/default/js/lightbox/images/loading.gif "Anti-SETDB1 antibody [HL1645] used in Western Blot (WB). GTX637226")
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GTX637226 WB Image
HeLa whole cell and nuclear extract (30 μg) were separated by 5% SDS-PAGE, and the membranes were blotted with SETDB1 antibody [HL1645] (GTX637226) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX637226 WB Image
Various tissue extracts (50 Âμg) were separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [HL1645] (GTX637226) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637226 IHC-P Image
SETDB1 antibody [HL1645] detects SETDB1 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.SETDB1 stained by SETDB1 antibody [HL1645] (GTX637226) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637226 WB Image
HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SETDB1 antibody [HL1645] (GTX637226) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637226 ICC/IF Image
SETDB1 antibody [HL1645] detects SETDB1 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SETDB1 stained by SETDB1 antibody [HL1645] (GTX637226) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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文献引用
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产品评论
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![Anti-SETDB1 antibody [HL1645] used in Western Blot (WB). GTX637226](/upload/website/prouct_img/small/GTX637226/GTX637226_44830_20230630_WB_Competitor_watermark_23062718_150.webp "Anti-SETDB1 antibody [HL1645] used in Western Blot (WB). GTX637226")
![Anti-SETDB1 antibody [HL1645] used in Western Blot (WB). GTX637226](/upload/website/prouct_img/small/GTX637226/GTX637226_T-44746_20220805_WB_M_R_22080923_527.webp "Anti-SETDB1 antibody [HL1645] used in Western Blot (WB). GTX637226")
![Anti-SETDB1 antibody [HL1645] used in IHC (Paraffin sections) (IHC-P). GTX637226](/upload/website/prouct_img/small/GTX637226/GTX637226_T-44746_20220819_IHC-P_M_22083119_273.webp "Anti-SETDB1 antibody [HL1645] used in IHC (Paraffin sections) (IHC-P). GTX637226")
![Anti-SETDB1 antibody [HL1645] used in Western Blot (WB). GTX637226](/upload/website/prouct_img/small/GTX637226/GTX637226_44830_20221014_WB_Fraction_22101718_496.webp "Anti-SETDB1 antibody [HL1645] used in Western Blot (WB). GTX637226")
![Anti-SETDB1 antibody [HL1645] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637226](/upload/website/prouct_img/small/GTX637226/GTX637226_T-44746_20220902_ICC_IF_22101718_996.webp "Anti-SETDB1 antibody [HL1645] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637226")
![Anti-SETDB1 antibody [HL1645] used in Western Blot (WB). GTX637226](/upload/website/prouct_img/normal/GTX637226/GTX637226_44830_20230630_WB_Competitor_watermark_23062718_150.webp "Anti-SETDB1 antibody [HL1645] used in Western Blot (WB). GTX637226")
![Anti-SETDB1 antibody [HL1645] used in Western Blot (WB). GTX637226](/upload/website/prouct_img/normal/GTX637226/GTX637226_T-44746_20220805_WB_M_R_22080923_527.webp "Anti-SETDB1 antibody [HL1645] used in Western Blot (WB). GTX637226")
![Anti-SETDB1 antibody [HL1645] used in IHC (Paraffin sections) (IHC-P). GTX637226](/upload/website/prouct_img/normal/GTX637226/GTX637226_T-44746_20220819_IHC-P_M_22083119_273.webp "Anti-SETDB1 antibody [HL1645] used in IHC (Paraffin sections) (IHC-P). GTX637226")
![Anti-SETDB1 antibody [HL1645] used in Western Blot (WB). GTX637226](/upload/website/prouct_img/normal/GTX637226/GTX637226_44830_20221014_WB_Fraction_22101718_496.webp "Anti-SETDB1 antibody [HL1645] used in Western Blot (WB). GTX637226")
![Anti-SETDB1 antibody [HL1645] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637226](/upload/website/prouct_img/normal/GTX637226/GTX637226_T-44746_20220902_ICC_IF_22101718_996.webp "Anti-SETDB1 antibody [HL1645] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637226")
