Untreated (–) and treated (+) A431 whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with SHP2 (phospho Tyr542) antibody [HL3334] (GTX641099) diluted at 1:100000 and SHP2 antibody (GTX101062) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SHP2 (phospho Tyr542) antibody [HL3334] (GTX641099) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.