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SIGMAR1抗体 [HL2230]

Recombinant antibody KO/KD
Anti-SIGMAR1 antibody [HL2230] used in Western Blot (WB). GTX638275
Anti-SIGMAR1 antibody [HL2230] used in Western Blot (WB). GTX638275
Anti-SIGMAR1 antibody [HL2230] used in IHC (Paraffin sections) (IHC-P). GTX638275
Anti-SIGMAR1 antibody [HL2230] used in Western Blot (WB). GTX638275
Anti-SIGMAR1 antibody [HL2230] used in Western Blot (WB). GTX638275

目录号 GTX638275

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL2230

同种型

 IgG

实验应用

 WB, IHC-P

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
IHC-P Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

25 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant fragment of human SIGMAR1.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

sigma non-opioid intracellular receptor 1 , ALS16 , DSMA2 , OPRS1 , SIG-1R , SR-BP , SR-BP1 , SRBP , hSigmaR1 , sigma1R

细胞定位

Nucleus inner membrane , Nucleus outer membrane , Cytoplasmic vesicle , Endoplasmic reticulum membrane , Membrane , Cell junction , Cell membrane , Cell projection, growth cone , Postsynaptic density

功能与背景

This gene encodes a receptor protein that interacts with a variety of psychotomimetic drugs, including cocaine and amphetamines. The receptor is believed to play an important role in the cellular functions of various tissues associated with the endocrine, immune, and nervous systems. As indicated by its previous name, opioid receptor sigma 1 (OPRS1), the product of this gene was erroneously thought to function as an opioid receptor; it is now thought to be a non-opioid receptor. Mutations in this gene has been associated with juvenile amyotrophic lateral sclerosis 16. Alternative splicing of this gene results in transcript variants encoding distinct isoforms. [provided by RefSeq, Aug 2013]

数据库

研究领域

產品图片

Anti-SIGMAR1 antibody [HL2230] used in Western Blot (WB). GTX638275

GTX638275 WB Image

KO/KD

Wild-type (WT) and SIGMAR1 knockout (KO) 293T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SIGMAR1 antibody [HL2230] (GTX638275) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SIGMAR1 antibody [HL2230] used in Western Blot (WB). GTX638275

GTX638275 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SIGMAR1 antibody [HL2230] (GTX638275) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SIGMAR1 antibody [HL2230] used in IHC (Paraffin sections) (IHC-P). GTX638275

GTX638275 IHC-P Image

SIGMAR1 antibody [HL2230] detects SIGMAR1 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
SIGMAR1 stained by SIGMAR1 antibody [HL2230] (GTX638275) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SIGMAR1 antibody [HL2230] used in Western Blot (WB). GTX638275

GTX638275 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SIGMAR1 antibody [HL2230] (GTX638275) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SIGMAR1 antibody [HL2230] used in Western Blot (WB). GTX638275

GTX638275 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SIGMAR1 antibody [HL2230] (GTX638275) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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产品包装 报价 (¥)
¥ 4000
¥ 1700
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