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SIN3A抗体

Anti-SIN3A antibody used in Western Blot (WB). GTX129156
Anti-SIN3A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129156
Anti-SIN3A antibody used in IHC (Paraffin sections) (IHC-P). GTX129156
Anti-SIN3A antibody used in Western Blot (WB). GTX129156
Anti-SIN3A antibody used in IHC (Paraffin sections) (IHC-P). GTX129156
Anti-SIN3A antibody used in Immunoprecipitation (IP). GTX129156
Anti-SIN3A antibody used in IHC (Paraffin sections) (IHC-P). GTX129156
Anti-SIN3A antibody used in Western Blot (WB). GTX129156
Anti-SIN3A antibody used in ChIP assay (ChIP assay). GTX129156

目录号 GTX129156

宿主

Rabbit

克隆

Polyclonal

同种型

IgG

实验应用

WB, ICC/IF, IHC-P, IP, ChIP assay

种属反应

Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
ChIP assay Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

145 kDa. ( Note )

阳性对照

293T , A431 , HeLa , HepG2 , mouse brain , rat brain

产品说明

Knockdown/Knockout validation was supported by references (PMID:28805661)

预测种属反应

Chicken, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.38 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the N-terminus region of human SIN3A. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2885913

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

SIN3 transcription regulator family member A , WITKOS

细胞定位

Nucleus , nucleolus

功能与背景

The protein encoded by this gene is a transcriptional regulatory protein. It contains paired amphipathic helix (PAH) domains, which are important for protein-protein interactions and may mediate repression by the Mad-Max complex. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-SIN3A antibody used in Western Blot (WB). GTX129156

GTX129156 WB Image

HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SIN3A antibody (GTX129156) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SIN3A antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129156

GTX129156 ICC/IF Image

SIN3A antibody detects SIN3A protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SIN3A stained by SIN3A antibody (GTX129156) diluted at 1:1000.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar= 10 μm.

Anti-SIN3A antibody used in IHC (Paraffin sections) (IHC-P). GTX129156

GTX129156 IHC-P Image

SIN3A antibody detects SIN3A protein at nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
SIN3A antibody (GTX129156) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SIN3A antibody used in Western Blot (WB). GTX129156

GTX129156 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SIN3A antibody (GTX129156) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SIN3A antibody used in IHC (Paraffin sections) (IHC-P). GTX129156

GTX129156 IHC-P Image

SIN3A antibody detects SIN3A protein at nucleus on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
SIN3A antibody (GTX129156) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SIN3A antibody used in Immunoprecipitation (IP). GTX129156

GTX129156 IP Image

SIN3A antibody immunoprecipitates SIN3A protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 μg whole cell lysate/extract of SIN3A protein expressing 293T cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of SIN3A by 3 μg of SIN3A antibody (GTX129156) 5% SDS-PAGE The immunoprecipitated SIN3A protein was detected by SIN3A antibody (GTX129156) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-SIN3A antibody used in IHC (Paraffin sections) (IHC-P). GTX129156

GTX129156 IHC-P Image

SIN3A antibody detects SIN3A protein at nucleus on rat fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat fore brain.
SIN3A antibody (GTX129156) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SIN3A antibody used in Western Blot (WB). GTX129156

GTX129156 WB Image

SIN3A antibody detects SIN3A protein by western blot analysis.
A 293T whole cell lysate/extract
B A431 whole cell lysate/extract
C HeLa whole cell lysate/extract
D HepG2 whole cell lysate/extract
5% SDS-PAGE
SIN3A antibody (GTX129156) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SIN3A antibody used in ChIP assay (ChIP assay). GTX129156

GTX129156 ChIP assay Image

Cross-linked ChIP was performed with MCF-7 chromatin extract treated with B-estradiol (10 nM for 45 min) and 5 μg of either control rabbit IgG or anti-SIN3A antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH or MyoD.

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PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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