产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
1:100-1:500 |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
293T , A431 , HeLa , HepG2 , mouse brain , rat brain
产品说明
Knockdown/Knockout validation was supported by references (PMID:28805661)
预测种属反应
Chicken, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1.38 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the N-terminus region of human SIN3A. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2885913
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
SIN3 transcription regulator family member A , WITKOS
细胞定位
Nucleus , nucleolus
功能与背景
The protein encoded by this gene is a transcriptional regulatory protein. It contains paired amphipathic helix (PAH) domains, which are important for protein-protein interactions and may mediate repression by the Mad-Max complex. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX129156 WB Image
HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SIN3A antibody (GTX129156) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX129156 ICC/IF Image
SIN3A antibody detects SIN3A protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SIN3A stained by SIN3A antibody (GTX129156) diluted at 1:1000.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar= 10 μm.
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GTX129156 IHC-P Image
SIN3A antibody detects SIN3A protein at nucleus on mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon. SIN3A antibody (GTX129156) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX129156 WB Image
Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with SIN3A antibody (GTX129156) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX129156 IHC-P Image
SIN3A antibody detects SIN3A protein at nucleus on mouse prostate by immunohistochemical analysis. Sample: Paraffin-embedded mouse prostate. SIN3A antibody (GTX129156) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX129156 IP Image
SIN3A antibody immunoprecipitates SIN3A protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 μg whole cell lysate/extract of SIN3A protein expressing 293T cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of SIN3A by 3 μg of SIN3A antibody (GTX129156) 5% SDS-PAGE The immunoprecipitated SIN3A protein was detected by SIN3A antibody (GTX129156) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
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GTX129156 IHC-P Image
SIN3A antibody detects SIN3A protein at nucleus on rat fore brain by immunohistochemical analysis. Sample: Paraffin-embedded rat fore brain. SIN3A antibody (GTX129156) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX129156 WB Image
SIN3A antibody detects SIN3A protein by western blot analysis. A 293T whole cell lysate/extract B A431 whole cell lysate/extract C HeLa whole cell lysate/extract D HepG2 whole cell lysate/extract 5% SDS-PAGE SIN3A antibody (GTX129156) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX129156 ChIP assay Image
Cross-linked ChIP was performed with MCF-7 chromatin extract treated with B-estradiol (10 nM for 45 min) and 5 μg of either control rabbit IgG or anti-SIN3A antibody. The precipitated DNA was detected by PCR with primer set targeting to GAPDH or MyoD.
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