产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
1:100-1:1000 |
1:500-1:1000 |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
HeLa , SMAD2-transfected 293T
产品说明
This antibody may recognize other Smad family members.
预测种属反应
Rat, Zebrafish, Bovine, Chicken, Pig, Xenopus tropicalis, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
0.1M Tris, 0.1M Glycine, 10% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human SMAD2. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2038000
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
SMAD family member 2 , JV18 , JV18-1 , MADH2 , MADR2 , hMAD-2 , hSMAD2
细胞定位
Cytoplasm , Nucleus
功能与背景
The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the same protein have been observed. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX111131 IP Image
SMAD2 antibody immunoprecipitates SMAD2 protein in IP experiments. IP Sample: 1000 μg HeLa whole cell lysate/extract A. 50 μg HeLa whole cell lysate/extract B. Control with 2 μg of preimmune rabbit IgG C. Immunoprecipitation of SMAD2protein by 2 μg of SMAD2antibody (GTX111131) 10% SDS-PAGE The immunoprecipitated SMAD2protein was detected by SMAD2antibody (GTX111131) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX111131 ChIP assay Image
SMAD2 antibody immunoprecipitates SMAD2 protein-DNA in ChIP experiments. ChIP Sample: HeLa whole cell lysate/extract A. 5 μg preimmune rabbit IgG B. 5 μg of SMAD2 antibody (GTX111131) The precipitated DNA was detected by PCR with primer set targeting to E-cadherin promoter.
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GTX111131 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD2 antibody (GTX111131) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX111131 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using SMAD2(GTX111131) antibody at 1:200 dilution.
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GTX111131 WB Image
Sample (30 μg of whole cell lysate) A: HeLa 10% SDS PAGE GTX111131 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX111131 WB Image
The data was published in the journal Cell Death Dis in 2015. PMID: 25766327
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GTX111131 WB Image
The data was published in the journal Cell Death Dis in 2015. PMID: 25766327
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GTX111131 WB Image
The data was published in the journal Cell Death Dis in 2015. PMID: 25766327
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文献引用
产品评论
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