GeneTex中国官方网站
  • China

SMAD3 (phospho Ser423/Ser425)抗体 [GT1207]

Recombinant antibody Orthogonal
Anti-SMAD3 (phospho Ser423/Ser425) antibody [GT1207] used in Western Blot (WB). GTX00969
Anti-SMAD3 (phospho Ser423/Ser425) antibody [GT1207] used in IHC (Paraffin sections) (IHC-P). GTX00969
Anti-SMAD3 (phospho Ser423/Ser425) antibody [GT1207] used in IHC (Paraffin sections) (IHC-P). GTX00969
Anti-SMAD3 (phospho Ser423/Ser425) antibody [GT1207] used in Western Blot (WB). GTX00969
Anti-SMAD3 (phospho Ser423/Ser425) antibody [GT1207] used in IHC (Paraffin sections) (IHC-P). GTX00969

目录号 GTX00969

文献引用 4
1

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 GT1207

同种型

 IgG

实验应用

 WB, IHC-P

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥5600)
查看所有 SMAD3 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

48 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS, 50% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

Batch dependent (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

A synthesized peptide derived from human Phospho-Smad3 (S423 + S425).

纯化方式


Purified by affinity chromatography

偶联

Unconjugated

RRID

AB_2885209

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

SMAD family member 3 , HSPC193 , HsT17436 , JV152 , LDS1C , LDS3 , MADH3 , SMAD3

细胞定位

Cytoplasm,Nucleus

功能与背景

The protein encoded by this gene belongs to the SMAD, a family of proteins similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein functions as a transcriptional modulator activated by transforming growth factor-beta and is thought to play a role in the regulation of carcinogenesis. [provided by RefSeq, Apr 2009]

数据库

研究领域

產品图片

Anti-SMAD3 (phospho Ser423/Ser425) antibody [GT1207] used in Western Blot (WB). GTX00969

GTX00969 WB Image

Orthogonal

Untreated (–) and treated (+) A549 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD3 (phospho Ser423/Ser425) antibody [GT1207] (GTX00969) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SMAD3 (phospho Ser423/Ser425) antibody [GT1207] used in IHC (Paraffin sections) (IHC-P). GTX00969

GTX00969 IHC-P Image

IHC-P analysis of rat brain tissue section using GTX00969 SMAD3 (phospho Ser423/Ser425) antibody [GT1207].
Dilution : 1:100

Anti-SMAD3 (phospho Ser423/Ser425) antibody [GT1207] used in IHC (Paraffin sections) (IHC-P). GTX00969

GTX00969 IHC-P Image

IHC-P analysis of mouse testis tissue section using GTX00969 SMAD3 (phospho Ser423/Ser425) antibody [GT1207].
Dilution : 1:100

Anti-SMAD3 (phospho Ser423/Ser425) antibody [GT1207] used in Western Blot (WB). GTX00969

GTX00969 WB Image

Orthogonal

WB analysis of C6 cell extracts with/without PMA/TPA (200 nM, 37ºC, 10 min) treatment using GTX00969 SMAD3 (phospho Ser423/Ser425) antibody [GT1207].
Dilution : 1:1000
Loading : 25μg

Anti-SMAD3 (phospho Ser423/Ser425) antibody [GT1207] used in IHC (Paraffin sections) (IHC-P). GTX00969

GTX00969 IHC-P Image

IHC-P analysis of human esophageal tissue section using GTX00969 SMAD3 (phospho Ser423/Ser425) antibody [GT1207].
Dilution : 1:100

文献引用

Application Reference

Luo D et al. J Cell Mol Med 2023; 27 (3) : 456-469 Pirfenidone suppressed triple-negative breast cancer metastasis by inhibiting the activity of the TGF-β/SMAD pathway.
实验应用 : WB
种属反应 : Human
Wenbiao Chen et al. Cell Commun Signal 2022; 20 (1) : 28 Ameliorating role of microRNA-378 carried by umbilical cord mesenchymal stem cells-released extracellular vesicles in mesangial proliferative glomerulonephritis.
实验应用 : WB
种属反应 : Rat
Chen HY et al. Antioxidants (Basel) 2021; 10 (1) Characterization of TGF-β by Induced Oxidative Stress in Human Trabecular Meshwork Cells.
实验应用 : WB
种属反应 : Human
Mar?n-Ramos NI et al. Circ Res 2020; 127 (9) : e210-e231 miR-18a Inhibits BMP4 and HIF-1α Normalizing Brain Arteriovenous Malformations.
实验应用 : IHC-P
种属反应 : Human
我要回报文献

产品评论

SMAD3 (phospho Ser423/Ser425) antibody [GT1207] 目录号 GTX00969

Rating
( Average 5 based on 1 users reviews)
实验应用
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-SMAD3 (phospho Ser423/Ser425) antibody [GT1207] used in Western Blot (WB). GTX00969
Date : Anonymous submitted on 19-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : PC-3 cells
Amount used : 30 μg
Blocking : 5% non-fat milk in TBST, RT°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
条带说明 :
Lane1: 24 h after irradiation with 0Gy
Lane2: 24 h after irradiation with 2Gy
Lane3: 24 h after irradiation with 4Gy
Lane4: 24 h after irradiation with 6Gy
Lane5: 24 h after irradiation with 8Gy
Lane6: 24 h after irradiation with 12Gy
我要评论产品
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
产品包装 报价 (¥)
¥ 5600
联系经销商
欣博盛生物科技有限公司
欣博盛生物科技有限公司
(全国热线) 4006-800-892 / (深圳) 0755-26755892 / (北京) 010-88594029 / (上海) 021-34613729 / (广州) 18024516375 / (香港) +852-69410778
http://www.neobioscience.com
genetex@neobioscience.com