产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2 , 293T , A431 , HeLa , HepG2 , DDDDK-tagged SMAD4-transfected 293T
预测种属反应
Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.58 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the C-terminus region of human SMAD4. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_1951939
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
SMAD family member 4 , DPC4 , JIP , MADH4 , MYHRS
细胞定位
Cytoplasm , Nucleus
功能与背景
This gene encodes a member of the Smad family of signal transduction proteins. Smad proteins are phosphorylated and activated by transmembrane serine-threonine receptor kinases in response to TGF-beta signaling. The product of this gene forms homomeric complexes and heteromeric complexes with other activated Smad proteins, which then accumulate in the nucleus and regulate the transcription of target genes. This protein binds to DNA and recognizes an 8-bp palindromic sequence (GTCTAGAC) called the Smad-binding element (SBE). The Smad proteins are subject to complex regulation by post-translational modifications. Mutations or deletions in this gene have been shown to result in pancreatic cancer, juvenile polyposis syndrome, and hereditary hemorrhagic telangiectasia syndrome. [provided by RefSeq]
数据库
研究领域
產品图片
|
GTX112980 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with SMAD4 antibody (GTX112980) diluted at 1:1000 and competitor's antibody (sc-7966) diluted at 1:100. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
|
|
|
GTX112980 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD4 antibody (GTX112980) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX112980 IHC-P Image
SMAD4 antibody detects SMAD4 protein at cytoplasm and nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
SMAD4 antibody (GTX112980) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX112980 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human ulcerative colitis tissue using SMAD4 antibody (GTX112980)
|
|
|
GTX112980 ICC/IF Image
SMAD4 antibody detects SMAD4 protein a tcytoplasm and nucleus by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SMAD4 protein stained by SMAD4 antibody (GTX112980) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.
|
|
|
GTX112980 ICC/IF Image
SMAD4 antibody detects SMAD4 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SMAD4 protein stained by SMAD4 antibody (GTX112980) diluted at 1:500.
Blue: Hoechst 33342 staining.
|
|
|
GTX112980 IHC-P Image
SMAD4 antibody detects SMAD4 protein at cytoplasm and nucleus in mouse duodenum by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
SMAD4 antibody (GTX112980) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX112980 IHC-P Image
SMAD4 antibody detects SMAD4 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse testis.SMAD4 stained by SMAD4 antibody (GTX112980) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX112980 IHC-P Image
SMAD4 antibody detects SMAD4 protein at cytoplasm and nucleus in rat kidney by immunohistochemical analysis.
Sample: Paraffin-embedded rat kidney.
SMAD4 antibody (GTX112980) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX112980 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMAD4 antibody (GTX112980) diluted at 1:1000.
|
|
|
GTX112980 WB Image
SMAD4 antibody detects SMAD4 protein by western blot analysis. Various whole cell extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with SMAD4 antibody (GTX112980) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
|
GTX112980 IHC-P Image
SMAD4 antibody detects SMAD4 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.SMAD4 stained by SMAD4 antibody (GTX112980) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
文献引用
产品评论
目前没有使用 SMAD4 antibody (GTX112980) 的产品评论被收录。快发表第一篇产品评论吧！
