产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fragment of human SMYD3.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
SET and MYND domain containing 3 , KMT3E , ZMYND1 , ZNFN3A1 , bA74P14.1
细胞定位
Cytoplasm,Nucleus
功能与背景
This gene encodes a histone methyltransferase which functions in RNA polymerase II complexes by an interaction with a specific RNA helicase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
数据库
研究领域
產品图片
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GTX638782 IHC-P Image
SMYD3 antibody [HL2464] detects SMYD3 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.SMYD3 stained by SMYD3 antibody [HL2464] (GTX638782) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638782 IHC-P Image
SMYD3 antibody [HL2464] detects SMYD3 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse colon.SMYD3 stained by SMYD3 antibody [HL2464] (GTX638782) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638782 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody [HL2464] (GTX638782) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX638782 WB Image
Whole zebrafish extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody [HL2464] (GTX638782) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.
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GTX638782 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody [HL2464] (GTX638782) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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文献引用
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产品评论
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