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SNAI1抗体

Comparison Orthogonal Overexpression
Anti-SNAI1 antibody used in Western Blot (WB). GTX100754
Anti-SNAI1 antibody used in Western Blot (WB). GTX100754
Anti-SNAI1 antibody used in Western Blot (WB). GTX100754
Anti-SNAI1 antibody used in Western Blot (WB). GTX100754
Anti-SNAI1 antibody used in Western Blot (WB). GTX100754
Anti-SNAI1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100754
Anti-SNAI1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100754
Anti-SNAI1 antibody used in Western Blot (WB). GTX100754
Anti-SNAI1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100754

目录号 GTX100754

文献引用 25
1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

29 kDa. ( Note )

阳性对照

U87-MG , SK-N-SH , IMR32 , SK-N-AS , HeLa (starvation for 16 hr 20 ng/ml TGF-beta and 300 ng/ml EGF treatment for 30 min) , SNAI1-transfected 293T

预测种属反应

Bovine(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.54 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human SNAI1. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_10618674

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

snail family transcriptional repressor 1 , SLUGH2 , SNA , SNAH , SNAIL , SNAIL1 , dJ710H13.1

细胞定位

Nucleus

功能与背景

The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-SNAI1 antibody used in Western Blot (WB). GTX100754

GTX100754 WB Image

Comparison Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (50 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with SNAI1 antibody (GTX100754) diluted at 1:1000 and competitor's antibody (#3879) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-SNAI1 antibody used in Western Blot (WB). GTX100754

GTX100754 WB Image

Orthogonal

Untreated (–) and treated (+) Hela whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody (GTX100754) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SNAI1 antibody used in Western Blot (WB). GTX100754

GTX100754 WB Image

SNAI1 antibody detects SNAI1 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody (GTX100754) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SNAI1 antibody used in Western Blot (WB). GTX100754

GTX100754 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody (GTX100754) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SNAI1 antibody used in Western Blot (WB). GTX100754

GTX100754 WB Image

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody (GTX100754) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SNAI1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100754

GTX100754 ICC/IF Image

Orthogonal

SNAI1 antibody detects SNAI1 protein by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SNAI1 stained by SNAI1 antibody (GTX100754) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SNAI1 antibody used in IHC (Paraffin sections) (IHC-P). GTX100754

GTX100754 IHC-P Image

SNAI1 antibody detects SNAI1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
SNAI1 stained by SNAI1 antibody (GTX100754) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SNAI1 antibody used in Western Blot (WB). GTX100754

GTX100754 WB Image

Orthogonal

The data was published in the journal Sci Rep in 2017. PMID: 28134285

Anti-SNAI1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100754

GTX100754 ICC/IF Image

The data was published in the journal Oncotarget in 2017.PMID: 27494900

文献引用

Application Reference

Wang CW et al. Front Immunol 2023; 14 : 1225348 Arsenic exposure and lung fibrotic changes-evidence from a longitudinal cohort study and experimental models.
Chiou HC et al. Antioxidants (Basel) 2023; 12 (2) Copper Exposure Induces Epithelial-Mesenchymal Transition-Related Fibrotic Change via Autophagy and Increase Risk of Lung Fibrosis in Human.
实验应用 : WB
种属反应 : Human
Kano H et al. Cancer Sci 2023; 114 (4) : 1479-1490 Suppression of androgen receptor signaling induces prostate cancer migration via activation of the CCL20-CCR6 axis.
实验应用 : IHC-P
种属反应 : Human
Yin-Quan Chen et al. Front Cell Dev Biol 2022; 10 : 809738 Snail Augments Nuclear Deformability to Promote Lymph Node Metastasis of Head and Neck Squamous Cell Carcinoma.
实验应用 : ICC/IF
种属反应 : Human
Li Xiao et al. Int J Mol Sci 2021; 22 (19) LEF1 Enhances the Progression of Colonic Adenocarcinoma via Remodeling the Cell Motility Associated Structures.
实验应用 : WB
种属反应 : Human
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产品评论

SNAI1 antibody 目录号 GTX100754

Rating
( Average 4 based on 1 users reviews)
实验应用
Western Blot(WB)
( Average 4 based on 1 users reviews)
Anti-SNAI1 antibody used in Western Blot (WB). GTX100754
Date : Anonymous submitted on 03-Oct-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : TW2.6 cells
Amount used : 15μg
Blocking : 5% milk in PBST, 25°C, 1Hr
Primary Antibody : 1:3000, 25°C, 1Hr
我要评论产品
Application Reference
Wang CW et al. Front Immunol 2023; 14:1225348 Arsenic exposure and lung fibrotic changes-evidence from a longitudinal cohort study and experimental models.
Chiou HC et al. Antioxidants (Basel) 2023; 12 (2) Copper Exposure Induces Epithelial-Mesenchymal Transition-Related Fibrotic Change via Autophagy and Increase Risk of Lung Fibrosis in Human.
实验应用 : WB
种属反应 : Human
Kano H et al. Cancer Sci 2023; 114 (4):1479-1490 Suppression of androgen receptor signaling induces prostate cancer migration via activation of the CCL20-CCR6 axis.
实验应用 : IHC-P
种属反应 : Human
Yin-Quan Chen et al. Front Cell Dev Biol 2022; 10:809738 Snail Augments Nuclear Deformability to Promote Lymph Node Metastasis of Head and Neck Squamous Cell Carcinoma.
实验应用 : ICC/IF
种属反应 : Human
Li Xiao et al. Int J Mol Sci 2021; 22 (19) LEF1 Enhances the Progression of Colonic Adenocarcinoma via Remodeling the Cell Motility Associated Structures.
实验应用 : WB
种属反应 : Human
Brzozowa-Zasada M et al. Prz Gastroenterol 2021; 16 (4):339-345 Immunohistochemical expression of Snail1 protein in colorectal adenocarcinoma samples and its prognostic activity in Caucasian patients.
实验应用 : IHC-P
种属反应 : Human
Chung HH et al. Int J Mol Sci 2020; 21 (14) NKX6.1 Represses Tumorigenesis, Metastasis, and Chemoresistance in Colorectal Cancer.
Chang YX et al. Cancers (Basel) 2020; 12 (12) Chaperonin-Containing TCP-1 Promotes Cancer Chemoresistance and Metastasis through the AKT-GSK3β-β-Catenin and XIAP-Survivin Pathways.
实验应用 : WB
种属反应 : Human
Xiao L et al. Life Sci 2020; 253:117740 Roles of N-terminal Annexin A2 phosphorylation sites and miR-206 in colonic adenocarcinoma.
实验应用 : WB
种属反应 : Human
Li JM et al. Ther Adv Med Oncol 2018; 10:1.76E+15 Upregulation of LGALS1 is associated with oral cancer metastasis.
实验应用 : WB
种属反应 : Human
Chang CC et al. Sci Rep 2018; 8 (1):9786 The role of sentrin-specific protease 2 substrate recognition in TGF-β-induced tumorigenesis.
实验应用 : WB
种属反应 : Human
Chen CY et al. Int J Mol Sci 2018; 19 (2) Corylin Suppresses Hepatocellular Carcinoma Progression via the Inhibition of Epithelial-Mesenchymal Transition, Mediated by Long Noncoding RNA GAS5.
实验应用 : WB
种属反应 : Human
Roth B et al. Oncotarget 2017; 8 (21):34205-34222 Employing an orthotopic model to study the role of epithelial-mesenchymal transition in bladder cancer metastasis.
实验应用 : ICC/IF
种属反应 : Mouse
Chou HC et al. Toxicological Sciences 2017; (Epub) L-carnitine via PPARγ- and Sirt1-dependent mechanisms attenuates epithelial–mesenchymal transition and renal fibrosis caused by perfluorooctanesulfonate.
实验应用 : WB
Hsu LS et al. Int J Med Sci 2017; 14 (6):530-535 KLF6 inhibited oral cancer migration and invasion via downregulation of mesenchymal markers and inhibition of MMP-9 activities.
实验应用 : WB
种属反应 : Human
Wang LT et al. Oncogene 2017; (Epub) Transcription factor SPZ1 promotes TWIST-mediated epithelial-mesenchymal transition and oncogenesis in human liver cancer.
实验应用 : WB
种属反应 : Human
Liu YH et al. Sci Rep 2017; 7:41437 Aqueous extract of Polygonum bistorta modulates proteostasis by ROS-induced ER stress in human hepatoma cells.
实验应用 : WB
种属反应 : Human
Li HJ et al. Oncogene 2016; 35 (17):2266-78 NKX6.1 functions as a metastatic suppressor through epigenetic regulation of the epithelial-mesenchymal transition.
实验应用 : WB
种属反应 : Human
Chiu WC et al. PLoS One 2016; 11 (11):e0166453 The Synthetic β-Nitrostyrene Derivative CYT-Rx20 Inhibits Esophageal Tumor Growth and Metastasis via PI3K/AKT and STAT3 Pathways.
实验应用 : WB
种属反应 : Human
Yang HL et al. Biochim Biophys Acta 2016; 1864 (1):151-168 Lucidone Promotes the Cutaneous Wound Healing Process via Activation of the PI3K/AKT, Wnt/β-catenin and NF-κB Signaling Pathways.
实验应用 : WB
种属反应 : Human
Wang CY et al. CLINICS ONCOL 2016; 1:1015 Over-Expression of Cofilin-1 Suppressed Mobility of Lung Cancer Cells is Associated with Down-Regulation of SNAIL-1 and Induction of Let-7.
实验应用 : WB
种属反应 : Human
Wang TH et al. Oncotarget 2015; 6 (27):23342-57 Long non-coding RNA AOC4P suppresses hepatocellular carcinoma metastasis by enhancing vimentin degradation and inhibiting epithelial-mesenchymal transition.
实验应用 : WB
种属反应 : Human
Chen CY et al. Oncotarget 2015; 6 (29):26946-59 Targeting annexin A2 reduces tumorigenesis and therapeutic resistance of nasopharyngeal carcinoma.
实验应用 : WB
种属反应 : Human
Agliarulo I et al. Biochim Biophys Acta 2015; 1853 (10 Pt A):244958 TRAP1 controls cell migration of cancer cells in metabolic stress conditions: Correlations with AKT/p70S6K pathways.
实验应用 : WB
种属反应 : Human
Szu-Ting Lin et al. Cellular and Molecular Life Sciences 2015; 72 (12):2395-409 pgrmc1 contributes to *-induced chemoresistance in mes-sa uterine sarcoma.
实验应用 : WB
种属反应 : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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