GeneTex中国官方网站
  • China

SNAI1抗体

KO/KD Comparison Orthogonal Overexpression
Anti-SNAI1 antibody used in Western Blot (WB). GTX125918
Anti-SNAI1 antibody used in Western Blot (WB). GTX125918
Anti-SNAI1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125918
Anti-SNAI1 antibody used in Western Blot (WB). GTX125918
Anti-SNAI1 antibody used in IHC (Paraffin sections) (IHC-P). GTX125918
Anti-SNAI1 antibody used in Western Blot (WB). GTX125918
Anti-SNAI1 antibody used in Western Blot (WB). GTX125918
Anti-SNAI1 antibody used in Western Blot (WB). GTX125918
Anti-SNAI1 antibody used in IHC (Paraffin sections) (IHC-P). GTX125918
Anti-SNAI1 antibody used in Western Blot (WB). GTX125918

目录号 GTX125918

文献引用 26

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 SNAI1 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

29 kDa. ( Note )

阳性对照

Hela(1 ng/ml TGF-beta and 300 ng/ml of EGF for 2 hr) , HeLa (starvation for 16 hr 20 ng/ml TGF-beta and 300 ng/ml EGF treatment for 30 min) , SK-N-SH , IMR-32 , SK-N-AS

预测种属反应

Bovine(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.68 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human SNAI1. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2885614

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

snail family transcriptional repressor 1 , SLUGH2 , SNA , SNAH , SNAIL , SNAIL1 , dJ710H13.1

细胞定位

Nucleus , Cytoplasm

功能与背景

The Drosophila embryonic protein snail is a zinc finger transcriptional repressor which downregulates the expression of ectodermal genes within the mesoderm. The nuclear protein encoded by this gene is structurally similar to the Drosophila snail protein, and is also thought to be critical for mesoderm formation in the developing embryo. At least two variants of a similar processed pseudogene have been found on chromosome 2. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-SNAI1 antibody used in Western Blot (WB). GTX125918

GTX125918 WB Image

Comparison Orthogonal

Untreated (–) and treated (+) HeLa whole cell extracts (50 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with SNAI1 antibody (GTX125918) diluted at 1:1000 and competitor's antibody (#3879) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-SNAI1 antibody used in Western Blot (WB). GTX125918

GTX125918 WB Image

KO/KD

Wild-type (WT) and SNAI1 knockout (KO) HeLa cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody (GTX125918) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-SNAI1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX125918

GTX125918 ICC/IF Image

SNAI1 antibody detects SNAI1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SNAI1 protein stained by SNAI1 antibody (GTX125918) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-SNAI1 antibody used in Western Blot (WB). GTX125918

GTX125918 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody (GTX125918) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-SNAI1 antibody used in IHC (Paraffin sections) (IHC-P). GTX125918

GTX125918 IHC-P Image

SNAI1 antibody detects SNAI1 protein at nucleus and cytosol on human breast carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
SNAI1 antibody (GTX125918) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-SNAI1 antibody used in Western Blot (WB). GTX125918

GTX125918 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody (GTX125918) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SNAI1 antibody used in Western Blot (WB). GTX125918

GTX125918 WB Image

Orthogonal

Untreated (–) and treated (+) HeLa whole cell extract (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody (GTX125918) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SNAI1 antibody used in Western Blot (WB). GTX125918

GTX125918 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SNAI1 antibody (GTX125918) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-SNAI1 antibody used in IHC (Paraffin sections) (IHC-P). GTX125918

GTX125918 IHC-P Image

Orthogonal

The data was published in the journal Oncotarget in 2016.PMID: 26840566

Anti-SNAI1 antibody used in Western Blot (WB). GTX125918

GTX125918 WB Image

Orthogonal

The data was published in the journal Oncotarget in 2016.PMID: 26840566

文献引用

Application Reference

TH Chang et al. Research Square 2023; The CCL2-CCR2 axis contributes to acquired osimertinib resistance in lung cancer by upregulating Zeb1 expression
实验应用 : WB
Wu WS et al. Am J Cancer Res 2023; 13 (10) : 4903-4917 Preclinical trial of targeting the Hic-5-mediated pathway to prevent the progression of hepatocellular carcinoma.
Chen WC et al. Cancers (Basel) 2023; 15 (15) Elafin as a Prognostic Marker in Esophageal Squamous Cell Carcinoma: A Pilot Study Using Three-Dimensional Imaging and Genomic Profiling.
TH Hung et al. Biomed J 2023; : 100612 High expression of embryonic stem cell marker SSEA3 confers poor prognosis and promotes epithelial mesenchymal transition in hepatocellular carcinoma
实验应用 : WB
种属反应 : Human
Wang L et al. Bioengineered 2022; 13 (5) : 12941-12954 Stearoyl-CoA desaturase 1 regulates malignant progression of cervical cancer cells.
显示更多 +
我要回报文献

产品评论

目前没有使用 SNAI1 antibody (GTX125918) 的产品评论被收录。快发表第一篇产品评论吧！
我要评论产品
Application Reference
TH Chang et al. Research Square 2023; The CCL2-CCR2 axis contributes to acquired osimertinib resistance in lung cancer by upregulating Zeb1 expression
实验应用 : WB
Wu WS et al. Am J Cancer Res 2023; 13 (10):4903-4917 Preclinical trial of targeting the Hic-5-mediated pathway to prevent the progression of hepatocellular carcinoma.
Chen WC et al. Cancers (Basel) 2023; 15 (15) Elafin as a Prognostic Marker in Esophageal Squamous Cell Carcinoma: A Pilot Study Using Three-Dimensional Imaging and Genomic Profiling.
TH Hung et al. Biomed J 2023;:100612 High expression of embryonic stem cell marker SSEA3 confers poor prognosis and promotes epithelial mesenchymal transition in hepatocellular carcinoma
实验应用 : WB
种属反应 : Human
Wang L et al. Bioengineered 2022; 13 (5):12941-12954 Stearoyl-CoA desaturase 1 regulates malignant progression of cervical cancer cells.
Wang LT et al. Cancer Lett 2021; 520:160-171 Phosphorylation of intestine-specific homeobox by ERK1 modulates oncogenic activity and sorafenib resistance.
实验应用 : WB
种属反应 : Mouse
Lv F et al. Eur J Pharmacol 2021; 900:174069 Eriodictyol inhibits glioblastoma migration and invasion by reversing EMT via downregulation of the P38 MAPK/GSK-3β/ZEB1 pathway.
Jao TM et al. Cancer Lett 2021; 499:290-300 PCDH10 exerts tumor-suppressor functions through modulation of EGFR/AKT axis in colorectal cancer.
实验应用 : WB
种属反应 : Human
Kim BN et al. Sci Rep 2020; 10 (1):10597 TGF-β induced EMT and stemness characteristics are associated with epigenetic regulation in lung cancer.
Lee KY et al. Cells 2020; 10 (1) Upregulation of CD109 Promotes the Epithelial-to-Mesenchymal Transition and Stemness Properties of Lung Adenocarcinomas via Activation of the Hippo-YAP Signaling.
实验应用 : WB
种属反应 : Human
Kuo CH et al. Cell Biochem Funct 2020; (Epub) Biomarker discovery in highly invasive lung cancer cell through proteomics approaches.
实验应用 : WB
种属反应 : Human
Huang HY et al. J Cell Mol Med 2020; Progesterone receptor membrane component 1 is involved in oral cancer cell metastasis.
实验应用 : WB
种属反应 : Human
Harada H et al. Oncol Lett 2019; 17 (1):578-586 Carcinosarcoma of the esophagus: A report of 6 cases associated with zinc finger E-box-binding homeobox 1 expression.
Bacci L et al. Int J Mol Sci 2019; 20 (16) H19-Dependent Transcriptional Regulation of β3 and β4 Integrins Upon Estrogen and Hypoxia Favors Metastatic Potential in Prostate Cancer.
实验应用 : WB
种属反应 : Human
Liu YP et al. Cancer Sci 2018; 109 (10):3105-3114 Human immunodeficiency virus Tat-TIP30 interaction promotes metastasis by enhancing the nuclear translocation of Snail in lung cancer cell lines.
Hiroki Harada et al. Oncology Letters 2018; 17 (1):578-586 Carcinosarcoma of the esophagus: A report of 6 cases associated with zinc finger E-box-binding homeobox 1 expression.
实验应用 : IHC-P
Lai YJ et al. J Cell Physiol 2018; (Epub) CSC-3436 inhibits TWIST-induced epithelial-mesenchymal transition via the suppression of Twist/Bmi1/Akt pathway in head and neck squamous cell carcinoma.
实验应用 : WB
种属反应 : Human
Zhenzhen L et al. Clinical Science 2018; 132 (21):2339-2355 MKP2 inhibits TGF-β1-induced epithelial-to-mesenchymal transition in renal tubular epithelial cells through a JNK-dependent pathway.
实验应用 : WB
种属反应 : Rat
Micati DJ et al. Andrology 2018; (Epub) Differential expression profiles of conserved Snail transcription factors in the mouse testis.
实验应用 : IHC-P
种属反应 : Mouse
Lee J et al. J Biol Chem 2018; (Epub) Fibroblast growth factor 2 induces proliferation and fibrosis via SNAI1-mediated activation of CDK2 and ZEB1 in corneal endothelium.
种属反应 : Human
Kuo WY et al. Theranostics 2017; 7 (3):647 stat3/nf-κb-regulated lentiviral tk/gcv suicide gene therapy for *-resistant triple-negative breast cancer.
实验应用 : WB
种属反应 : Human
Yin K et al. Oncotarget 2016; 7 (17):24537-48 MiR-206 suppresses epithelial mesenchymal transition by targeting TGF-β signaling in estrogen receptor positive breast cancer cells.
实验应用 : WB
种属反应 : Human
Chung IH et al. Oncotarget 2016; 7 (10):11113-26 Overexpression of lipocalin 2 in human cervical cancer enhances tumor invasion.
实验应用 : IHC-P
种属反应 : Human
Ling HH et al. Exp Cell Res 2016; Elevation of YAP promotes the epithelial-mesenchymal transition and tumor aggressiveness in colorectal cancer.
实验应用 : WB
种属反应 : Human
Horvay K et al. EMBO J 2015; 34 (10):1319-35 Snai1 regulates cell lineage allocation and stem cell maintenance in the mouse intestinal epithelium.
实验应用 : IHC
Wai-Theng Lee et al. Food and Chemical Toxicology 2015; 78:33-41 Antroquinonol from Antrodia Camphorata suppresses breast tumor migration/invasion through inhibiting ERK-AP-1- and AKT-NF-κBdependent MMP-9 and epithelial-mesenchymal transition expressions
实验应用 : WB
种属反应 : Human
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
联系经销商
欣博盛生物科技有限公司
欣博盛生物科技有限公司
(全国热线) 4006-800-892 / (深圳) 0755-26755892 / (北京) 010-88594029 / (上海) 021-34613729 / (广州) 18024516375 / (香港) +852-69410778
http://www.neobioscience.com
genetex@neobioscience.com