产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fragmemt of human SOD1
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
superoxide dismutase 1 , ALS , ALS1 , HEL-S-44 , IPOA , SOD , hSod1 , homodimer
细胞定位
Cytoplasm,Mitochondrion,Nucleus
功能与背景
The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
![Anti-SOD1 antibody [HL2033] used in Western Blot (WB). GTX637934](/templates/default/js/lightbox/images/loading.gif "Anti-SOD1 antibody [HL2033] used in Western Blot (WB). GTX637934")
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GTX637934 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody [HL2033] (GTX637934) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637934 WB Image
Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody [HL2033] (GTX637934) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637934 WB Image
Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody [HL2033] (GTX637934) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637934 WB Image
Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody [HL2033] (GTX637934) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637934 IHC-P Image
SOD1 antibody [HL2033] detects SOD1 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded dog cerebellum.SOD1 stained by SOD1 antibody [HL2033] (GTX637934) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637934 IHC-P Image
SOD1 antibody [HL2033] detects SOD1 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded cat brain.SOD1 stained by SOD1 antibody [HL2033] (GTX637934) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637934 IHC-P Image
SOD1 antibody [HL2033] detects SOD1 protein at cytoplasm and nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse prostate.SOD1 stained by SOD1 antibody [HL2033] (GTX637934) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637934 WB Image
Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with SOD1 antibody [HL2033] (GTX637934) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637934 ICC/IF Image
SOD1 antibody [HL2033] detects SOD1 protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SOD1 stained by SOD1 antibody [HL2033] (GTX637934) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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文献引用
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产品评论
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![Anti-SOD1 antibody [HL2033] used in Western Blot (WB). GTX637934](/upload/website/prouct_img/small/GTX637934/GTX637934_44977_20231117_WB_shRNA_watermark_23112021_344.webp "Anti-SOD1 antibody [HL2033] used in Western Blot (WB). GTX637934")
![Anti-SOD1 antibody [HL2033] used in Western Blot (WB). GTX637934](/upload/website/prouct_img/small/GTX637934/GTX637934_44977_20230616_WB_D_23062019_113.webp "Anti-SOD1 antibody [HL2033] used in Western Blot (WB). GTX637934")
![Anti-SOD1 antibody [HL2033] used in Western Blot (WB). GTX637934](/upload/website/prouct_img/small/GTX637934/GTX637934_44977_20230616_WB_Z_23062019_495.webp "Anti-SOD1 antibody [HL2033] used in Western Blot (WB). GTX637934")
![Anti-SOD1 antibody [HL2033] used in Western Blot (WB). GTX637934](/upload/website/prouct_img/small/GTX637934/GTX637934_44977_20230317_WB_23032022_792.webp "Anti-SOD1 antibody [HL2033] used in Western Blot (WB). GTX637934")
![Anti-SOD1 antibody [HL2033] used in IHC (Paraffin sections) (IHC-P). GTX637934](/upload/website/prouct_img/small/GTX637934/GTX637934_44977_20230721_IHC-P_Dog_23073119_543.webp "Anti-SOD1 antibody [HL2033] used in IHC (Paraffin sections) (IHC-P). GTX637934")
![Anti-SOD1 antibody [HL2033] used in IHC (Paraffin sections) (IHC-P). GTX637934](/upload/website/prouct_img/small/GTX637934/GTX637934_44977_20230721_IHC-P_Cat_23073119_195.webp "Anti-SOD1 antibody [HL2033] used in IHC (Paraffin sections) (IHC-P). GTX637934")
![Anti-SOD1 antibody [HL2033] used in IHC (Paraffin sections) (IHC-P). GTX637934](/upload/website/prouct_img/small/GTX637934/GTX637934_T-44879_20230106_IHC-P_M_23010400_601.webp "Anti-SOD1 antibody [HL2033] used in IHC (Paraffin sections) (IHC-P). GTX637934")
![Anti-SOD1 antibody [HL2033] used in Western Blot (WB). GTX637934](/upload/website/prouct_img/small/GTX637934/GTX637934_T-44879_20221202_WB_M_R_23010400_510.webp "Anti-SOD1 antibody [HL2033] used in Western Blot (WB). GTX637934")
![Anti-SOD1 antibody [HL2033] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637934](/upload/website/prouct_img/small/GTX637934/GTX637934_T-44879_20230203_ICC_IF_23021401_440.webp "Anti-SOD1 antibody [HL2033] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637934")
![Anti-SOD1 antibody [HL2033] used in Western Blot (WB). GTX637934](/upload/website/prouct_img/normal/GTX637934/GTX637934_44977_20231117_WB_shRNA_watermark_23112021_344.webp "Anti-SOD1 antibody [HL2033] used in Western Blot (WB). GTX637934")
![Anti-SOD1 antibody [HL2033] used in Western Blot (WB). GTX637934](/upload/website/prouct_img/normal/GTX637934/GTX637934_44977_20230616_WB_D_23062019_113.webp "Anti-SOD1 antibody [HL2033] used in Western Blot (WB). GTX637934")
![Anti-SOD1 antibody [HL2033] used in Western Blot (WB). GTX637934](/upload/website/prouct_img/normal/GTX637934/GTX637934_44977_20230616_WB_Z_23062019_495.webp "Anti-SOD1 antibody [HL2033] used in Western Blot (WB). GTX637934")
![Anti-SOD1 antibody [HL2033] used in Western Blot (WB). GTX637934](/upload/website/prouct_img/normal/GTX637934/GTX637934_44977_20230317_WB_23032022_792.webp "Anti-SOD1 antibody [HL2033] used in Western Blot (WB). GTX637934")
![Anti-SOD1 antibody [HL2033] used in IHC (Paraffin sections) (IHC-P). GTX637934](/upload/website/prouct_img/normal/GTX637934/GTX637934_44977_20230721_IHC-P_Dog_23073119_543.webp "Anti-SOD1 antibody [HL2033] used in IHC (Paraffin sections) (IHC-P). GTX637934")
![Anti-SOD1 antibody [HL2033] used in IHC (Paraffin sections) (IHC-P). GTX637934](/upload/website/prouct_img/normal/GTX637934/GTX637934_44977_20230721_IHC-P_Cat_23073119_195.webp "Anti-SOD1 antibody [HL2033] used in IHC (Paraffin sections) (IHC-P). GTX637934")
![Anti-SOD1 antibody [HL2033] used in IHC (Paraffin sections) (IHC-P). GTX637934](/upload/website/prouct_img/normal/GTX637934/GTX637934_T-44879_20230106_IHC-P_M_23010400_601.webp "Anti-SOD1 antibody [HL2033] used in IHC (Paraffin sections) (IHC-P). GTX637934")
![Anti-SOD1 antibody [HL2033] used in Western Blot (WB). GTX637934](/upload/website/prouct_img/normal/GTX637934/GTX637934_T-44879_20221202_WB_M_R_23010400_510.webp "Anti-SOD1 antibody [HL2033] used in Western Blot (WB). GTX637934")
![Anti-SOD1 antibody [HL2033] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637934](/upload/website/prouct_img/normal/GTX637934/GTX637934_T-44879_20230203_ICC_IF_23021401_440.webp "Anti-SOD1 antibody [HL2033] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637934")
