产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 0.01-0.03μg/ml |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
The peptide used to generate this antibody is available for purchase (GTX89049-PEP).
预测种属反应
Rat, Dog(>80% identity)
产品属性
形式
Liquid
存储溶液
TBS, 0.5% BSA
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Peptide with sequence C-SRKHGGPKDEERH, from the internal region of the protein sequence according to NP_000445.1.
纯化方式
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
superoxide dismutase 1 , ALS , ALS1 , HEL-S-44 , IPOA , SOD , hSod1 , homodimer
细胞定位
Cytoplasm , Mitochondrion , Nucleus
功能与背景
The protein encoded by this gene binds copper and zinc ions and is one of two isozymes responsible for destroying free superoxide radicals in the body. The encoded isozyme is a soluble cytoplasmic protein, acting as a homodimer to convert naturally-occuring but harmful superoxide radicals to molecular oxygen and hydrogen peroxide. The other isozyme is a mitochondrial protein. Mutations in this gene have been implicated as causes of familial amyotrophic lateral sclerosis. Rare transcript variants have been reported for this gene. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
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GTX89049 ELISA Image
ELISA analysis of SOD1 protein using GTX89049 SOD1 antibody, Internal.
Capture : GTX100554 (5μg/ml)
Detection : GTX89049 (1.5μg/ml)
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GTX89049 WB Image
WB analysis of mouse brain lysate using GTX89049 SOD1 antibody, Internal.
Dilution : 0.02μg/ml
Loading : 35μg protein in RIPA buffer
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GTX89049 WB Image
WB analysis of NIH-3T3 (A), HEK293 (B), HepG2 (C) and MCF7 (D) lysates using GTX89049 SOD1 antibody, Internal.
Dilution : 0.01μg/ml
Loading : 35μg protein in RIPA buffer
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