产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
预测种属反应
Rat, Zebrafish, Chicken, Pig, Xenopus tropicalis, Chimpanzee, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
2 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Carrier-protein conjugated synthetic peptide surrounding acetyl Lys68 of human SOD2. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
superoxide dismutase 2 , IPO-B , IPOB , MNSOD , MVCD6 , Mn-SOD
细胞定位
Mitochondrion matrix
功能与背景
This gene is a member of the iron/manganese superoxide dismutase family. It encodes a mitochondrial protein that forms a homotetramer and binds one manganese ion per subunit. This protein binds to the superoxide byproducts of oxidative phosphorylation and converts them to hydrogen peroxide and diatomic oxygen. Mutations in this gene have been associated with idiopathic cardiomyopathy (IDC), premature aging, sporadic motor neuron disease, and cancer. Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been identified on chromosome 1. [provided by RefSeq, Apr 2016]
数据库
研究领域
產品图片
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GTX636934 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SOD2 (Acetyl Lys68) antibody [HL1460] (GTX636934) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636934 WB Image
Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOD2 (Acetyl Lys68) antibody [HL1460] (GTX636934) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636934 Dot Image
Dotblot analysis of anti-SOD2 (Acetyl Lys68) antibody [HL1460] with peptide samples. Peptide samples were spotted onto positively charged nylon membrane and blotted with SOD2 (Acetyl Lys68) antibody [HL1460] (GTX636934) varied dilution.
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文献引用
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