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SOX2抗体 [GT1876]

KO/KD Comparison Orthogonal
Anti-SOX2 antibody [GT1876] used in IHC (Frozen sections) (IHC-Fr). GTX627404
Anti-SOX2 antibody [GT1876] used in Western Blot (WB). GTX627404
Anti-SOX2 antibody [GT1876] used in Western Blot (WB). GTX627404
Anti-SOX2 antibody [GT1876] used in Western Blot (WB). GTX627404
Anti-SOX2 antibody [GT1876] used in IHC (Frozen sections) (IHC-Fr). GTX627404
Anti-SOX2 antibody [GT1876] used in Western Blot (WB). GTX627404
Anti-SOX2 antibody [GT1876] used in Western Blot (WB). GTX627404
Anti-SOX2 antibody [GT1876] used in Immunoprecipitation (IP). GTX627404
Anti-SOX2 antibody [GT1876] used in IHC (Frozen sections) (IHC-Fr). GTX627404
Anti-SOX2 antibody [GT1876] used in Flow cytometry (FACS). GTX627404
Anti-SOX2 antibody [GT1876] used in Western Blot (WB). GTX627404
Anti-SOX2 antibody [GT1876] used in Western Blot (WB). GTX627404
Anti-SOX2 antibody [GT1876] used in Western Blot (WB). GTX627404
Anti-SOX2 antibody [GT1876] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX627404

目录号 GTX627404

文献引用 14
2

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 GT1876

同种型

 IgG1

实验应用

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP

种属反应

 Human, Mouse, Rat, Zebrafish
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 SOX2 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:10000
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr 1:100-1:1000
FACS 1:50-1:200
IP 1:100-1:500

Note :

IHC-Fr
The use of GTX627404 for zebrafish is based on WB testing. For IHC or IHC whole mount using zebrafish samples, we recommend GTX124477.
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

34 kDa. ( Note )

阳性对照

human ESC , zebrafish eye , NT2D1 , PC-3 , U87-MG , SK-N-SH

预测种属反应

Sheep, Bovine, Cat, Chicken, Pig, Japanese Medaka, Xenopus tropicalis, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human SOX2. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein G.

偶联

Unconjugated

RRID

AB_11162865

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

SRY-box 2 , ANOP3 , MCOPS3

细胞定位

Nucleus

功能与背景

This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq]

数据库

研究领域

產品图片

Anti-SOX2 antibody [GT1876] used in IHC (Frozen sections) (IHC-Fr). GTX627404

GTX627404 IHC-Fr Image

SOX2 antibody [GT1876] detects SOX2 protein expression at nucleus by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Red: SOX2 protein stained by SOX2 antibody [GT1876] (GTX627404) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-SOX2 antibody [GT1876] used in Western Blot (WB). GTX627404

GTX627404 WB Image

Comparison

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membranes were blotted with SOX2 antibody [GT1876] (GTX627404) diluted at 1:1000 and competitor's antibody (sc-17320) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-SOX2 antibody [GT1876] used in Western Blot (WB). GTX627404

GTX627404 WB Image

Sample (30 ug of whole cell lysate)
A: NT2D1
B: PC-3
C: U87-MG
D: SK-N-SH
10% SDS PAGE
GTX627404 diluted at 1:5000

Anti-SOX2 antibody [GT1876] used in Western Blot (WB). GTX627404

GTX627404 WB Image

KO/KD

Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SOX2 antibody [GT1876] (GTX627404) diluted at 1:500.

Anti-SOX2 antibody [GT1876] used in IHC (Frozen sections) (IHC-Fr). GTX627404

GTX627404 IHC-Fr Image

SOX2 antibody [GT1876] detects SOX2 protein by immunohistochemical analysis.
Sample: Frozen sectioned adult mouse retina.
Green: SOX2 protein stained by SOX2 antibody [GT1876] (GTX627404) diluted at 1:250.
Red: PKC alpha protein stained by PKC alpha antibody [GT1876] (GTX130453) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-SOX2 antibody [GT1876] used in Western Blot (WB). GTX627404

GTX627404 WB Image

SOX2 antibody [GT1876] detects SOX2 protein by Western blot analysis.
A. 30 μg zebrafish eye lysate/extract
10 % SDS-PAGE
SOX2 antibody [GT1876] (GTX627404) dilution: 1:500

Anti-SOX2 antibody [GT1876] used in Western Blot (WB). GTX627404

GTX627404 WB Image

SOX2 antibody [GT1876] detects SOX2 protein by Western blot analysis.
A. 30 μg human ESC whole cell lysate/extract
10 % SDS-PAGE
SOX2 antibody [GT1876] (GTX627404) dilution: 1:5000

Anti-SOX2 antibody [GT1876] used in Immunoprecipitation (IP). GTX627404

GTX627404 IP Image

Immunoprecipitation of SOX2 protein from NT2D1 whole cell extracts using 5 μg of SOX2 antibody [GT1876] (GTX627404) or SOX2 antibody [N1C3] (GTX101057).
Western blot analysis was performed using SOX2 antibody [GT1876] (GTX627404) diluted at 1:500.
EasyBlot anti-Mouse IgG (GTX221667-01) was used as a secondary reagent.

Anti-SOX2 antibody [GT1876] used in IHC (Frozen sections) (IHC-Fr). GTX627404

GTX627404 IHC-Fr Image

Sox2 antibodies detects Sox2 proteins on embryonic mouse brain by immunohistochemical analysis.
Sample: Frozen section of embryonic mouse brain (mE18.5).
Green: GFAP antibody (GTX100850) diluted at 1:500.
Red: Sox2 antibody [GT1876] (GTX627404) diluted at 1:500.

Anti-SOX2 antibody [GT1876] used in Flow cytometry (FACS). GTX627404

GTX627404 FACS Image

SOX2 antibody [GT1876] detects SOX2 protein by flow cytomertry analysis. Sample: Human embryonic stem cells Black: Isotype control dilution: 1:50 Green: SOX2 antibody [GT1876] dilution: 1:50

Anti-SOX2 antibody [GT1876] used in Western Blot (WB). GTX627404

GTX627404 WB Image

Orthogonal

The data was published in the 2020 in Sci Rep. PMID: 32398775

Anti-SOX2 antibody [GT1876] used in Western Blot (WB). GTX627404

GTX627404 WB Image

The data was published in the journal Sci Rep in 2016. PMID: 27344963

Anti-SOX2 antibody [GT1876] used in Western Blot (WB). GTX627404

GTX627404 WB Image

The data was published in the journal Sci Rep in 2016. PMID: 27344963

Anti-SOX2 antibody [GT1876] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX627404

GTX627404 ICC/IF Image

The data was published in the journal Int J Biol Sci in 2019.PMID: 31595152

文献引用

Application Reference

Ma L et al. Cell Death Dis 2024; 15 (3) : 229 METTL3-dependent m(6)A modification of PSEN1 mRNA regulates craniofacial development through the Wnt/β-catenin signaling pathway.
Mashiko T et al. Cells 2021; 10 (3) Selective Proliferation of Highly Functional Adipose-Derived Stem Cells in Microgravity Culture with Stirred Microspheres.
实验应用 : ICC/IF
种属反应 : Human
Zhang L et al. Cancer Lett 2021; 500 : 87-97 A novel integrated system using patient-derived glioma cerebral organoids and xenografts for disease modeling and drug screening.
实验应用 : IHC-P
种属反应 : Human
Lee KY et al. Cells 2020; 10 (1) Upregulation of CD109 Promotes the Epithelial-to-Mesenchymal Transition and Stemness Properties of Lung Adenocarcinomas via Activation of the Hippo-YAP Signaling.
实验应用 : WB
种属反应 : Human
Ma L et al. J Cell Mol Med 2020; (Epub) Identification of novel susceptibility loci for non-syndromic cleft lip with or without cleft palate.
实验应用 : WB
种属反应 : Zebrafish
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产品评论

SOX2 antibody [GT1876] 目录号 GTX627404

Rating
( Average 4 based on 2 users reviews)
实验应用
IHC (Paraffin sections)(IHC-P)
( Average 4 based on 1 users reviews)
Flow cytometry(FACS)
( Average 4 based on 1 users reviews)
Anti-SOX2 antibody [GT1876] used in IHC (Paraffin sections) (IHC-P). GTX627404
Date : Anonymous submitted on 25-Aug-2021
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : mice brain, hippocampus, 5~10μm slide
Blocking : 3% BSA in PBST, RT, 1Hr
Fixation : 4% formalin
Permeabilization /Antigen retrieval : 10mM Citrate Buffer, 0.05% Tween-20, pH6
Primary Antibody : 1:100, 4°C, > 12Hr
Anti-SOX2 antibody [GT1876] used in Flow cytometry (FACS). GTX627404
Date : Anonymous submitted on 11-Mar-2013
Score :
Application Tested : FACS
Sample Species : Hu
Sample : hES cells
Blocking : 2% serum, 4°C, 0.5Hr
Fixation : 70% ethanol
Primary Antibody : 1:50, 4°C, 0.5Hr
Figure Note : Black : Isotype control, Green : SOX2 antibody
我要评论产品
Application Reference
Ma L et al. Cell Death Dis 2024; 15 (3):229 METTL3-dependent m(6)A modification of PSEN1 mRNA regulates craniofacial development through the Wnt/β-catenin signaling pathway.
Mashiko T et al. Cells 2021; 10 (3) Selective Proliferation of Highly Functional Adipose-Derived Stem Cells in Microgravity Culture with Stirred Microspheres.
实验应用 : ICC/IF
种属反应 : Human
Zhang L et al. Cancer Lett 2021; 500:87-97 A novel integrated system using patient-derived glioma cerebral organoids and xenografts for disease modeling and drug screening.
实验应用 : IHC-P
种属反应 : Human
Lee KY et al. Cells 2020; 10 (1) Upregulation of CD109 Promotes the Epithelial-to-Mesenchymal Transition and Stemness Properties of Lung Adenocarcinomas via Activation of the Hippo-YAP Signaling.
实验应用 : WB
种属反应 : Human
Ma L et al. J Cell Mol Med 2020; (Epub) Identification of novel susceptibility loci for non-syndromic cleft lip with or without cleft palate.
实验应用 : WB
种属反应 : Zebrafish
Andreucci E et al. J Mol Med (Berl) 2020; 98 (10):1431-1446 The acidic tumor microenvironment drives a stem-like phenotype in melanoma cells.
实验应用 : FACS
种属反应 : Human
Aminuddin A et al. Sci Rep 2020; 10 (1):7885
实验应用 : WB
种属反应 : Human
Peng L et al. Int J Biol Sci 2019; 15 (11):2340-2349 Generation of Stable Induced Pluripotent Stem-like Cells from Adult Zebra Fish Fibroblasts.
实验应用 : ICC/IF
种属反应 : Zebrafish
Liangyue Peng et al. Int J Biol Sci 2019; 15 (11):2340-2349 Generation of Stable Induced Pluripotent Stem-like Cells from Adult Zebra Fish Fibroblasts.
实验应用 : ICC/IF
种属反应 : Zebrafish
Lin YH et al. Endocr Relat Cancer 2018; 25 (12):967-979 Thyroid hormone negatively regulates tumorigenesis through suppression of BC200.
实验应用 : WB
种属反应 : Human
Chuang HM et al. Int J Mol Sci 2018; 19 (10) Non-Canonical Regulation of Type I Collagen through Promoter Binding of SOX2 and Its Contribution to Ameliorating Pulmonary Fibrosis by Butylidenephthalide.
实验应用 : WB
种属反应 : Human
Chang KY et al. Biochem Biophys Res Commun 2017; 493 (1):14-19 Stress stimuli induce cancer-stemness gene expression via Sp1 activation leading to therapeutic resistance in glioblastoma.
实验应用 : WB
种属反应 : Human
Li HK et al. Sci Rep 2016; 6:28637 DDX3 Represses Stemness by Epigenetically Modulating Tumor-suppressive miRNAs in Hepatocellular Carcinoma.
实验应用 : WB
种属反应 : Human
Huang TT et al. Oncotarget 2015; 6 (20):18012-26 The reciprocal regulation loop of Notch2 pathway and miR-23b in controlling gastric carcinogenesis.
实验应用 : WB
种属反应 : Human
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产品包装 报价 (¥)
¥ 4000
¥ 1700
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