产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
Assay dependent |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fragment of human SOX2.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
SRY-box 2 , ANOP3 , MCOPS3
细胞定位
Nucleus
功能与背景
This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
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GTX636505 WB Image
Non-transfected (–) and transfected (+) NT2D1 whole cell extract (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody [HL1193] (GTX636505) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636505 WB Image
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membranes were blotted with SOX2 antibody [HL1193] (GTX636505) diluted at 1:1000 and competitor's antibody (sc-17320) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX636505 WB Image
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody [HL1193] (GTX636505) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636505 WB Image
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody [HL1193] (GTX636505) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636505 WB Image
Mouse ESC extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody [HL1193] (GTX636505) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636505 IHC-P Image
SOX2 antibody detects SOX2 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.SOX2 stained by SOX2 antibody (GTX636505) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636505 IHC-P Image
SOX2 antibody detects SOX2 protein by immunohistochemical analysis.Sample: Paraffin-embedded rat tissues.SOX2 stained by SOX2 antibody (GTX636505) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636505 ICC/IF Image
SOX2 antibody [HL1193] detects SOX2 protein at nucleus by immunofluorescent analysis.Sample: DIV10 rat E18 primary hippocampal neuron cells cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SOX2 stained by SOX2 antibody [HL1193] (GTX636505) diluted at 1:500.Red: Tau, a Tau marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
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