产品说明
摘要
SOX2 antibody detects SOX2 protein, a basic helix-loop-helix transcription factor (predicted molecular weight of 34 kDa). SOX2 is an indispensable factor for regulating embryonic development and in the generation of inducible pluripotent stem (iPS) cells. It is also expressed in neural stem and progenitor cells. Dysregulation of SOX2 is associated with tumorigenesis.
产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:5000-1:20000 |
1:100-1:1000 |
1:100-1:1000 |
1:100-1:1000 |
1:50-1:200 |
1:100-1:500 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
Mouse ESC , hESC , NT2D1 , NT2D1
预测种属反应
Zebrafish, Sheep, Bovine, Cat, Chicken, Pig, Japanese Medaka, Xenopus tropicalis, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 1% BSA, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.15 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human SOX2. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2038021
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
SRY-box 2 , ANOP3 , MCOPS3
细胞定位
Nucleus
功能与背景
This intronless gene encodes a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. The product of this gene is required for stem-cell maintenance in the central nervous system, and also regulates gene expression in the stomach. Mutations in this gene have been associated with optic nerve hypoplasia and with syndromic microphthalmia, a severe form of structural eye malformation. This gene lies within an intron of another gene called SOX2 overlapping transcript (SOX2OT). [provided by RefSeq]
数据库
研究领域
產品图片
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GTX101507 WB Image
Non-transfected (–) and transfected (+) NT2D1 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SOX2 antibody [N1C3] (GTX101507) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101507 IHC-P Image
SOX2 antibody [N1C3] detects SOX2 protein at nucleus in mouse esophagus by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. SOX2 antibody [N1C3] (GTX101507) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX101507 WB Image
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membranes were blotted with SOX2 antibody [N1C3] (GTX101507) diluted at 1:10000 and competitor's antibody (sc-17320) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX101507 WB Image
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membranes were blotted with SOX2 antibody [N1C3] (GTX101507) diluted at 1:1000 and competitor's antibody (sc-17320) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX101507 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using SOX2(GTX101507) antibody at 1:160 dilution.
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GTX101507 IHC-Fr Image
SOX2 antibody [N1C3] detects SOX2 protein at nucleus by immunohistochemical analysis. Sample: Frozen sectioned E13.5 rat brain. Green: SOX2 protein stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:250. Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).
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GTX101507 IHC-Fr Image
SOX2 antibody [N1C3] detects SOX2 protein expression by immunohistochemical analysis. Sample: Frozen-sectioned adult mouse hippocampus. Green: SOX2 protein stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:250. Blue: Fluoroshield with DAPI (GTX30920).
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GTX101507 ICC/IF Image
SOX2 antibody [N1C3] detects SOX2 protein at nucleus by immunofluorescent analysis.Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SOX2 stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:500.Red: phalloidin, a cytoskeleton marker, diluted at 1:100.Scale bar= 10 μm.
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GTX101507 FACS Image
Flow cytometry on human embryonic stem cells, staining with SOX2 (GTX101507)antibody at 1:100 dilution(purple) or rabbit IgG (black).
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GTX101507 IHC-P Image
Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SOX2(GTX101507) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101507 WB Image
SOX2 antibody detects SOX2 protein by Western blot analysis. Whole cell extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody (GTX101507) at a dilution of 1:2500.
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GTX101507 IHC-P Image
SOX2 antibody [N1C3] detects SOX2 protein at nucleus on rat brain stem by immunohistochemical analysis. Sample: Paraffin-embedded rat brain stem. SOX2 antibody [N1C3] (GTX101507) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101507 WB Image
Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody [N1C3] (GTX101507) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101507 IHC-P Image
SOX2 antibody [N1C3] detects SOX2 protein at nucleus in human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. SOX2 antibody [N1C3] (GTX101507) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX101507 IP Image
Immunoprecipitation of SOX2 protein from NT2D1 whole cell extracts using 5 μg of SOX2 antibody [N1C3] (GTX101507) or SOX2 antibody [GT1876] (GTX627404). Western blot analysis was performed using SOX2 antibody [N1C3] (GTX101507) diluted at 1:500. EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX101507 IP Image
Immunoprecipitation of SOX2 protein from NT2D1 whole cell extracts using 5 μg of SOX2 antibody [N1C3] (GTX101507). Western blot analysis was performed using SOX2 antibody [N1C3] (GTX101507). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
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GTX101507 WB Image
SOX2 antibody detects SOX2 protein by western blot analysis. Mouse ESC lysate (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with SOX2 antibody (GTX101507) at a dilution of 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101507 IHC-Fr Image
SOX2 antibody [N1C3] detects SOX2 protein at nucleus by immunohistochemical analysis. Sample: Frozen sectioned adult mouse retina. Green: SOX2 protein stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:250. Red: Protein kinase C alpha staining. Blue: Fluoroshield with DAPI (GTX30920).
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GTX101507 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC, using SOX2(GTX101507) antibody at 1:80 dilution.
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GTX101507 IHC-P Image
SOX2 antibody [N1C3] detects SOX2 protein at nucleus in mouse fetal brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fetal brain. Green: SOX2 antibody [N1C3] (GTX101507) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04). Blue: Nuclear staining with Hoechst 33342.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX101507 IHC-P Image
SOX2 antibody [N1C3] detects SOX2 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human esophageal carcinoma.SOX2 stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX101507 IHC-P Image
SOX2 antibody [N1C3] detects SOX2 protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. SOX2 antibody [N1C3] (GTX101507) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101507 WB Image
The data was published in the journal Elife in 2017.PMID: 28537559
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GTX101507 WB Image
The data was published in the journal J Cancer in 2020.PMID: 32201526
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GTX101507 ICC/IF Image
The data was published in the journal J Cancer in 2020.PMID: 32201526
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GTX101507 WB Image
The data was published in the journal Sci Rep in 2016.PMID: 27226076
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GTX101507 IHC-P Image
The data was published in the journal Neural Plast in 2016.PMID: 28018679
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GTX101507 IHC-P Image
The data was published in the journal Neural Plast in 2016.PMID: 28018679
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GTX101507 WB Image
The data was published in the journal Elife in 2017.PMID: 28537559
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GTX101507 IHC-Fr Image
The data was published in the journal Dis Model Mech in 2018.PMID: 29208635
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GTX101507 ICC/IF Image
The data was published in the journal PLoS One in 2016. PMID: 27802323
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GTX101507 WB Image
The data was published in the journal Stem Cell Res Ther in 2013. PMID: 24423398
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GTX101507 ICC/IF Image
The data was published in the journal Nat Commun in 2018. PMID: 29973581
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文献引用
产品评论
SOX2 antibody [N1C3]
目录号 GTX101507
Rating
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( Average 3 based on 1 users reviews)
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实验应用
Western Blot(WB)
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( Average 3 based on 1 users reviews)
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Date :
Anonymous submitted on 18-Jul-2016
Application Tested :
WB
Sample Species :
Hu
Sample :
A549 cells, T24 cells
Amount used :
1* 10^5 cells
Blocking :
5% milk, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
条带说明 :
Lane 1: A549 cells, Lane 2: T24 cells.