产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500 - 1:2000 |
| 1:50 - 1:200 |
| 1:50 - 1:200 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS, 50% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
Batch dependent (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
A synthesized peptide derived from human SP1.
纯化方式
Purified by affinity chromatography
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
SP1,Sp1 transcription factor
细胞定位
Nucleus,Cytoplasm
功能与背景
The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014]
数据库
研究领域
產品图片
![Anti-SP1 antibody [GT1220] used in Western Blot (WB). GTX01528](/templates/default/js/lightbox/images/loading.gif "Anti-SP1 antibody [GT1220] used in Western Blot (WB). GTX01528")
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GTX01528 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody [GT1220] (GTX01528) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX01528 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody [GT1220] (GTX01528) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
The observed M.W. is based on the publication: PMID: 30463940
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GTX01528 IHC-P Image
IHC-P analysis of rat spleen tissue section using GTX01528 SP1 antibody [GT1220].
Dilution : 1:100
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GTX01528 ICC/IF Image
ICC/IF analysis of C6 cells using GTX01528 SP1 antibody [GT1220].
Blue : DAPI
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GTX01528 IHC-P Image
IHC-P analysis of mouse kidney tissue section using GTX01528 SP1 antibody [GT1220].
Dilution : 1:100
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GTX01528 ICC/IF Image
ICC/IF analysis of NIH-3T3 cells using GTX01528 SP1 antibody [GT1220].
Blue : DAPI
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GTX01528 WB Image
WB analysis of various samples using GTX01528 SP1 antibody [GT1220].
Dilution : 1:1000
Loading : 25 μg
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文献引用
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产品评论
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![Anti-SP1 antibody [GT1220] used in Western Blot (WB). GTX01528](/upload/website/prouct_img/small/GTX01528/GTX01528_4000000128_20200417_WB_shRNA_watermark_w_23053121_180.webp "Anti-SP1 antibody [GT1220] used in Western Blot (WB). GTX01528")
![Anti-SP1 antibody [GT1220] used in Western Blot (WB). GTX01528](/upload/website/prouct_img/small/GTX01528/GTX01528_4000000128_20200410_WB_w_23053121_101.webp "Anti-SP1 antibody [GT1220] used in Western Blot (WB). GTX01528")
![Anti-SP1 antibody [GT1220] used in IHC (Paraffin sections) (IHC-P). GTX01528](/upload/website/prouct_img/small/GTX01528/GTX01528_20200508_IHC-P_1_w_23053121_526.webp "Anti-SP1 antibody [GT1220] used in IHC (Paraffin sections) (IHC-P). GTX01528")
![Anti-SP1 antibody [GT1220] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01528](/upload/website/prouct_img/small/GTX01528/GTX01528_20200508_ICCIF_1_w_23053121_684.webp "Anti-SP1 antibody [GT1220] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01528")
![Anti-SP1 antibody [GT1220] used in IHC (Paraffin sections) (IHC-P). GTX01528](/upload/website/prouct_img/small/GTX01528/GTX01528_20200508_IHC-P_w_23053121_268.webp "Anti-SP1 antibody [GT1220] used in IHC (Paraffin sections) (IHC-P). GTX01528")
![Anti-SP1 antibody [GT1220] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01528](/upload/website/prouct_img/small/GTX01528/GTX01528_20200508_ICCIF_w_23053121_871.webp "Anti-SP1 antibody [GT1220] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01528")
![Anti-SP1 antibody [GT1220] used in Western Blot (WB). GTX01528](/upload/website/prouct_img/small/GTX01528/GTX01528_20200508_WB_w_23053121_631.webp "Anti-SP1 antibody [GT1220] used in Western Blot (WB). GTX01528")
![Anti-SP1 antibody [GT1220] used in Western Blot (WB). GTX01528](/upload/website/prouct_img/normal/GTX01528/GTX01528_4000000128_20200417_WB_shRNA_watermark_w_23053121_180.webp "Anti-SP1 antibody [GT1220] used in Western Blot (WB). GTX01528")
![Anti-SP1 antibody [GT1220] used in Western Blot (WB). GTX01528](/upload/website/prouct_img/normal/GTX01528/GTX01528_4000000128_20200410_WB_w_23053121_101.webp "Anti-SP1 antibody [GT1220] used in Western Blot (WB). GTX01528")
![Anti-SP1 antibody [GT1220] used in IHC (Paraffin sections) (IHC-P). GTX01528](/upload/website/prouct_img/normal/GTX01528/GTX01528_20200508_IHC-P_1_w_23053121_526.webp "Anti-SP1 antibody [GT1220] used in IHC (Paraffin sections) (IHC-P). GTX01528")
![Anti-SP1 antibody [GT1220] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01528](/upload/website/prouct_img/normal/GTX01528/GTX01528_20200508_ICCIF_1_w_23053121_684.webp "Anti-SP1 antibody [GT1220] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01528")
![Anti-SP1 antibody [GT1220] used in IHC (Paraffin sections) (IHC-P). GTX01528](/upload/website/prouct_img/normal/GTX01528/GTX01528_20200508_IHC-P_w_23053121_268.webp "Anti-SP1 antibody [GT1220] used in IHC (Paraffin sections) (IHC-P). GTX01528")
![Anti-SP1 antibody [GT1220] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01528](/upload/website/prouct_img/normal/GTX01528/GTX01528_20200508_ICCIF_w_23053121_871.webp "Anti-SP1 antibody [GT1220] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX01528")
![Anti-SP1 antibody [GT1220] used in Western Blot (WB). GTX01528](/upload/website/prouct_img/normal/GTX01528/GTX01528_20200508_WB_w_23053121_631.webp "Anti-SP1 antibody [GT1220] used in Western Blot (WB). GTX01528")
