SP1抗体 [HL1396]

Name: SP1抗体 [HL1396]
Brand: GeneTex
SKU: GTX636836
Price: 4000.00 RMB

Anti-SP1 antibody [HL1396] used in Western Blot (WB). GTX636836

Anti-SP1 antibody [HL1396] used in IHC (Paraffin sections) (IHC-P). GTX636836

Anti-SP1 antibody [HL1396] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636836

目录号 GTX636836

文献引用 2

宿主	Rabbit
克隆	Monoclonal
克隆编号	HL1396
同种型	IgG
实验应用	WB, ICC/IF, IHC-P
种属反应	Human, Mouse, Rat
产品包装	100 μl (¥4000), 25 μl (¥1700)

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说明书
文献引用

View product citations for antibody GTX636836 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:1000-1:10000
ICC/IF	Assay dependent
IHC-P	Assay dependent

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

81 kDa. ( Note )

产品说明

This antibody is specific for human SP1 protein, and it does not cross react with human SP2, SP3, and SP4 protein.

预测种属反应

Bovine, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human SP1. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

Sp1 transcription factor

细胞定位

Nucleus,Cytoplasm

功能与背景

The protein encoded by this gene is a zinc finger transcription factor that binds to GC-rich motifs of many promoters. The encoded protein is involved in many cellular processes, including cell differentiation, cell growth, apoptosis, immune responses, response to DNA damage, and chromatin remodeling. Post-translational modifications such as phosphorylation, acetylation, glycosylation, and proteolytic processing significantly affect the activity of this protein, which can be an activator or a repressor. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2014]

数据库

Gene ID: 6667 SP1
UniProt: P08047 SP1

研究领域

產品图片

GTX636836 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody [HL1396] (GTX636836) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX636836 IHC-P Image

SP1 antibody [HL1396] detects SP1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse intestine.
SP1 stained by SP1 antibody [HL1396] (GTX636836) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX636836 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody [HL1396] (GTX636836) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX636836 IHC-P Image

SP1 antibody [HL1396] detects SP1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissue.
SP1 stained by SP1 antibody [HL1396] (GTX636836) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX636836 IHC-P Image

SP1 antibody [HL1396] detects SP1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissue.
SP1 stained by SP1 antibody [HL1396] (GTX636836) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX636836 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SP1 antibody [HL1396] (GTX636836) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX636836 IHC-P Image

SP1 antibody [HL1396] detects SP1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
SP1 stained by SP1 antibody [HL1396] (GTX636836) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX636836 IHC-P Image

GTX636836 ICC/IF Image

SP1 antibody [HL1396] detects SP1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: SP1 stained by SP1 antibody [HL1396] (GTX636836) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

文献引用

Application Reference

Fosso E et al. Antioxidants (Basel) 2023; 12 (8) Quercetin's Dual Mode of Action to Counteract the Sp1-miR-27a Axis in Colorectal Cancer Cells.

Emanuele Fosso et al. Antioxidants 2023; 12 (8) : 1547 Quercetin’s Dual Mode of Action to Counteract the Sp1-miR-27a Axis in Colorectal Cancer Cells

实验应用 : WB

种属反应 : Human

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产品评论

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Application Reference

Fosso E et al. Antioxidants (Basel) 2023; 12 (8) Quercetin's Dual Mode of Action to Counteract the Sp1-miR-27a Axis in Colorectal Cancer Cells.

Emanuele Fosso et al. Antioxidants 2023; 12 (8):1547 Quercetin’s Dual Mode of Action to Counteract the Sp1-miR-27a Axis in Colorectal Cancer Cells

实验应用 : WB

种属反应 : Human

产品包装

报价 (¥)

100 μl

¥ 4000

25 μl

¥ 1700

Qty 客制化偶联服务

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	http://www.neobioscience.com
	genetex@neobioscience.com

SP1抗体 [HL1396]

目录号 GTX636836

宿主

克隆

克隆编号

同种型

实验应用

种属反应

产品应用

应用说明

预期分子量

产品说明

预测种属反应

产品属性

形式

存储溶液

存放说明

浓度

免疫原种属

免疫原

纯化方式

偶联

注意事项

相关讯息

别名

细胞定位

功能与背景

数据库

研究领域

產品图片

GTX636836 WB Image

GTX636836 IHC-P Image

GTX636836 WB Image

GTX636836 IHC-P Image

GTX636836 IHC-P Image

GTX636836 WB Image

GTX636836 IHC-P Image

GTX636836 IHC-P Image

GTX636836 ICC/IF Image

文献引用

Application Reference

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