产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| Assay dependent |
| Assay dependent |
| 1:20-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fragmemt of human SPG7.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
SPG7 matrix AAA peptidase subunit, paraplegin , CAR , CMAR , PGN , SPG5C
细胞定位
Mitochondrion inner membrane
功能与背景
This gene encodes a mitochondrial metalloprotease protein that is a member of the AAA family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. Mutations in this gene cause autosomal recessive spastic paraplegia 7. Two transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Mar 2014]
数据库
研究领域
產品图片
![Anti-SPG7 antibody [HL2236] used in IHC (Paraffin sections) (IHC-P). GTX638281](/templates/default/js/lightbox/images/loading.gif "Anti-SPG7 antibody [HL2236] used in IHC (Paraffin sections) (IHC-P). GTX638281")
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GTX638281 IHC-P Image
SPG7 antibody [HL2236] detects SPG7 protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse tissues.SPG7 stained by SPG7 antibody [HL2236] (GTX638281) diluted at 1:20.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638281 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SPG7 antibody [HL2236] (GTX638281) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638281 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SPG7 antibody [HL2236] (GTX638281) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX638281 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SPG7 antibody [HL2236] (GTX638281) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638281 ICC/IF Image
SPG7 antibody [HL2236] detects SPG7 protein at mitochondria by immunofluorescent analysis.Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.Green: SPG7 stained by SPG7 antibody [HL2236] (GTX638281) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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文献引用
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产品评论
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![Anti-SPG7 antibody [HL2236] used in IHC (Paraffin sections) (IHC-P). GTX638281](/upload/website/prouct_img/small/GTX638281/GTX638281_T-44956_20230325_IHC-P_multiple_M_23032819_694.webp "Anti-SPG7 antibody [HL2236] used in IHC (Paraffin sections) (IHC-P). GTX638281")
![Anti-SPG7 antibody [HL2236] used in Western Blot (WB). GTX638281](/upload/website/prouct_img/small/GTX638281/GTX638281_T-44956_20230519_WB_M_23053001_287.webp "Anti-SPG7 antibody [HL2236] used in Western Blot (WB). GTX638281")
![Anti-SPG7 antibody [HL2236] used in Western Blot (WB). GTX638281](/upload/website/prouct_img/small/GTX638281/GTX638281_T-44956_20230519_WB_TPM_watermark_23053001_386.webp "Anti-SPG7 antibody [HL2236] used in Western Blot (WB). GTX638281")
![Anti-SPG7 antibody [HL2236] used in Western Blot (WB). GTX638281](/upload/website/prouct_img/small/GTX638281/GTX638281_45040_20230519_WB_R_23053001_153.webp "Anti-SPG7 antibody [HL2236] used in Western Blot (WB). GTX638281")
![Anti-SPG7 antibody [HL2236] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638281](/upload/website/prouct_img/small/GTX638281/GTX638281_T-44956_20230630_ICC_IF_23071123_776.webp "Anti-SPG7 antibody [HL2236] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638281")
![Anti-SPG7 antibody [HL2236] used in IHC (Paraffin sections) (IHC-P). GTX638281](/upload/website/prouct_img/normal/GTX638281/GTX638281_T-44956_20230325_IHC-P_multiple_M_23032819_694.webp "Anti-SPG7 antibody [HL2236] used in IHC (Paraffin sections) (IHC-P). GTX638281")
![Anti-SPG7 antibody [HL2236] used in Western Blot (WB). GTX638281](/upload/website/prouct_img/normal/GTX638281/GTX638281_T-44956_20230519_WB_M_23053001_287.webp "Anti-SPG7 antibody [HL2236] used in Western Blot (WB). GTX638281")
![Anti-SPG7 antibody [HL2236] used in Western Blot (WB). GTX638281](/upload/website/prouct_img/normal/GTX638281/GTX638281_T-44956_20230519_WB_TPM_watermark_23053001_386.webp "Anti-SPG7 antibody [HL2236] used in Western Blot (WB). GTX638281")
![Anti-SPG7 antibody [HL2236] used in Western Blot (WB). GTX638281](/upload/website/prouct_img/normal/GTX638281/GTX638281_45040_20230519_WB_R_23053001_153.webp "Anti-SPG7 antibody [HL2236] used in Western Blot (WB). GTX638281")
![Anti-SPG7 antibody [HL2236] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638281](/upload/website/prouct_img/normal/GTX638281/GTX638281_T-44956_20230630_ICC_IF_23071123_776.webp "Anti-SPG7 antibody [HL2236] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638281")
