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SQSTM1 / P62抗体 [GT1193]

Recombinant antibody KO/KD Orthogonal
Anti-SQSTM1 / P62 antibody [GT1193] used in Western Blot (WB). GTX00955
Anti-SQSTM1 / P62 antibody [GT1193] used in Western Blot (WB). GTX00955
Anti-SQSTM1 / P62 antibody [GT1193] used in IHC (Paraffin sections) (IHC-P). GTX00955
Anti-SQSTM1 / P62 antibody [GT1193] used in Western Blot (WB). GTX00955
Anti-SQSTM1 / P62 antibody [GT1193] used in Immunoprecipitation (IP). GTX00955
Anti-SQSTM1 / P62 antibody [GT1193] used in IHC (Paraffin sections) (IHC-P). GTX00955
Anti-SQSTM1 / P62 antibody [GT1193] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00955
Anti-SQSTM1 / P62 antibody [GT1193] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00955

目录号 GTX00955

文献引用 1

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 GT1193

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IP

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥5000)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
IP 1:50 - 1:200
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

48 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS, 50% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

Batch dependent (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

A synthesized peptide derived from human SQSTM1 / p62.

纯化方式


Purified by affinity chromatography

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

sequestosome 1 , A170 , FTDALS3 , OSIL , PDB3 , SQSTM1 , ZIP3 , p60 , p62 , p62B

细胞定位

Cytoplasm,Cytosol,Late endosome,Lysosome,Cytoplasmic vesicle,Autophagosome,Nucleus,Endoplasmic reticulum,PML body,Myofibril,Sarcomere

功能与背景

This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq, Mar 2009]

数据库

研究领域

產品图片

Anti-SQSTM1 / P62 antibody [GT1193] used in Western Blot (WB). GTX00955

GTX00955 WB Image

Orthogonal

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [GT1193] (GTX00955) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SQSTM1 / P62 antibody [GT1193] used in Western Blot (WB). GTX00955

GTX00955 WB Image

KO/KD

WB analysis of wild-type (WT) and SQSTM1 / p62 knockout (KO) 293T cell extracts using GTX00955 SQSTM1 / P62 antibody [GT1193].
Dilution : 1:1000
Laoding : 25μg

Anti-SQSTM1 / P62 antibody [GT1193] used in IHC (Paraffin sections) (IHC-P). GTX00955

GTX00955 IHC-P Image

IHC-P analysis of human lung cancer tissue section using GTX00955 SQSTM1 / P62 antibody [GT1193].
Dilution : 1:100

Anti-SQSTM1 / P62 antibody [GT1193] used in Western Blot (WB). GTX00955

GTX00955 WB Image

WB analysis of various samples using GTX00955 SQSTM1 / P62 antibody [GT1193].
Dilution : 1:1000
Loading : 25μg

Anti-SQSTM1 / P62 antibody [GT1193] used in Immunoprecipitation (IP). GTX00955

GTX00955 IP Image

IP analysis of HeLa cell lysate using GTX00955 SQSTM1 / P62 antibody [GT1193].
Antibody amount : 3μg / 300μg lysate
Dilution : 1:1000

Anti-SQSTM1 / P62 antibody [GT1193] used in IHC (Paraffin sections) (IHC-P). GTX00955

GTX00955 IHC-P Image

IHC-P analysis of Rat spleen tissue section using GTX00955 SQSTM1 / P62 antibody [GT1193].
Dilution : 1:100

Anti-SQSTM1 / P62 antibody [GT1193] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00955

GTX00955 ICC/IF Image

ICC/IF analysis of PFA-fixed L929 cells using GTX00955 SQSTM1 / P62 antibody [GT1193].
Orange : Primary antibody
Blue : DAPI
Dilution : 1:100

Anti-SQSTM1 / P62 antibody [GT1193] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00955

GTX00955 ICC/IF Image

ICC/IF analysis of PFA-fixed HeLa cells using GTX00955 SQSTM1 / P62 antibody [GT1193].
Orange : Primary antibody
Blue : DAPI
Dilution : 1:100

文献引用

Application Reference

Zhewen Zhang et al. Curr Issues Mol Biol 2022; 44 (6) : 2759-2771 Parkin, as a Regulator, Participates in Arsenic Trioxide-Triggered Mitophagy in HeLa Cells.
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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
产品包装 报价 (¥)
¥ 5000
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