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SQSTM1 / P62抗体 [HL1113]

Recombinant antibody Comparison Orthogonal
Anti-SQSTM1 / P62 antibody [HL1113] used in Western Blot (WB). GTX636328
Anti-SQSTM1 / P62 antibody [HL1113] used in Western Blot (WB). GTX636328
Anti-SQSTM1 / P62 antibody [HL1113] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636328
Anti-SQSTM1 / P62 antibody [HL1113] used in IHC (Paraffin sections) (IHC-P). GTX636328
Anti-SQSTM1 / P62 antibody [HL1113] used in Western Blot (WB). GTX636328
Anti-SQSTM1 / P62 antibody [HL1113] used in Western Blot (WB). GTX636328
Anti-SQSTM1 / P62 antibody [HL1113] used in Western Blot (WB). GTX636328

目录号 GTX636328

文献引用 1

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL1113

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
以下为常规应用缩写的中文注解
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

Observed MW

62 kDa.

预测种属反应

Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

保存剂

No preservative

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human SQSTM1 / P62. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

RRID

AB_2909970

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

sequestosome 1 , A170 , DMRV , FTDALS3 , NADGP , OSIL , PDB3 , ZIP3 , p60 , p62 , p62B

细胞定位

Cytoplasm, cytosol,Lysosome,Nucleus,Endoplasmic reticulum

功能与背景

This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq, Mar 2009]

数据库

研究领域

產品图片

Anti-SQSTM1 / P62 antibody [HL1113] used in Western Blot (WB). GTX636328

GTX636328 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:10000 and competitor's antibody (# Highly Cited Antibody) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-SQSTM1 / P62 antibody [HL1113] used in Western Blot (WB). GTX636328

GTX636328 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:30000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SQSTM1 / P62 antibody [HL1113] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636328

GTX636328 ICC/IF Image

Orthogonal

SQSTM1 / P62 antibody [HL1113] detects SQSTM1 / P62 protein at autophagosome by immunofluorescent analysis.
Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min.
Green: SQSTM1 / P62 stained by SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-SQSTM1 / P62 antibody [HL1113] used in IHC (Paraffin sections) (IHC-P). GTX636328

GTX636328 IHC-P Image

SQSTM1 / P62 antibody [HL1113] detects SQSTM1 / P62 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
SQSTM1 / P62 stained by SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-SQSTM1 / P62 antibody [HL1113] used in Western Blot (WB). GTX636328

GTX636328 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SQSTM1 / P62 antibody [HL1113] used in Western Blot (WB). GTX636328

GTX636328 WB Image

Orthogonal

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-SQSTM1 / P62 antibody [HL1113] used in Western Blot (WB). GTX636328

GTX636328 WB Image

Orthogonal

Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 antibody [HL1113] (GTX636328) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

文献引用

Application Reference

Wang Y et al. Front Endocrinol (Lausanne) 2022; 13 : 1051374 Calcitriol-enhanced autophagy in gingival epithelium attenuates periodontal inflammation in rats with type 2 diabetes mellitus.
实验应用 : WB
种属反应 : Rat
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