Untreated (–) and treated (+) NIH-3T3 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 (phospho Ser403) antibody (GTX128171) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 / P62 (phospho Ser403) antibody (GTX128171) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SQSTM1 / P62 (phospho Ser403) antibody detects SQSTM1 / P62 (phospho Ser403) protein at autophagosome  by immunofluorescent analysis.
Sample: Mock and treated HepG2 cells were fixed in ice-cold MeOH for 5 min.
Green: SQSTM1 / P62 (phospho Ser403) stained by SQSTM1 / P62 (phospho Ser403) antibody (GTX128171) diluted at 1:1000.
Red: alpha Tubulin, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Blue: Hoechst 33342 staining.

HepG2 cells were untreated or treated with 3μM thapsigargin for 12 and 24 hrs. Whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with SQSTM1 (phospho Ser403) antibody (GTX128171) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SQSTM1 (phospho Ser403) antibody detects SQSTM1 (phospho Ser403) protein by western blot analysis.
A. 30 μg Huh7 whole cell lysate/extract (untreated)
B. 30 μg Huh7 whole cell lysate/extract (5 μM MG132)
C. 30 μg Huh7 whole cell lysate/extract (untreated)
D. 30 μg Huh7 whole cell lysate/extract (10 μM MG132)
SQSTM1 (phospho Ser403) antibody (GTX128171) dilution: 1:500
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

SQSTM1 (phospho Ser403) antibody detects SQSTM1 (phospho Ser403) protein at nucleus on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
SQSTM1 (phospho Ser403) antibody (GTX128171) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SQSTM1 (phospho Ser403) antibody (GTX128171) detects SQSTM1 (phospho Ser403) protein by flow cytometry analysis.
Sample: HeLa cell fixed in 4% paraformaldehyde at 4oC for 5 min.
Brown: Unlabelled sample was also used as a control.
Blue: SQSTM1 (phospho Ser403) antibody (GTX128171) dilution: 1:100.
Acquisition of >20,000 events were collected using Argon ion laser (488nm) and 525/30 bandpass filter.

SQSTM1 (phospho Ser403) antibody detects SQSTM1 (phospho Ser403) protein at cytosol on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human colon carcinoma.
SQSTM1 (phospho Ser403) antibody (GTX128171) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

The data was published in the journal Physiol Rep in 2017. PMID: 29263116

The data was published in the journal Physiol Rep in 2017. PMID: 29263116

The data was published in the 2019 in Cell Death Dis. PMID: 31320609