产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:25000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
This antibody is specific for human STAM protein, and it does not cross react with human STAM2 protein.
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fragment of human STAM
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
signal transducing adaptor molecule , STAM-1 , STAM1
细胞定位
Cytoplasm,Early endosome membrane
功能与背景
This gene encodes a member of the signal-transducing adaptor molecule family. These proteins mediate downstream signaling of cytokine receptors and also play a role in ER to Golgi trafficking by interacting with the coat protein II complex. The encoded protein also associates with hepatocyte growth factor-regulated substrate to form the endosomal sorting complex required for transport-0 (ESCRT-0), which sorts ubiquitinated membrane proteins to the ESCRT-1 complex for lysosomal degradation. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Feb 2011]
数据库
研究领域
產品图片
![Anti-STAM antibody [HL1637] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637130](/templates/default/js/lightbox/images/loading.gif "Anti-STAM antibody [HL1637] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637130")
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GTX637130 ICC/IF Image
STAM antibody [HL1637] detects STAM protein at endosome by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: STAM stained by STAM antibody [HL1637] (GTX637130) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX637130 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with STAM antibody [HL1637] (GTX637130) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637130 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (10 | 10 | 20 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with STAM antibody [HL1637] (GTX637130) diluted at 1:25000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637130 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with STAM antibody [HL1637] (GTX637130) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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文献引用
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产品评论
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![Anti-STAM antibody [HL1637] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637130](/upload/website/prouct_img/small/GTX637130/GTX637130_T-44739_20220826_ICC_IF_23112021_328.webp "Anti-STAM antibody [HL1637] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637130")
![Anti-STAM antibody [HL1637] used in Western Blot (WB). GTX637130](/upload/website/prouct_img/small/GTX637130/GTX637130_T-44739_20231110_WB_H_M_23112021_178.webp "Anti-STAM antibody [HL1637] used in Western Blot (WB). GTX637130")
![Anti-STAM antibody [HL1637] used in Western Blot (WB). GTX637130](/upload/website/prouct_img/small/GTX637130/GTX637130_44788_20231110_WB_multiple_B_23111422_365.webp "Anti-STAM antibody [HL1637] used in Western Blot (WB). GTX637130")
![Anti-STAM antibody [HL1637] used in Western Blot (WB). GTX637130](/upload/website/prouct_img/small/GTX637130/GTX637130_44788_20231110_WB_TPM_watermark_23111422_518.webp "Anti-STAM antibody [HL1637] used in Western Blot (WB). GTX637130")
![Anti-STAM antibody [HL1637] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637130](/upload/website/prouct_img/normal/GTX637130/GTX637130_T-44739_20220826_ICC_IF_23112021_328.webp "Anti-STAM antibody [HL1637] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637130")
![Anti-STAM antibody [HL1637] used in Western Blot (WB). GTX637130](/upload/website/prouct_img/normal/GTX637130/GTX637130_T-44739_20231110_WB_H_M_23112021_178.webp "Anti-STAM antibody [HL1637] used in Western Blot (WB). GTX637130")
![Anti-STAM antibody [HL1637] used in Western Blot (WB). GTX637130](/upload/website/prouct_img/normal/GTX637130/GTX637130_44788_20231110_WB_multiple_B_23111422_365.webp "Anti-STAM antibody [HL1637] used in Western Blot (WB). GTX637130")
![Anti-STAM antibody [HL1637] used in Western Blot (WB). GTX637130](/upload/website/prouct_img/normal/GTX637130/GTX637130_44788_20231110_WB_TPM_watermark_23111422_518.webp "Anti-STAM antibody [HL1637] used in Western Blot (WB). GTX637130")
