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STAT3抗体 [C3], C-term

KO/KD Comparison Orthogonal
Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616
Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616
Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616
Anti-STAT3 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104616
Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616
Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616
Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616
Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616
Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616
Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616
Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616
Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616
Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616
Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616
Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616

目录号 GTX104616

文献引用 30
4

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse, Rat, Plant
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 STAT3 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

88 kDa. ( Note )

阳性对照

Human ESC , OC3

预测种属反应

Bovine, Chicken, Pig(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.48 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human STAT3. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1952032

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

signal transducer and activator of transcription 3 , ADMIO , ADMIO1 , APRF , HIES

细胞定位

Cytoplasm , Nucleus

功能与背景

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Three alternatively spliced transcript variants encoding distinct isoforms have been described. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616

GTX104616 WB Image

KO/KD

Non-transfected (–) and transfected (+) OC3 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT3 antibody [C3], C-term (GTX104616) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616

GTX104616 WB Image

KO/KD

Wild-type (WT) and STAT3 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT3 antibody [C3], C-term (GTX104616) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616

GTX104616 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with STAT3 antibody [C3], C-term (GTX104616) diluted at 1:1000 and competitor's antibody diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-STAT3 antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX104616

GTX104616 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using STAT3(GTX104616) antibody at 1:200 dilution.

Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616

GTX104616 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT3 antibody [C3], C-term (GTX104616) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616

GTX104616 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT3 antibody [C3], C-term (GTX104616) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616

GTX104616 WB Image

Sample (20 μg of whole cell lysate)
A: human ESC
7.5% SDS PAGE
GTX104616 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616

GTX104616 WB Image

Orthogonal

The data was published in the journal Cancer Cell Int in 2020.PMID: 31920463

Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616

GTX104616 WB Image

Orthogonal

The data was published in the journal Cancer Cell Int in 2020.PMID: 31920463

Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616

GTX104616 WB Image

Orthogonal

The data was published in the 2019 in J Exp Clin Cancer Res. PMID: 30961670

Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616

GTX104616 WB Image

The data was published in the journal Int J Mol Sci in 2018. PMID: 29794990

Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616

GTX104616 WB Image

The data was published in the journal Int J Mol Sci in 2018. PMID: 29794990

Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616

GTX104616 WB Image

The data was published in the 2022 in Cell Death Dis. PMID: 35296653

Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616

GTX104616 WB Image

The data was published in the 2022 in Cell Death Dis. PMID: 35296653

Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616

GTX104616 WB Image

The data was published in the 2020 in PLoS One. PMID: 33382817

文献引用

Application Reference

Xu W et al. Immune Netw 2023; 23 (6) : e46 IL-17 Imbalance Promotes the Pyroptosis in Immune-Mediated Liver Injury Through STAT3-IFI16 Axis.
W Xu et al. Immune Netw 2023; 23 (6) : e46 IL-17 Imbalance Promotes the Pyroptosis in Immune-Mediated Liver Injury Through STAT3-IFI16 Axis
实验应用 : WB
BY Wang et al. Am J Cancer Res 2023; 13 (10) : 4721-4733 Inhibition of Na+/H+ exchanger (NHE) 7 by 5-(N-ethyl-N-isopropyl)-Amiloride displays anti-cancer activity in non-small cell lung cancer by disrupting cancer stem cell activity and downregulating PD-L1 expression
Liao CP et al. Biomedicines 2023; 11 (10) Methoxyhispolon Methyl Ether, a Hispolon Analog, Thwarts the SRC/STAT3/BCL-2 Axis to Provoke Human Triple-Negative Breast Cancer Cell Apoptosis In Vitro.
Hsieh YC et al. Biomedicines 2023; 11 (9) Blockade of the SRC/STAT3/BCL-2 Signaling Axis Sustains the Cytotoxicity in Human Colorectal Cancer Cell Lines Induced by Dehydroxyhispolon Methyl Ether.
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产品评论

STAT3 antibody [C3], C-term 目录号 GTX104616

Rating
( Average 4.3 based on 4 users reviews)
实验应用
Western Blot(WB)
( Average 4.3 based on 4 users reviews)
Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616
Date : Anonymous submitted on 01-Jun-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : CH27 cells
Amount used : 20μg
Blocking : Blocking buffer, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 18Hr
Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616
Date : Anonymous submitted on 28-Oct-2020
Score :
Application Tested : WB
Sample Species : Hu
Sample : T24 cells
Amount used : 30 μg
Blocking : 5% non-fat milk in PBST, 37°C, 1.5Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616
Date : Anonymous submitted on 04-Oct-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : LAMA-84 cells
Amount used : 20μg
Blocking : 5% BSA in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Anti-STAT3 antibody [C3], C-term used in Western Blot (WB). GTX104616
Date : Anonymous submitted on 14-Aug-2014
Score :
Application Tested : WB
Sample Species : Hu
Sample : HepG2 cells
Amount used : 30μg
Blocking : TBST with 0.5% BSA, 4°C, 18Hr
Primary Antibody : 1:1000, 24°C, 2Hr
我要评论产品
Application Reference
Xu W et al. Immune Netw 2023; 23 (6):e46 IL-17 Imbalance Promotes the Pyroptosis in Immune-Mediated Liver Injury Through STAT3-IFI16 Axis.
W Xu et al. Immune Netw 2023; 23 (6):e46 IL-17 Imbalance Promotes the Pyroptosis in Immune-Mediated Liver Injury Through STAT3-IFI16 Axis
实验应用 : WB
BY Wang et al. Am J Cancer Res 2023; 13 (10):4721-4733 Inhibition of Na+/H+ exchanger (NHE) 7 by 5-(N-ethyl-N-isopropyl)-Amiloride displays anti-cancer activity in non-small cell lung cancer by disrupting cancer stem cell activity and downregulating PD-L1 expression
Liao CP et al. Biomedicines 2023; 11 (10) Methoxyhispolon Methyl Ether, a Hispolon Analog, Thwarts the SRC/STAT3/BCL-2 Axis to Provoke Human Triple-Negative Breast Cancer Cell Apoptosis In Vitro.
Hsieh YC et al. Biomedicines 2023; 11 (9) Blockade of the SRC/STAT3/BCL-2 Signaling Axis Sustains the Cytotoxicity in Human Colorectal Cancer Cell Lines Induced by Dehydroxyhispolon Methyl Ether.
Hsu CY et al. Int J Mol Sci 2023; 24 (14) Sertindole, an Antipsychotic Drug, Curbs the STAT3/BCL-xL Axis to Elicit Human Bladder Cancer Cell Apoptosis In Vitro.
Cardile A et al. Int J Mol Sci 2023; 24 (2) Hyperforin Elicits Cytostatic/Cytotoxic Activity in Human Melanoma Cell Lines, Inhibiting Pro-Survival NF-κB, STAT3, AP1 Transcription Factors and the Expression of Functional Proteins Involved in Mitochondrial and Cytosolic Metabolism.
Kuo MY et al. Int J Mol Sci 2022; 24 (1) Hispolon Methyl Ether, a Hispolon Analog, Suppresses the SRC/STAT3/Survivin Signaling Axis to Induce Cytotoxicity in Human Urinary Bladder Transitional Carcinoma Cell Lines.
实验应用 : WB
种属反应 : Human
Mang Y et al. Ann Transl Med 2022; 10 (19):1066 Characteristics of pre-sensitization-related acute antibody-mediated rejection in a rat model of orthotopic liver transplantation.
实验应用 : WB
种属反应 : Rat
Vidhya Tangeda et al. Cell Death Dis 2022; 13 (3):241
实验应用 : WB
种属反应 : Human
Lecheng Zhang et al. Cell Cycle 2021; 20 (11):1052-1066 Knockdown of hsa_circ_0134111 alleviates the symptom of osteoarthritis via sponging microRNA-224-5p.
Raineri A et al. Molecules. 2021; 26 (16):5027 3,5-Dicaffeoylquinic Acid Lowers 3T3-L1 Mitotic Clonal Expansion and Adipocyte Differentiation by Enhancing Heme Oxygenase-1 Expression.
实验应用 : WB
种属反应 : Mouse
Teo WH et al. PLoS One 2020; 15 (12):e0244791 Ganoderma microsporum immunomodulatory protein, GMI, promotes C2C12 myoblast differentiation in vitro via upregulation of Tid1 and STAT3 acetylation.
实验应用 : WB
种属反应 : Mouse
Ogawa T et al. Am J Pathol 2020; 190 (3):577-585 Nuclear Factor Erythroid 2-Related Factor 2 (Nrf2) Regulates Epidermal Keratinization under Psoriatic Skin Inflammation.
实验应用 : IHC-P
种属反应 : Mouse
Wang YY et al. ACS Omega 2020; 5 (12):7018-7024 Orabase-Formulated Benzalkonium Chloride Effectively Suppressed Oral Potentially Malignant Disorder In Vitro and In Vivo.
实验应用 : WB
种属反应 : Human
Yang Y et al. Cancer Cell Int 2020; 20:9 Silencing of AURKA augments the antitumor efficacy of the AURKA inhibitor MLN8237 on neuroblastoma cells.
实验应用 : WB
种属反应 : Human
Chen YL et al. Cancers (Basel) 2019; 12 (1) ERK Activation Modulates Cancer Stemness and Motility of a Novel Mouse Oral Squamous Cell Carcinoma Cell Line.
Fan JJ et al. Antioxidants (Basel) 2019; 8 (11) Dietary Flavonoids Luteolin and Quercetin Inhibit Migration and Invasion of Squamous Carcinoma through Reduction of Src/Stat3/S100A7 Signaling.
实验应用 : WB
种属反应 : Human
Qin F et al. J Enzyme Inhib Med Chem 2019; 34 (1):909-926 Design, synthesis and molecular mechanisms of novel dual inhibitors of heat shock protein 90/phosphoinositide 3-kinase alpha (Hsp90/PI3Kα) against cutaneous melanoma.
实验应用 : WB
种属反应 : Human
Shen M et al. J Exp Clin Cancer Res 2019; 38 (1):149
实验应用 : WB
种属反应 : Human
Chen PC et al. Int J Mol Sci 2018; 19 (6) anti-metastatic effects of antrodan with and without * on lewis lung carcinomas in a mouse xenograft model.
实验应用 : WB
种属反应 : Mouse
Lin C.I. et al. Int. J. Biol. Sci. 2018; 14 (3):253-265 Instillation of particulate matter 2.5 induced acute lung injury and attenuated the injury recovery in ACE2 knockout mice.
实验应用 : WB
种属反应 : Mouse
Lin JT et al. Food Funct. 2017; 8 (3):1245-1253 Molecular mechanisms of the effects of the ethanolic extract of Muntingia calabura Linn. fruit on lipopolysaccharide-induced pro-inflammatory mediators in macrophages.
实验应用 : WB
种属反应 : Plant
Li X et al. Biomed Pharmacother 2017; 96:434-442 Targeting of cell cycle and let-7a/STAT3 pathway by niclosamide inhibits proliferation, migration and invasion in oral squamous cell carcinoma cells.
实验应用 : WB
种属反应 : Human
Aalinkeel R et al. J Neuroimmune Pharmacol 2016; (Epub) Galectin-1 Reduces Neuroinflammation via Modulation of Nitric Oxide-Arginase Signaling in HIV-1 Transfected Microglia: a Gold Nanoparticle-Galectin-1 "Nanoplex" a Possible Neurotherapeutic?
实验应用 : ICC/IF
种属反应 : Human
Chiu WC et al. PLoS One 2016; 11 (11):e0166453 The Synthetic β-Nitrostyrene Derivative CYT-Rx20 Inhibits Esophageal Tumor Growth and Metastasis via PI3K/AKT and STAT3 Pathways.
实验应用 : WB
种属反应 : Human
Huang JY et al. Sci Rep 2016; 6:34740 MicroRNA miR-204 and miR-1236 inhibit hepatitis B virus replication via two different mechanisms.
实验应用 : WB
种属反应 : Human
Hung AC et al. Clin Cancer Res 2016; (Epub) Extracellular Visfatin-Promoted Malignant Behavior in Breast Cancer Is Mediated Through c-Abl and STAT3 Activation.
实验应用 : WB
种属反应 : Human
Chen YT et al. Oncotarget 2015; 6 (15) ptprd silencing by dna hypermethylation decreases * receptor signaling and leads to type 2 diabetes.
实验应用 : WB
种属反应 : Human
Wang SH et al. Gastroenterology 2012; 142 (4):989-998.e4 Estrogen receptor α represses transcription of HBV genes via interaction with hepatocyte nuclear factor 4α.
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产品包装 报价 (¥)
¥ 4000
¥ 1700
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