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STAT3抗体 [HL1134]

Recombinant antibody KO/KD Comparison
Anti-STAT3 antibody [HL1134] used in Western Blot (WB). GTX636400
Anti-STAT3 antibody [HL1134] used in Western Blot (WB). GTX636400
Anti-STAT3 antibody [HL1134] used in IHC (Paraffin sections) (IHC-P). GTX636400
Anti-STAT3 antibody [HL1134] used in Western Blot (WB). GTX636400
Anti-STAT3 antibody [HL1134] used in Western Blot (WB). GTX636400
Anti-STAT3 antibody [HL1134] used in Western Blot (WB). GTX636400

目录号 GTX636400

文献引用 2
1

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL1134

同种型

 IgG

实验应用

 WB, IHC-P

种属反应

 Human, Mouse, Rat, Cat, Dog
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 STAT3 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
IHC-P Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

88 kDa. ( Note )

预测种属反应

Zebrafish, Bovine, Chicken, Pig, Japanese Medaka(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the N-terminus region of human STAT3. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

RRID

AB_2909976

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

signal transducer and activator of transcription 3 , ADMIO , ADMIO1 , APRF , HIES

细胞定位

Cytoplasm,Nucleus

功能与背景

The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated through phosphorylation in response to various cytokines and growth factors including IFNs, EGF, IL5, IL6, HGF, LIF and BMP2. This protein mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis. The small GTPase Rac1 has been shown to bind and regulate the activity of this protein. PIAS3 protein is a specific inhibitor of this protein. Mutations in this gene are associated with infantile-onset multisystem autoimmune disease and hyper-immunoglobulin E syndrome. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2015]

数据库

研究领域

產品图片

Anti-STAT3 antibody [HL1134] used in Western Blot (WB). GTX636400

GTX636400 WB Image

KO/KD

Wild-type (WT) and STAT3 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT3 antibody [HL1134] (GTX636400) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-STAT3 antibody [HL1134] used in Western Blot (WB). GTX636400

GTX636400 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with STAT3 antibody [HL1134] (GTX636400) diluted at 1:1000 and competitor's antibody (#Highly competitor antibody) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-STAT3 antibody [HL1134] used in IHC (Paraffin sections) (IHC-P). GTX636400

GTX636400 IHC-P Image

STAT3 antibody [HL1134] detects STAT3 protein at cytoplasm and nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
STAT3 stained by STAT3 antibody [HL1134] (GTX636400) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-STAT3 antibody [HL1134] used in Western Blot (WB). GTX636400

GTX636400 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT3 antibody [HL1134] (GTX636400) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-STAT3 antibody [HL1134] used in Western Blot (WB). GTX636400

GTX636400 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT3 antibody [HL1134] (GTX636400) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-STAT3 antibody [HL1134] used in Western Blot (WB). GTX636400

GTX636400 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT3 antibody [HL1134] (GTX636400) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

文献引用

Application Reference

Shen Y et al. Molecules 2023; 29 (1) Transformation of Ginsenosides by Lactiplantibacillus plantarum MB11 Fermentation: Minor Ginsenosides Conversion and Enhancement of Anti-Colorectal Cancer Activity.
Yunjiao Shen et al. Authorea. 2023; Transformation of ginsenosides by Lactiplantibacillus plantarum MB11 fermentation: minor ginsenosides conversion and enhancement of anti-colorectal cancer activity
实验应用 : WB
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产品评论

STAT3 antibody [HL1134] 目录号 GTX636400

Rating
( Average 5 based on 1 users reviews)
实验应用
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-STAT3 antibody [HL1134] used in Western Blot (WB). GTX636400
Date : Anonymous submitted on 01-Jun-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : CH27 cells
Amount used : 20μg
Blocking : Blocking buffer, 25°C, 1Hr
Primary Antibody : 1:1000, °C, 18Hr
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产品包装 报价 (¥)
¥ 4000
¥ 1700
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