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STAT5A抗体 [C2C3], C-term

Anti-STAT5A antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX113351
Anti-STAT5A antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113351
Anti-STAT5A antibody [C2C3], C-term used in Western Blot (WB). GTX113351
Anti-STAT5A antibody [C2C3], C-term used in Western Blot (WB). GTX113351
Anti-STAT5A antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX113351
Anti-STAT5A antibody [C2C3], C-term used in Western Blot (WB). GTX113351

目录号 GTX113351

文献引用 3
1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 STAT5A 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:10000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

91 kDa. ( Note )

阳性对照

293T , A431 , HeLa , HepG2 , OECM-1 , SCC-9

预测种属反应

Rat, Sheep, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

0.1M Tris, 0.1M Glycine, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the C-terminus region of human STAT5A. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_11176176

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

signal transducer and activator of transcription 5A , MGF , STAT5

细胞定位

Cytoplasm , Nucleus

功能与背景

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-STAT5A antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX113351

GTX113351 IHC-P Image

STAT5A antibody [C2C3], C-term detects STAT5A protein at cytosol and nucleus on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
STAT5A antibody [C2C3], C-term (GTX113351) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-STAT5A antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX113351

GTX113351 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using STAT5A(GTX113351) antibody at 1:500 dilution.

Anti-STAT5A antibody [C2C3], C-term used in Western Blot (WB). GTX113351

GTX113351 WB Image

Whole cell extract (12.7 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT5A antibody [C2C3], C-term (GTX113351) diluted at 1:5000.

Anti-STAT5A antibody [C2C3], C-term used in Western Blot (WB). GTX113351

GTX113351 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: A431
C: HeLa
D: HepG2
7.5% SDS PAGE
GTX113351 diluted at 1:1000

Anti-STAT5A antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX113351

GTX113351 IHC-P Image

STAT5A antibody [C2C3], C-term detects STAT5A protein at cytosol and nucleus on mouse fore brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse fore brain.
STAT5A antibody [C2C3], C-term (GTX113351) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-STAT5A antibody [C2C3], C-term used in Western Blot (WB). GTX113351

GTX113351 WB Image

Whole cell extract (22.5 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with STAT5A antibody [C2C3], C-term (GTX113351) diluted at 1:2000.

文献引用

Application Reference

Li CJ et al. J Hepatocell Carcinoma 2023; 10 : 123-138
实验应用 : WB
种属反应 : Human
Chi M et al. Sci Rep 2016; 6 : 33132 Phosphorylation of calcium/calmodulin-stimulated protein kinase II at T286 enhances invasion and migration of human breast cancer cells.
实验应用 : WB
种属反应 : Human
Lee MC et al. J Leukoc Biol 2014; 95 (1) : 105-15 Gfi-1 is the transcriptional repressor of SOCS1 in acute myeloid leukemia cells.
实验应用 : WB
种属反应 : Human
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产品评论

STAT5A antibody [C2C3], C-term 目录号 GTX113351

Rating
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实验应用
Western Blot(WB)
( Average 4 based on 1 users reviews)
Anti-STAT5A antibody [C2C3], C-term used in Western Blot (WB). GTX113351
Date : Anonymous submitted on 05-Jul-2016
Score :
Application Tested : WB
Sample Species : Hu
Sample : HSC-3 cells
Amount used : 15μg
Blocking : 5% milk in 0.1% TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
我要评论产品
SDS
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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