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Sox2抗体

Anti-Sox2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX124477
Anti-Sox2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124477
Anti-Sox2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124477
Anti-Sox2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124477
Anti-Sox2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124477
Anti-Sox2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124477
Anti-Sox2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124477
Anti-Sox2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124477
Anti-Sox2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124477

目录号 GTX124477

文献引用 19
1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC-Fr, IHC-Wm

种属反应

 Zebrafish, Japanese Medaka, Fish
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 zebrafish Sox2 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
IHC-Fr 1:100-1:1000
IHC-Wm 1:50-1:500
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

35 kDa. ( Note )

预测种属反应

Human (100%)| Mouse (99%)| Rat (99%)| Zebrafish (92%)| Sheep (100%)| Bovine (100%)| Cat (100%)| Chicken (94%)| Pig (98%)| Japanese medaka (88%)| Xenopus tropicalis (90%)| Rhesus Monkey (100%)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.41 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原

The immunogen used to generate this antibody corresponds to zebrafish Sox2.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_11178063

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

SRY (sex determining region Y)-box 2 , cb236 , soxp , wu:fb83g04 , wu:fc14d07 , zgc:65860 , zgc:77389

细胞定位

Nucleus , Cytoplasm

功能与背景

Transcriptional activator. May function as a switch in neuronal development.

数据库

研究领域

產品图片

Anti-Sox2 antibody used in IHC (Frozen sections) (IHC-Fr). GTX124477

GTX124477 IHC-Fr Image

Immunohistochemical analysis of frozen sections of zebrafish retina, using sox2 (GTX124477) antibody at 1:1000 dilution.

Anti-Sox2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124477

GTX124477 IHC-Wm Image

Sox2 antibody detects Sox2 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Sox2 antibody (GTX124477) dilution: 1:50.
Anti-Sox2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124477

GTX124477 IHC-Wm Image

Sox2 antibody detects Sox2 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Sox2 antibody (GTX124477) dilution: 1:100.

Anti-Sox2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124477

GTX124477 IHC-Wm Image

Sox2 antibody detects Sox2 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Sox2 antibody (GTX124477) dilution: 1:100.
Anti-Sox2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124477

GTX124477 IHC-Wm Image

Sox2 antibody detects Sox2 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Sox2 antibody (GTX124477) dilution: 1:100.
Anti-Sox2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124477

GTX124477 IHC-Wm Image

Sox2 antibody detects Sox2 protein on Medaka by whole mount immunohistochemical analysis.
Sample: 7 days-post-fertilization medaka embryo.
Sox2 antibody (GTX124477) dilution: 1:100.
Anti-Sox2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124477

GTX124477 IHC-Wm Image

Sox2 antibody detects Sox2 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Sox2 antibody (GTX124477) dilution: 1:100.

Anti-Sox2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124477

GTX124477 IHC-Wm Image

The data was published in the journal Sci Rep in 2017.PMID: 28717156

Anti-Sox2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX124477

GTX124477 ICC/IF Image

The data was published in the journal iScience in 2019. PMID: 31055217

文献引用

Application Reference

Sun K et al. iScience 2023; 26 (11) : 108103 The splicing factor DHX38 enables retinal development through safeguarding genome integrity.
Brown TL et al. Elife 2023; 12 Dermal appendage-dependent patterning of zebrafish atoh1a+ Merkel cells.
Saraswathy VM et al. Cell Rep 2022; 41 (8) : 111705 Myostatin is a negative regulator of adult neurogenesis after spinal cord injury in zebrafish.
Jimenez E et al. Cell Genom 2022; 2 (9) A regulatory network of Sox and Six transcription factors initiate a cell fate transformation during hearing regeneration in adult zebrafish.
实验应用 : IHC-Wm
种属反应 : Zebrafish
Diego P?rez-Dones et al. Biomedicines 2021; 9 (9) Quantitative Approaches to Study Retinal Neurogenesis.
种属反应 : Zebrafish
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产品评论

Sox2 antibody 目录号 GTX124477

Rating
( Average 4 based on 1 users reviews)
实验应用
IHC (Whole mount)(IHC-Wm)
( Average 4 based on 1 users reviews)
Anti-Sox2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124477
Date : Anonymous submitted on 21-Jul-2021
Score :
Application Tested : IHC-Wm
Sample Species : Zfsh
Sample : Zebrafish embryo, 20 hpf
Blocking : 5% BSA, 0.5% TritonX100, 0.5% Tween20 in PBS, 25°C, 2Hr
Fixation : 4% PFA in PBS
Permeabilization /Antigen retrieval : 1% Triton in PBS
Primary Antibody : 1:200, 4°C, 16Hr
Figure Note : Sox2 antibody detects Sox2 protein (Alexa 488) in the zebrafish neural tube by whole mount immunohistochemical analysis.
我要评论产品
Application Reference
Sun K et al. iScience 2023; 26 (11):108103 The splicing factor DHX38 enables retinal development through safeguarding genome integrity.
Brown TL et al. Elife 2023; 12 Dermal appendage-dependent patterning of zebrafish atoh1a+ Merkel cells.
Saraswathy VM et al. Cell Rep 2022; 41 (8):111705 Myostatin is a negative regulator of adult neurogenesis after spinal cord injury in zebrafish.
Jimenez E et al. Cell Genom 2022; 2 (9) A regulatory network of Sox and Six transcription factors initiate a cell fate transformation during hearing regeneration in adult zebrafish.
实验应用 : IHC-Wm
种属反应 : Zebrafish
Diego P?rez-Dones et al. Biomedicines 2021; 9 (9) Quantitative Approaches to Study Retinal Neurogenesis.
种属反应 : Zebrafish
Zilova L et al. Elife 2021; 10 Fish primary embryonic pluripotent cells assemble into retinal tissue mirroring in vivo early eye development.
实验应用 : IHC-Wm
种属反应 : Japanese Medaka
Hardy K et al. J Physiol 2021; 599 (16):3913-3936 Functional development and regeneration of hair cells in the zebrafish lateral line.
种属反应 : Zebrafish
Li J et al. Nucleic Acids Res 2021; 49 (4):2027-2043 Prpf31 is essential for the survival and differentiation of retinal progenitor cells by modulating alternative splicing.
实验应用 : IHC-Wm
种属反应 : Zebrafish
Ledesma-Terr?n M et al. Development 2020; 147 (14) FGF2 modulates simultaneously the mode, the rate of division and the growth fraction in cultures of radial glia.
Nagashima M et al. J Neurosci 2020; 40 (6):1232-1247 Midkine-a Is Required for Cell Cycle Progression of M?ller Glia during Neuronal Regeneration in the Vertebrate Retina.
实验应用 : IHC-Wm
种属反应 : Zebrafish
Hideki Ando et al. iScience 2019; 15:95-108 Cereblon Control of Zebrafish Brain Size by Regulation of Neural Stem Cell Proliferation.
实验应用 : ICC/IF
种属反应 : Zebrafish
Bloomquist RF et al. Proc Natl Acad Sci U S A 2019; 116 (36):17858-17866 Developmental plasticity of epithelial stem cells in tooth and taste bud renewal.
实验应用 : IHC-P
种属反应 : Fish
Mario Ledesma-Terr?n et al. bioRxiv 2019; (Epub) Radial Glial from mammalian developing neocortex can divide symmetrically in vitro.
实验应用 : ICC/IF
Xia W et al. FEBS Lett 2019; (Epub) Mutations in TOP2B cause autosomal-dominant hereditary hearing loss via inhibition of the PI3K-Akt signalling pathway.
实验应用 : IHC-Wm
种属反应 : Zebrafish
Bayramli X et al. Sci Rep 2017; 7 (1):5572 Patterned Arrangements of Olfactory Receptor Gene Expression in Zebrafish are Established by Radial Movement of Specified Olfactory Sensory Neurons.
实验应用 : IHC-Wm
种属反应 : Zebrafish
Seleit A et al. bioRxiv 2017; (Epub) Neural stem cells induce the formation of their physical niche during organogenesis.
种属反应 : Japanese Medaka
Gorsuch RA et al. Exp Eye Res 2017; (Epub) Sox2 regulates Muller glia reprogramming and proliferation in the regenerating zebrafish retina via Lin28 and Ascl1a.
实验应用 : WB
种属反应 : Zebrafish
Bloomquist, Ryan F et al. 0 2015; Developmental plasticity of stem cells in teeth and taste bud renewal.
实验应用 : IHC-P
种属反应 : Zebrafish
Ogai K et al. Neurosci Res 2014; Function of Sox2 in ependymal cells of lesioned spinal cords in adult zebrafish.
实验应用 : IHC-Wm
种属反应 : Zebrafish
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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