产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
Assay dependent |
1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
预测种属反应
Rat, Sheep, Bovine, Pig, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human StAR. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
steroidogenic acute regulatory protein , STARD1
细胞定位
Mitochondrion
功能与背景
The protein encoded by this gene plays a key role in the acute regulation of steroid hormone synthesis by enhancing the conversion of cholesterol into pregnenolone. This protein permits the cleavage of cholesterol into pregnenolone by mediating the transport of cholesterol from the outer mitochondrial membrane to the inner mitochondrial membrane. Mutations in this gene are a cause of congenital lipoid adrenal hyperplasia (CLAH), also called lipoid CAH. A pseudogene of this gene is located on chromosome 13. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
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GTX636800 IHC-P Image
StAR antibody [HL1360] detects StAR protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded mouse adrenal gland.StAR stained by StAR antibody [HL1360] (GTX636800) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636800 IHC-P Image
StAR antibody [HL1360] detects StAR protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse kidney.StAR stained by StAR antibody [HL1360] (GTX636800) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636800 ICC/IF Image
StAR antibody [HL1360] detects StAR protein by immunofluorescent analysis.Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: StAR stained by StAR antibody [HL1360] (GTX636800) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX636800 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with StAR antibody [HL1360] (GTX636800) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX636800 IHC-P Image
StAR antibody [HL1360] detects StAR protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded cat kidney.StAR stained by StAR antibody [HL1360] (GTX636800) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636800 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with StAR antibody [HL1360] (GTX636800) diluted at 1:200000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636800 WB Image
Various tissue extracts (50 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with StAR antibody [HL1360] (GTX636800) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636800 IHC-P Image
StAR antibody [HL1360] detects StAR protein at mitochondria by immunohistochemical analysis.Sample: Paraffin-embedded dog kidney.StAR stained by StAR antibody [HL1360] (GTX636800) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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