Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Strep epitope Tag antibody [GT517] (GTX628899) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Strep epitope Tag antibody [GT517] detects Strep-recombinant protein by immunofluorescent analysis. Sample: Strep/GFP-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: Strep-recombinant protein stained by Strep epitope Tag antibody [GT517] (GTX628899) diluted at 1:500. Green: GFP is expressed in the tranfected cell. Blue: Hoechst 33342 staining.