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Strep epitope Tag抗体 [GT661]

Anti-Strep epitope Tag antibody [GT661] used in Western Blot (WB). GTX628900
Anti-Strep epitope Tag antibody [GT661] used in Immunoprecipitation (IP). GTX628900
Anti-Strep epitope Tag antibody [GT661] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628900

目录号 GTX628900

文献引用 1
1

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 GT661

同种型

 IgG2a

实验应用

 WB, ICC/IF, IP

种属反应

 Species independent
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
IP 1:100-1:500
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原

Immunogen Amino acid sequence - ASWSHPQFEKGA

纯化方式

Affinity purified by Protein G.

偶联

Unconjugated

RRID

AB_2888076

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

功能与背景

Strep epitope tag is a peptide sequence with high specificity and affinity toward Streptavidin.

研究领域

產品图片

Anti-Strep epitope Tag antibody [GT661] used in Western Blot (WB). GTX628900

GTX628900 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Strep epitope Tag antibody [GT661] (GTX628900) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Strep epitope Tag antibody [GT661] used in Immunoprecipitation (IP). GTX628900

GTX628900 IP Image

Strep epitope Tag antibody [GT661] immunoprecipitates strep tagged protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 20 μg whole cell lysate/extract of strep tagged protein expressing 293T cells B : Control with 2.5 μg of pre-immune mouse IgG C : Immunoprecipitation of strep tagged protein by 2.5 μg of Strep epitope Tag antibody [GT661] (GTX628900) 10% SDS-PAGE The immunoprecipitated strep tagged protein was detected by Strep epitope Tag antibody [GT661] (GTX628900) diluted at 1 : 3000. EasyBlot anti-mouse IgG (HRP) (GTX221667-01) was used as a secondary reagent.

Anti-Strep epitope Tag antibody [GT661] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX628900

GTX628900 ICC/IF Image

Strep epitope Tag antibody [GT661] detects Strep-recombinant protein by immunofluorescent analysis. Sample: Strep/GFP-tag transfected HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Red: Strep-recombinant protein stained by Strep epitope Tag antibody [GT661] (GTX628899) diluted at 1:500. Green: GFP is expressed in the tranfected cell. Blue: Hoechst 33342 staining.

文献引用

Application Reference

Hori N et al. J Biol Chem 2020; 295 (26) : 8798-8807 Vestigial-like family member 3 (VGLL3), a cofactor for TEAD transcription factors, promotes cancer cell proliferation by activating the Hippo pathway.
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产品评论

Strep epitope Tag antibody [GT661] 目录号 GTX628900

Rating
( Average 5 based on 1 users reviews)
实验应用
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-Strep epitope Tag antibody [GT661] used in Western Blot (WB). GTX628900
Date : Anonymous submitted on 30-Apr-2019
Score :
Application Tested : WB
Sample : Strep-tagged protein expressing eHAP cells
Amount used : 10 μg
Blocking : 1% skim milk in PBS, 25°C, 2Hr
Primary Antibody : 1:1000, 4°C, 14Hr
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SDS
PBS.pdf
Glycerol.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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