Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Strep epitope Tag antibody [HL1252] (GTX636654) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunoprecipitation of Strep epitope Tag protein from 293T whole cell extracts using 5 μg of Strep epitope Tag antibody [HL1252] (GTX636654).
Western blot analysis was performed using Strep epitope Tag antibody [HL1252] (GTX636654).
EasyBlot HRP-conjugated anti rabbit IgG antibody (GTX221666-01).

Strep epitope Tag antibody [HL1252] detects Strep epitope Tag protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Strep epitope Tag stained by Strep epitope Tag antibody [HL1252] (GTX636654) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).