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Streptavidin

目录号 GTX30948

实验应用

ELISA, Conjugation
产品包装
1 mg (¥1300)
产品说明

摘要

Streptavidin is a 55 kDa (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC). Extinction Coefficient 1% A280=32.0. Protein content: > 95%.

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
ELISA Assay dependent
Conjugation Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

产品属性

形式

Liquid

存储溶液

0.1M Bicarbonate (pH8.0)

保存剂

0.05% Sodium azide

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

功能与背景

Streptavidin is a 55 kDa (subunit MW 14 kDa) biotin-binding protein isolated from Streptomyces avidini. Streptavidin is superior to avidin, because it does not contain carbohydrate like avidin and has no net charge at neutral pH. Streptavidin~biotin system is routinely used in Immunohistochemistry (IHC). Extinction Coefficient 1% A280=32.0. Purity: > 99% pure by HPLC; Eletrophoretically homogenous, single band. Protein content: > 95%; Activity 14.8 units/ mg, one unit binds 1.0 microgram of d-biotin; Protease activity: 0.000018 units/mg; Isoelectric point: 7.0 +/- determined by Isoelectric focusing.

研究领域

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SDS
Sodium Azide.pdf
产品包装 报价 (¥)
¥ 1300
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