产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:1000 |
| 1:50 - 1:200 |
| 1:50 - 1:200 |
| Assay dependent |
| 1 μg/ml |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
This antibody is specific for the cytoplasmic form of Survivin.
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.9 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Full length recombinant human Survivin [UniProt# O15392]
纯化方式
Protein G purified
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
baculoviral IAP repeat containing 5 , API4 , EPR-1
细胞定位
Cytoplasm,Nucleus
功能与背景
This gene is a member of the inhibitor of apoptosis (IAP) gene family, which encode negative regulatory proteins that prevent apoptotic cell death. IAP family members usually contain multiple baculovirus IAP repeat (BIR) domains, but this gene encodes proteins with only a single BIR domain. The encoded proteins also lack a C-terminus RING finger domain. Gene expression is high during fetal development and in most tumors yet low in adult tissues. Antisense transcripts are involved in the regulation of this gene's expression. At least four transcript variants encoding distinct isoforms have been found for this gene, but the full-length natures of only three of them have been determined. [provided by RefSeq]
数据库
研究领域
產品图片
![Anti-Survivin antibody [60.11] used in Western Blot (WB). GTX24470](/templates/default/js/lightbox/images/loading.gif "Anti-Survivin antibody [60.11] used in Western Blot (WB). GTX24470")
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GTX24470 WB Image
WB analysis of mock or overexpressing Survivin cell lysate using GTX24470 Survivin antibody [60.11].
Loading : 5μg
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GTX24470 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX24470 Survivin antibody [60.11].
Green : primary antibody
Blue : Hoechst 33258
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GTX24470 IHC-P Image
IHC-P analysis of human ovary cancer tissue using GTX24470 Survivin antibody [60.11].
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GTX24470 FACS Image
FACS (Intracellular staining) analysis of U2OS cells using GTX24470 Survivin antibody [60.11].
Blue : Primary antibody
Orange : Isotype control
Dilution : 5 μg/mL
Fixation : 4% PFA
Permibilization : 0.1% saponin
|
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GTX24470 FACS Image
FACS (Intracellular staining) analysis of A549 cells using GTX24470 Survivin antibody [60.11].
Blue : Primary antibody
Orange : Isotype control
Dilution : 1 μg/mL
Fixation : 4% PFA
Permibilization : 0.1% saponin
|
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GTX24470 ICC/IF Image
ICC/IF analysis of HeLa cells using GTX24470 Survivin antibody [60.11].
Green : primary antibody
Blue : Hoechst 33258
|
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GTX24470 WB Image
WB analysis of HeLa cell lysate (30μg) using GTX24470 Survivin antibody [60.11].
Loading : 30μg
Dilution : 1 μg/ml
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GTX24470 FACS Image
FACS (Intracellular staining) analysis of Daudi cells using GTX24470 Survivin antibody [60.11].
Blue : Primary antibody
Orange : isotype control
Dilution : 1 μg/mL
Fixation : 4% PFA
Permibilization : 0.1% saponin
|
文献引用
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产品评论
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![Anti-Survivin antibody [60.11] used in Western Blot (WB). GTX24470](/upload/website/prouct_img/small/GTX24470/GTX24470_1289_WB_w_23060722_403.webp "Anti-Survivin antibody [60.11] used in Western Blot (WB). GTX24470")
![Anti-Survivin antibody [60.11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24470](/upload/website/prouct_img/small/GTX24470/GTX24470_161_ICC-IF_w_23060722_174.webp "Anti-Survivin antibody [60.11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24470")
![Anti-Survivin antibody [60.11] used in IHC (Paraffin sections) (IHC-P). GTX24470](/upload/website/prouct_img/small/GTX24470/GTX24470_523_IHC-P_w_23060722_904.webp "Anti-Survivin antibody [60.11] used in IHC (Paraffin sections) (IHC-P). GTX24470")
![Anti-Survivin antibody [60.11] used in Flow cytometry (FACS). GTX24470](/upload/website/prouct_img/small/GTX24470/GTX24470_FACS_2_w_23060722_949.webp "Anti-Survivin antibody [60.11] used in Flow cytometry (FACS). GTX24470")
![Anti-Survivin antibody [60.11] used in Flow cytometry (FACS). GTX24470](/upload/website/prouct_img/small/GTX24470/GTX24470_FACS_1_w_23060722_418.webp "Anti-Survivin antibody [60.11] used in Flow cytometry (FACS). GTX24470")
![Anti-Survivin antibody [60.11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24470](/upload/website/prouct_img/small/GTX24470/GTX24470_160_ICC-IF_w_23060722_879.webp "Anti-Survivin antibody [60.11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24470")
![Anti-Survivin antibody [60.11] used in Western Blot (WB). GTX24470](/upload/website/prouct_img/small/GTX24470/GTX24470_1288_WB_w_23060722_886.webp "Anti-Survivin antibody [60.11] used in Western Blot (WB). GTX24470")
![Anti-Survivin antibody [60.11] used in Flow cytometry (FACS). GTX24470](/upload/website/prouct_img/small/GTX24470/GTX24470_89_FACS_w_23060722_893.webp "Anti-Survivin antibody [60.11] used in Flow cytometry (FACS). GTX24470")
![Anti-Survivin antibody [60.11] used in Western Blot (WB). GTX24470](/upload/website/prouct_img/normal/GTX24470/GTX24470_1289_WB_w_23060722_403.webp "Anti-Survivin antibody [60.11] used in Western Blot (WB). GTX24470")
![Anti-Survivin antibody [60.11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24470](/upload/website/prouct_img/normal/GTX24470/GTX24470_161_ICC-IF_w_23060722_174.webp "Anti-Survivin antibody [60.11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24470")
![Anti-Survivin antibody [60.11] used in IHC (Paraffin sections) (IHC-P). GTX24470](/upload/website/prouct_img/normal/GTX24470/GTX24470_523_IHC-P_w_23060722_904.webp "Anti-Survivin antibody [60.11] used in IHC (Paraffin sections) (IHC-P). GTX24470")
![Anti-Survivin antibody [60.11] used in Flow cytometry (FACS). GTX24470](/upload/website/prouct_img/normal/GTX24470/GTX24470_FACS_2_w_23060722_949.webp "Anti-Survivin antibody [60.11] used in Flow cytometry (FACS). GTX24470")
![Anti-Survivin antibody [60.11] used in Flow cytometry (FACS). GTX24470](/upload/website/prouct_img/normal/GTX24470/GTX24470_FACS_1_w_23060722_418.webp "Anti-Survivin antibody [60.11] used in Flow cytometry (FACS). GTX24470")
![Anti-Survivin antibody [60.11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24470](/upload/website/prouct_img/normal/GTX24470/GTX24470_160_ICC-IF_w_23060722_879.webp "Anti-Survivin antibody [60.11] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX24470")
![Anti-Survivin antibody [60.11] used in Western Blot (WB). GTX24470](/upload/website/prouct_img/normal/GTX24470/GTX24470_1288_WB_w_23060722_886.webp "Anti-Survivin antibody [60.11] used in Western Blot (WB). GTX24470")
![Anti-Survivin antibody [60.11] used in Flow cytometry (FACS). GTX24470](/upload/website/prouct_img/normal/GTX24470/GTX24470_89_FACS_w_23060722_893.webp "Anti-Survivin antibody [60.11] used in Flow cytometry (FACS). GTX24470")
