产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
Raji
预测种属反应
Mouse, Rat, Bovine, Pig, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
2.83 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human Syk. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
From tissue culture supernatant
偶联
Unconjugated
RRID
AB_2888388
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
spleen associated tyrosine kinase , p72-Syk
细胞定位
Cell membrane,Cytoplasm, cytosol
功能与背景
This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2010]
数据库
研究领域
產品图片
![Anti-Syk antibody [GT351] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633910](/templates/default/js/lightbox/images/loading.gif "Anti-Syk antibody [GT351] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633910")
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GTX633910 ICC/IF Image
Syk antibody [GT351] detects Syk protein at cytoplasm and cell membrane by immunofluorescent analysis.Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Syk stained by Syk antibody [GT351] (GTX633910) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).Scale bar= 10 μm.
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GTX633910 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Syk antibody [GT351] (GTX633910) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
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文献引用
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产品评论
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![Anti-Syk antibody [GT351] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633910](/upload/website/prouct_img/small/GTX633910/GTX633910_43314_20190109_ICC_IF_w_23061202_109.webp "Anti-Syk antibody [GT351] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633910")
![Anti-Syk antibody [GT351] used in Western Blot (WB). GTX633910](/upload/website/prouct_img/small/GTX633910/GTX633910_43314_20180817_WB_w_23061202_450.webp "Anti-Syk antibody [GT351] used in Western Blot (WB). GTX633910")
![Anti-Syk antibody [GT351] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633910](/upload/website/prouct_img/normal/GTX633910/GTX633910_43314_20190109_ICC_IF_w_23061202_109.webp "Anti-Syk antibody [GT351] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633910")
![Anti-Syk antibody [GT351] used in Western Blot (WB). GTX633910](/upload/website/prouct_img/normal/GTX633910/GTX633910_43314_20180817_WB_w_23061202_450.webp "Anti-Syk antibody [GT351] used in Western Blot (WB). GTX633910")
