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Syk抗体 [N2C2], Internal

Anti-Syk antibody [N2C2], Internal used in Western Blot (WB). GTX100748
Anti-Syk antibody [N2C2], Internal used in Western Blot (WB). GTX100748
Anti-Syk antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100748
Anti-Syk antibody [N2C2], Internal used in Immunoprecipitation (IP). GTX100748
Anti-Syk antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100748
Anti-Syk antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100748
Anti-Syk antibody [N2C2], Internal used in Western Blot (WB). GTX100748
Anti-Syk antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100748
Anti-Syk antibody [N2C2], Internal used in Western Blot (WB). GTX100748

目录号 GTX100748

文献引用 4

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IP

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 Syk 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

72 kDa. ( Note )

阳性对照

mouse BMDM cells , mouse spleen , Raji , A431

预测种属反应

Bovine, Pig, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.32 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human Syk. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1952121

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

spleen associated tyrosine kinase , p72-Syk

功能与背景

This gene encodes a member of the family of non-receptor type Tyr protein kinases. This protein is widely expressed in hematopoietic cells and is involved in coupling activated immunoreceptors to downstream signaling events that mediate diverse cellular responses, including proliferation, differentiation, and phagocytosis. It is thought to be a modulator of epithelial cell growth and a potential tumour suppressor in human breast carcinomas. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Syk antibody [N2C2], Internal used in Western Blot (WB). GTX100748

GTX100748 WB Image

SYK antibody [N2C2], Internal detects SYK protein by western blot analysis.
A. 30 μg mouse BMDM (bone marrow-derived macrophage) cells
B. 30 μg mouse Syk null cells
10% SDS-PAGE
SYK antibody [N2C2], Internal (GTX100748) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Syk antibody [N2C2], Internal used in Western Blot (WB). GTX100748

GTX100748 WB Image

SYK antibody detects SYK protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SYK antibody (GTX100748) at a dilution of 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Syk antibody [N2C2], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100748

GTX100748 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using SYK(GTX100748) antibody at 1:200 dilution.

Anti-Syk antibody [N2C2], Internal used in Immunoprecipitation (IP). GTX100748

GTX100748 IP Image

Immunoprecipitation of SYK protein from A431 whole cell extracts using 5 μg of SYK antibody [N2C2] (GTX100748).
Western blot analysis was performed using SYK antibody [N2C2] (GTX100748).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Syk antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100748

GTX100748 IHC-P Image

SYK antibody [N2C2], Internal detects SYK protein at cytoplasm in human endometrium by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrium.
SYK antibody [N2C2], Internal (GTX100748) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Syk antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100748

GTX100748 IHC-P Image

SYK antibody [N2C2], Internal detects SYK protein at cytosol on rat spleen by immunohistochemical analysis.
Sample: Paraffin-embedded rat spleen.
SYK antibody [N2C2], Internal (GTX100748) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Syk antibody [N2C2], Internal used in Western Blot (WB). GTX100748

GTX100748 WB Image

SYK antibody detects SYK protein by western blot analysis. Mouse tissue extracts (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with SYK antibody (GTX100748) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Syk antibody [N2C2], Internal used in IHC (Paraffin sections) (IHC-P). GTX100748

GTX100748 IHC-P Image

SYK antibody [N2C2], Internal detects SYK protein at cytoplasm in human cervical cancer by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical cancer.
SYK antibody [N2C2], Internal (GTX100748) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Syk antibody [N2C2], Internal used in Western Blot (WB). GTX100748

GTX100748 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with Syk antibody [N2C2], Internal (GTX100748) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

文献引用

Application Reference

Tsukui D et al. iScience 2023; 26 (8) : 107293 GM-CSF receptor/SYK/JNK/FOXO1/CD11c signaling promotes atherosclerosis.
Jia Y et al. Cell Death Dis 2019; 10 (2) : 38 Tetrandrine enhances the ubiquitination and degradation of Syk through an AhR-c-src-c-Cbl pathway and consequently inhibits osteoclastogenesis and bone destruction in arthritis.
实验应用 : WB
种属反应 : Mouse
Jia Y et al. FASEB J 2018; : fj201701148RR Tetrandrine attenuates the bone erosion in collagen-induced arthritis rats by inhibiting osteoclastogenesis via spleen tyrosine kinase.
实验应用 : WB
种属反应 : Rat
Tomoyuki Mukai et al. J Bone Miner Res 2014; 29 (12) : 2618-2635 SH3BP2 Cherubism Mutation Potentiates TNF‐α–Induced Osteoclastogenesis via NFATc1 and TNF‐α–Mediated Inflammatory Bone Loss.
实验应用 : WB
种属反应 : Mouse
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